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Citations for GE Life Sciences :
5701 - 5750 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare). PBMC were frozen in 10% DMSO/FBS and stored in liquid nitrogen until analysis ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were separated from buffy coats by density gradient centrifugation (Ficoll Paque, GE Healthcare). NK cells were then isolated from PBMCs by negative selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were subjected to a density gradient centrifugation using a 37% Percoll solution (GE Healthcare Life Sciences). Erythrocytes were lysed with NH4Cl.
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired every 10 min for 12 h using an IN Cell Analyzer 2000 microscope (GE Healthcare; Nikon 20x/0.45 NA Plan Fluor objective ...
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bioRxiv - Immunology 2022Quote: ... Austria) and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque, GE Healthcare, ThermoFisher). The PBMCs were washed and cryopreserved in freezing medium (8% DMSO in FBS ...
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bioRxiv - Immunology 2022Quote: ... LP immune cells were enriched at the interface of a 40%/80% Percoll gradient (GE Healthcare Life Sciences), washed and stained for flow cytometry with the same antibodies used in whole blood ...
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bioRxiv - Microbiology 2024Quote: ... and the cells were used for RNA extraction using the Illustra RNAspin Mini RNA isolation kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96-well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20x objective (GE Healthcare). Images were batch processed through an imaging processing software ...
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bioRxiv - Neuroscience 2023Quote: ... The collected cell suspension was mixed with isotonic Percoll (GE Healthcare, 90% Percoll 10% 10x PBS, pH 7.2) at a final concentration of 22.5% and centrifuged at 800 g for 20 min without break at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20X objective (GE Healthcare). Neuronal mitoSOX signal was detected based on the overlap of mitoSOX with TUBB3 ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats with Ficoll-Paque (GE Healthcare, Pittsburgh, PA). Subsequently ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96-well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Immune cells were isolated using a Ficoll gradient as described by the manufacturer (GE Healthcare, GE17-0891-01). The resulting immune cells were resuspended in 1x PBS prior to being plated in a 96-well plate for flow staining.
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells were isolated from buffy coats by density centrifugation using Ficoll-Paque Plus (GE Healthcare). CD14+ monocytes were subsequently isolated using magnetic-activated cell sorting (Miltenyi Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples using Ficoll-Paque (GE Healthcare, Cat# 17144003) and gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Immunology 2023Quote: ... Hepatic parenchymal cells were removed by density gradient centrifugation at 700 × g in 33.75% Percoll PLUS (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... cells were imaged using a 60x oil immersion objective lens on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20X objective (GE Healthcare). Neuronal γH2AX signal was detected based on the overlap of γH2AX with TUBB3 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed three times with PBS and then imaged in PBS with the IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification ...
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bioRxiv - Microbiology 2024Quote: ... The infection was assessed by using an IN CELL Analyzer 6500 high-throughput automated confocal microscope (Ge Healthcare) located in BSL-3 safety laboratory ...
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bioRxiv - Cell Biology 2024Quote: Live-cell fluorescence imaging was performed using a Delta Vision Elite widefield fluorescence microscope (GE Healthcare Life Science) equipped with a 100× PlanAPO oil-immersion objective lens (Olympus ...
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bioRxiv - Cancer Biology 2024Quote: ... Mononuclear cells were extracted from bone marrow samples using Ficoll-Paque™ PLUS (GE Healthcare #17-1440-02), followed by red blood cell lysis using a red blood lysis buffer (Sigma #R7757).
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bioRxiv - Biochemistry 2024Quote: ... Clear cell lysates (clarified by centrifugation) were purified with Ni-HiTrap FF and MBPtrap HP columns (GE Healthcare). Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were imaged using a UPLSAPO 100x / 1.4NA oil objective on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Plant Biology 2020Quote: ... The PuACO1 or PuERF2 CDSs were inserted into the pGEX4T-1 vector (GE Healthcare, http://www3.gehealthcare.com) downstream from GST ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNA encoding mouse TRIM41 (residue 35-85 aa, NP_663352.2) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-conjugated sheep anti-mouse IgG antibody diluted in 1:4000 (NA931, GE Healthcare, Little Chalfont, UK); and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934 ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with a 1:5000 dilution of anti-rabbit (GE Healthcare, Little Chalfont, UK, NA934V) or anti-mouse secondary antibody (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol) and further purified by gel filtration over a Superdex-200 column (GE Healthcare). The protein product was concentrated with a centrifugal filter unit (MD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... the sample supernatant fraction was incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, 17-0756-01) for 3 h at 4 °C to bind GST-tagged proteins ...
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bioRxiv - Bioengineering 2019Quote: ... Whatman Grade 1 chromatography paper for the paper-based reactions was obtained from GE Healthcare (Mumbai, India). DNA ladder (O’GeneRuler ultra low range 10–300 bp ladder) ...
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bioRxiv - Molecular Biology 2020Quote: ... 12.5% acrylamide:bis-acrylamide 19:1) electrophoresis and heat/vacuum dried for autoradiography (Amersham Hyperfilm ECL, GE Healthcare). Images were analyzed using ImageJ ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM β-mercaptoethanol and loaded onto a 1 ml MonoQ anion exchange chromatography column (GE Healthcare). After washing with 10 column volumes of buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HRP conjugated secondary antibodies were used at 1:10,000 and visualised using ECL (GE Healthcare, Buckinghamshire, UK), high performance chemiluminescence film (Amersham Hyperfilm ECL ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Ban quantification was done using Image LabTM v ...
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bioRxiv - Biochemistry 2021Quote: Calorimetric titration of Mint3NT with FIH-1 was performed using a MicroCal™ iTC200 system (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) was subcloned into the BamHI and XhoI sites of pGEX-4T-1 vector (GE Healthcare, Inc.) using standard PCR-based protocols ...
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bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...