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Citations for GE Life Sciences :
1101 - 1150 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... transferred to membranes (Protran nitrocellulose hybridisation transfer membrane; pore size, 0.2 μm; 10600001; GE Healthcare), and blocked for 1 h at room temperature in 5% milk or 5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Biophysics 2019Quote: ... The 12xHis-tag was removed by overnight digestion with 3C protease (GE Healthcare Life Sciences) followed by a purification step with Ni-NTA agarose (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... that had encoded an N-terminal His6-tag followed by a PreScission protease (GE Healthcare) cleavage site ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using strong anion exchange (HiTrapQ and MonoQ, GE Life Sciences), followed by hydroxyapatite (CHT-II ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21(DE3) pLysS cells and purified using glutathione agarose beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Cell Biology 2021Quote: ... 2015) and transferred to a nitrocellulose membrane (Amersham Protran; 0.45-μm pore nitrocellulose membrane; GE Healthcare) using a Trans-Blot device (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... Sandwich the membrane-gel-membrane assembly between two sheets of Whatman paper (GE Healthcare #3030-917). Dry this assembly between paper towels for 1hr ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion V5-Ydj1 was subjected to site-specific cleavage with PreScission Protease (GE Healthcare) to remove the GST tag ...
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bioRxiv - Biophysics 2020Quote: Hsc70 and MTB-Hsp70 were purified first by Ni-NTA column chromatography (His-trap, GE Healthcare). The column was washed with 50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... RSFPs were isolated from the supernatant with the His SpinTrap kit (GE Healthcare, Chicago, IL, USA) and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius ...
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bioRxiv - Biochemistry 2020Quote: ... The excessive imidazole was removed using a desalting column (Hi-Prep™ 26/10, GE Healthcare). The 8 × His-SUMO was cleaved by TEV proteinase and the cleaved fragment was removed through the affinity of the His-tag to a Ni-NTA column ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a heparin column (Hi-trap Heparin HP, GE Healthcare Life Sciences) and MatP was eluted with a NaCl gradient (0.3 to 1M ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified cell-free extracts were loaded onto a His-Trap FF crude column (GE Healthcare) preequilibrated with buffer B containing 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... His-SEPT2-mCherry-SEPT6/7-strep was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... an initial IMAC step was performed on a His-Trap column (all columns by GE Healthcare) using buffers A (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Cell Biology 2022Quote: ... the elution fractions containing His-ZNF524 were further purified on a HiTrap Heparin HPTM (GE Healthcare). Therefore ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Plant Biology 2020Quote: ... electroblotted to PVDF membrane (GE Healthcare), blocked in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Synthetic Biology 2019Quote: ... transferred to nitrocellulose membrane (GE Healthcare), and immunoblotting was performed using anti-GFP-HRP (sc-9996 HRP ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred to PVDF membranes (GE Healthcare). The PVDF membranes were blocked with 5% nonfat dry milk for 1 hour at RT and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Hybond positively charged membrane (GE Healthcare), and gel dryer filter paper (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
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bioRxiv - Neuroscience 2020Quote: ... transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% milk ...
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bioRxiv - Biochemistry 2020Quote: ... HyBond positively charged membrane (GE Healthcare) and 0.45 μm Tuffryn membrane filter paper (PALL Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... transferred onto nitrocellulose membranes (GE Healthcare) and blocked in 5% non-fat milk in PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2020Quote: ... electrotransferred to nitrocellulose membrane (GE Healthcare), and immunoblotted as indicated ...
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bioRxiv - Biochemistry 2020Quote: ... HyBond positively charged membrane (GE Healthcare), thick filter paper (Bio-Rad gel dryer filter paper) ...
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bioRxiv - Microbiology 2022Quote: ... transferred onto nitrocellulose membranes (GE Healthcare) and blocked in 5% non-fat milk in PBS with 0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Nitrocellulose membranes (GE Healthcare Life Sciences) were probed with anti-GFP (clones 7.1 and 13.1 ...