Labshake search
Citations for GE Life Sciences :
851 - 900 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... followed by Step-tag affinity chromatography using StepTactin resin (GE Healthcare) in BRB20 ...
-
bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
-
bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by staining with Coomassie Brilliant Blue R250 or by transferring to a PVDF membrane (GE Life Sciences), and staining with Ponceau S dye or detection by immunoblotting ...
-
bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
-
bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
-
bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences).
-
bioRxiv - Cell Biology 2021Quote: ... followed by transfer of proteins from gel to polyvinylidene fluoride (PVDF) membrane (Amersham Hybond P 0.45, GE Healthcare Life Sciences) and sequential use of primary and secondary antibodies that are listed in Table 3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against phospho-Ser935 LRRK2 rabbit monoclonal (1/1000 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
-
bioRxiv - Microbiology 2021Quote: ... After gentle stirring for one hour aggregates were removed by centrifugation at 75,600g and the solubilised membrane proteins were applied to a StrepTrap column (GE healthcare) which was then washed in TBS supplemented with 0.01% LMNG ...
-
bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
-
bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
-
bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein extracts underwent vacuum filtration through a pre-wet 0.2 μm pore size nitrocellulose membrane (GE Healthcare) for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: Proteins (10 μg) were separated by 10%–12.5% SDS-PAGE and electroblotted onto a 45-μm nitrocellulose membrane (GE Healthcare). Membranes were stained with Ponceau to ensure equal distribution of proteins in each lane ...
-
bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
-
bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
-
bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
-
bioRxiv - Molecular Biology 2022Quote: ... the crude lysate proteins were separated by Tricine-SDS PAGE (16%) and blotted onto a PVDF membrane (AmerhamTMHybondTM, 0.2 µM PVDF; GE Healthcare Life Science ...
-
bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were resolved on 6% SDS-PAGE gel and transferred to a nitrocellulose membrane (0.45 μm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
-
bioRxiv - Plant Biology 2022Quote: ... Fifty micrograms of proteins were separated by SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE Healthcare). The membrane was probed with rabbit polyclonal antibodies against maize calreticulin ...
-
bioRxiv - Genomics 2022Quote: ... Separation was done on 8% bis-acrylamide gels in Tris-Glycine-SDS buffer and proteins were transferred on Amersham Protran 0.2 µm NC membranes (GE Healthcare) for western blotting ...
-
bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
-
bioRxiv - Immunology 2023Quote: ... The separated proteins were blotted onto a nitrocellulose membrane (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Freiburg, Germany), blocked for the unspecific binding with 5% non-fatty milk and incubated overnight at 4 °C with the respective primary antibody ...
-
bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins (30 µg/lane) were resolved by SDS-PAGE and electro-transferred to the Hybond-P PVDF membranes (GE Healthcare). Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins from extracts were separated on SDS polyacrylamide gels and blotted onto Nitrocellulose membrane (Hybond ECL Cat#RPN2020D, GE Healthcare). Membranes were blocked using Skimmed Milk 5% ...
-
bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
-
bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
-
bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
-
bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was fractionated using a Hi-Trap Ni2+ column (GE Healthcare) following the manufacturer’s instructions and checked by SDS-PAGE electrophoresis ...
-
bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
-
bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
-
bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then run through an equilibrated His-trap (GE healthcare) in an AKTA start fast protein liquid chromatography system ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a 5mL His-trap column (GE Healthcare) for protein purification and eluted with a linear imidazole (20 mM-200 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was purified by His TrapTM FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
-
bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
-
bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...