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Citations for GE Life Sciences :
5551 - 5600 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: NDC80-yeGFP expressing cells were imaged at room temperature using a DeltaVision Elite (eDV) microscope (Applied Precision) equipped with a PCO Edge5.5 sCMOS camera and Olympus 60X oil-immersion objective lens (Plan Apo N ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysate was applied to a nickel affinity column (GE Healthcare; Sepharose high performance beads) equilibrated in Buffer A and purified by FPLC ...
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bioRxiv - Neuroscience 2023Quote: Live imaging of GFP immunofluorescence in hCOs was obtained using an IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification.
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted using the Illustra RNAspin kit with DNaseI treatment (GE Healthcare). RT-PCR was performed with 100 ng of total RNA using SuperScript III One-Step RT-PCR System (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were precipitated by Ficoll sedimentation according to manufacturer’s instructions (Ficoll Paque Plus, GE Healthcare). Remaining cells were washed with HBSS (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Cells were mounted on a slide and imaged using DeltaVision Elite wide-field fluorescence microscope (GE Healthcare) with a 60x/1.516 oil immersion objective ...
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bioRxiv - Cell Biology 2023Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Directly before imaging ...
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bioRxiv - Immunology 2024Quote: ... The cells were then subjected to two-phase density separation with upper 35% Percoll PLUS (GE Healthcare) (1000g ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies for chemiluminescence detection were donkey anti-rabbit-HRP (GE healthcare, NA 9340V, 1:5000) or sheep anti-mouse-HRP (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... 2) the reaction mix was purified using anion exchange column (HiTrap QFF 1 mL; GE Healthcare); 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT buffer and was further purified on Heparin column HP Trap 5mL (GE Healthcare) using a 200-600 mM KCl gradient ...
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bioRxiv - Immunology 2019Quote: ... reaction mixture was applied to a 1 mL HiTrap KappaSelect affinity column (GE Healthcare Life Sciences). All affinity purification steps were carried out by connecting the affinity column to a peristaltic pump with flow rate of 1ml/min ...
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bioRxiv - Molecular Biology 2020Quote: ... Ion exchange experiments were performed on a Hitrap™ Q HP (1 mL) column (GE healthcare) with ion exchange buffer A and ion exchange buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... The αVKOR blot was then incubated with 1:10,000 secondary anti-mouse-HRP (GE Healthcare NA931V) + 1X TBS-T 0.1% Tween + 5% Milk for one hour ...
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bioRxiv - Biochemistry 2019Quote: ... and hRIO2 (1-353) Δ(131-146) were further purified with a HiQ-Sepharose (GE Healthcare) chromatography ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA were extracted using the Illustra RNAspin kit and treated with DNase 1 (GE Healthcare). cDNA synthesis and taqman qPCR were performed as previously described [16] ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were detected using mouse IgG HRP linked whole Ab (1:4000; NXA931; GE healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Reaction products were separated by ion-exchange chromatography (1 mL Hitrap Q HP, GE Life Sciences) using a gradient from 0.2 to 2 M ammonium acetate ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour at room temperature before being revealed using ECL Prime detection reagent (GE Healthcare) and chemiluminescence reading on a luminescent image analyzer (LAS-4000 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used 1% agarose gel electrophoresis and a NanoVue spectrophotometer (GE Healthcare Life Sciences, Buckinghamshire, UK). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared supernatant was loaded onto two HisTrap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... 25 mM HEPES/NaOH pH 8) into a 1 mL HiTrap Heparin HP column (GE Healthcare). This column was further washed with buffer NT.A ...
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bioRxiv - Neuroscience 2020Quote: ... sections for IHC were treated with streptavidin-horseradish peroxidase conjugate (GE Healthcare, Amersham, UK; 1:200) prior to visualization using metal enhanced chromagen ...
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bioRxiv - Biophysics 2021Quote: ... 1□mM DTT and 10% glycerol) using a PD MiniTrap G-25 sephadex columns (GE Healthcare) and the purified proteins were flash frozen in liquid N2 and stored at −80□°C ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution and an S200 16/60 gel filtration column (Pharmacia Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom). The enzyme was dialysed into 20 mM Bis-Tris propane ...
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bioRxiv - Neuroscience 2021Quote: ... Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000, NA931 GE Healthcare) diluted in 5% milk ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... followed by horse radish peroxidase (HRP)-linked donkey anti-rabbit IgG (1:2,000; GE Healthcare, NA9340V) for 2h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was supplemented with 1 mM DTT and applied to Glutathione Sepharose 4B (GE Healthcare), and washed with 50 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT using a S-200 10/300 analytical size exclusion chromatography column (GE Healthcare) connected in-line to mini-DAWN TREOS light scattering and Optilab T-rEX refractive index detectors (Wyatt Technology) ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated with horseradish peroxidase–conjugated anti-rabbit IgG (1:10,000; NA934; GE healthcare) diluted with TBS-T for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Plant Biology 2020Quote: ... separated in 1% [w/v] agarose gels and blotted onto Amersham Hybond-XL membranes (GE Healthcare) according to the supplier’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The VHHs were purified from the solution using a 1 mL HisTrap HP column (GE Healthcare). To elute the bound VHHs a linear imidazole gradient starting from 20 mM and ending at 500 mM imidazole in binding buffer over a total volume of 20 mL was used ...
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bioRxiv - Microbiology 2019Quote: ... Eggs were collected on wet filter paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK) and desiccated for 5–10 days before hatching in de-ionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Biochemistry 2022Quote: ... either a 1:10,000 dilution of α-mouse-HRP conjugated antibody (GE Healthcare Life Sciences NA931) or a 1:2,000 dilution of α-mouse-HRP conjugated antibody (used for western blot of immunoprecipitations ...
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bioRxiv - Neuroscience 2022Quote: ... and reincubated with the secondary HRP-conjugated antibody (1:2000 dilution for anti-rabbit, GE Healthcare, NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated for 1 h with ECL mouse IgG HRP-conjugated whole antibody (GE Healthcare) or rabbit IgG HRP-conjugated whole antibody (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Soluble extracts were mixed with 1 mL of glutathione agarose beads (catalogue number # 17075601, GE Healthcare) and incubated for 2 h at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was loaded onto a Buffer-A-equilibrated 1 mL HiTrap QFF column (GE Healthcare). Then the column was washed sequentially by 10 column volumes of Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysates were loaded onto Ni-Sepharose beads and nutated for ∼1 h (GE Healthcare). Beads were washed twice with denaturing buffer and hexa-histidine-tagged proteins were eluted with the same buffer supplemented with 500 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were washed with PBST and incubated with anti-M13-HRP 1:3,000 (GE Healthcare) in blocking buffer for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... the respective supernatants were applied to 1/5 ml HiTrap™ Q XL columns (GE Healthcare) for anion exchange chromatography using the ÄKTA Start chromatography system (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... sheep anti-mouse horseradish peroxidase-linked antibody (1:3000, NA931V, GE Healthcare Life Sciences, Uppsala, Sweden), donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000 ...