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Citations for GE Life Sciences :
6601 - 6650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Pathology 2023Quote: ... immunoreactive bands were visualized using the enhanced chemiluminescence (ECL) detection reagent (GE Healthcare, Chicago, IL, USA, RPN2106) or acquired in the ImageQuant LAS 4000 device using the Immobilon Forte Western HRP substrate (WBLUF0100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Radioactivity was quantified using ImageQuant™ TL analysis software (GE Healthcare Europe GmbH, Freiburg, Germany). Enzyme activity was calculated as a percentage of radioactivity incorporated into the product with respect to the total radioactivity.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... TLC spots were exposed to a phosphor screen and detected by autoradiography using Typhoon™ FLA-7000 PhosphorImager (GE Healthcare, Uppsala, Sweden). Radioactivity was quantified using ImageQuant™ TL analysis software (GE Healthcare Europe GmbH ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: Affinity purified samples were further purified by size exclusion chromatography over a Superdex 200 10/300 GL column (GE Healthcare) with running buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The immunoblots were developed using the ECL PrimeWestern blotting detection system kit (GE Healthcare, Milan, Italy). Band intensities were quantified and expressed as optical density on a Gel Doc 2000 Apparatus (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Bioengineering 2023Quote: ... cored vertebral specimens were scanned using quantitative micro-CT (MS-8, GE Healthcare, London, Ontario, Canada) with 70 kVp X-ray tube peak potential ...
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bioRxiv - Biophysics 2023Quote: ... operated via an ÄKTA™ FPLC system (GE Healthcare). The column was equilibrated with a binding buffer consisting of 20 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% FBS (GE Healthcare -HyClone), 2mM L- glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and stored in liquid nitrogen until further use.
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Cancer Biology 2023Quote: Cellular RNA was extracted using RNAspin Mini RNA Isolation Kit (GE Healthcare; #25-0500-71) and cDNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
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bioRxiv - Biochemistry 2023Quote: ... Eluates containing myrArf1•GDP were further purified by size exclusion chromatography (SEC) using a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) pre-equilibrated and then run in an isocratic manner using fresh TNMD buffer at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Immunology 2023Quote: ... a Percoll (GE Healthcare) gradient step was included before plating ...
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bioRxiv - Biophysics 2023Quote: ... Fluorescence images of oocyte chromosomes in utero were taken in the DAPI channel on a DeltaVision RT imaging system (Applied Precision, LLC; IX70 Olympus) equipped with a charge-coupled device camera (CoolSNAP HQ ...
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bioRxiv - Cancer Biology 2023Quote: Samples were separated by SDS-PAGE prior to being transferred onto a nitrocellulose membrane (0.45µ, GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of supernatant from each sample were mixed with 30 μl of pre-washed streptavidin-sepharose beads (GE Healthcare, 17-5113-01) and incubated for 3 hours at 4°C on a nutator ...
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bioRxiv - Cell Biology 2023Quote: ... Data were visualized using a phosphorimager (Molecular Dynamics) and quantitated using ImageQuant (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the protein was treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a series of chromatography purification using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... followed by a MonoQ anion exchange column (GE Life Sciences). Peak fractions were dialyzed into Fhod storage buffer (10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... the cleared lysate was incubated with 500 µL glutathione agarose slurry (GE Healthcare, #17-0756-01) (resuspended 1:1 in lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was loaded onto a Superdex200 10/300 GL Increase column (GE Healthcare), equilibrated with 200 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare, RPN2232), and visualized in a Vilber-Lourmat Fusion Solo S chamber.
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bioRxiv - Cell Biology 2023Quote: GST and GST-DysSR22-23 were produced in BL21 bacteria using a standard protocol and were purified on Glutathione Sepharose 4B (GE Healthcare) [55] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by filtration (through 100 μm mesh) and enrichment for leukocytes by gradient centrifugation (40% Percoll GE healthcare, 600 x g, 15 min). To prepare single cell suspensions from spleen tissues ...
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins and SP3 beads (GE healthcare) were mixed in a 1:10 weight ratio in 80 % ethanol for 5 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was further purified on a MonoS Sepharose column (GE healthcare) applying a gradient of 50 to 500 mM KCl in 25 mM Hepes-KOH (pH 7.4).
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bioRxiv - Neuroscience 2023Quote: ... The liposome suspension was desalted using a PD10 column (GE Healthcare) equilibrated with desalting buffer 1 (1 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2023Quote: ... by over-night cleavage with Prescission protease (GE healthcare) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was further purified on a MonoQ Sepharose column (GE healthcare) applying a gradient of 50 to 500 mM KCl in 25 mM HEPES-KOH (pH 7.4).
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bioRxiv - Cell Biology 2023Quote: ... protein solutions were further concentrated to ∼ 100 µM and purified by size exclusion with a Superdex 200 column (SD200 16/60) on an AEKTA purifier (GE Life Sciences) in the final storage buffer (200 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were added to a HiTrap Chelating HP 5 ml column (GE Healthcare) equilibrated with buffer A (20 mM Hepes pH 7 ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Cell Biology 2023Quote: ... TbSmee1(1-400) concentrates were applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated in dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...
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bioRxiv - Biochemistry 2023Quote: ... Hitrip SP (GE Healthcare) and Superdex 200 Increase 10/300 (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The culture supernatant was incubated with Ni-Sepharose 6 Fast Flow resin (GE Healthcare) for 6-8 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Images were deconvolved using the SoftWoRx Suite (Applied Precision, GE) or the proprietary Microvolution software in Slidebook (Intelligent Imaging Innovations ...
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bioRxiv - Cell Biology 2023Quote: ... Dried membranes were exposed to BAS storage phosphor screens (Cytiva, 28956475) and scanned using a phosphor imager (Typhoon FLA 9500 GE Healthcare). Signals were analyzed with the ImageQuant TL software (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Cancer Biology 2023Quote: ... Imaging of membranes for chemiluminescence or after ponceau S staining was performed using the Amersham Imager 600 (GE HealthCare Life Sciences, USA). Quantification of protein expression was performed using ImageJ software ...
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bioRxiv - Biochemistry 2023Quote: ... SEC was performed on a HiLoad 16/60 Superdex 200 pg SEC column (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...