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Citations for GE Life Sciences :
5901 - 5950 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: Germ cells and embryos were mounted on 2% agarose pads and imaged live using a DeltaVision Elite microscope (Applied Precision) with a 60x Silicon oil objective lens ...
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bioRxiv - Biochemistry 2019Quote: ... The PLPs were pelleted from the lysed cells and purified over a sizing column containing Sephacryl S1000 matrix (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
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bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was filtered with a 0.45-μm filter and loaded onto a nickel-charged HisTrap HP column (GE Healthcare). Proteins were eluted with elution buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
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bioRxiv - Molecular Biology 2021Quote: ... a single cell suspension of fetal liver cells was strained through 70µm mesh and layered onto density gradient separation media (Ficol Paque PLUS, GE Healthcare). After 20 minutes of centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were purified from heparinized whole blood via density centrifugation with Ficoll-Paque Premium (GE Healthcare) per manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Immune cells were enriched through incubation with ACK buffer (spleen) or through density gradient centrifugation using Ficoll-Paque (GE Healthcare) following the manufacturer’s protocol (blood and liver) ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood through Ficoll density centrifugation (Ficoll-Plaque PLUS, GE Healthcare, USA), aliquoted and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
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bioRxiv - Cell Biology 2021Quote: Procyclic (PCF) cell line Tb427 29.13 Trypanosoma brucei brucei (86) was grown in SDM-79 medium (GE Healthcare, G3344-3005) pH7.4 supplemented with 2 mg/mL hemin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
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bioRxiv - Microbiology 2022Quote: Cell densities were determined based on the optical density at 600 nm (OD600) using an Ultraspec 2100 spectrophotometer (GE Healthcare) or a Tecan Infinite M1000 microplate reader ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Germany) and exchanged to the desired cell culture medium using Sephadex G-25 PD10 desalting columns (GE Healthcare Bio-Science). The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
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bioRxiv - Microbiology 2023Quote: ... the cell supernatant was collected and soluble IgG was purified using affinity chromatography (GammaBind Plus Sepharose, GE HealthCare, Chicago, IL). Purified IgG was buffer-exchanged in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Live imaging of GFP or RFP immunofluorescence in COs and NICOs was obtained using IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation on lymphocyte separation medium (LSM 1077, GE Healthcare) as previously described191 ...
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation on Ficoll-Paque Plus (GE Healthcare, Boston, MA, USA). CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Images of mouse spermatocyte spreads testicular cell squashes were acquired using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision) with a 60ξ NA 1.4 oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were isolated from the cell suspension using a 37 % and 70 % percoll gradient (GE Healthcare Bio-Sciences AB, Sweden) diluted with HBSS (with or without phenol red ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Molecular Biology 2023Quote: The supernatant or cell extract was then filtered through a 0.45 µm pore filter and loaded on a HisTrap HP column (GE Healthcare) with immobilized Ni2+ beads equilibrated with HisBufferA (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by separation on a Ficoll-Hypaque gradient (GE Healthcare). For detailed splenocyte isolation protocol ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Biophysics 2023Quote: YidC and YidCΔC were purified from pTrc99a-YidC (Welte et al., 2012) and pTrc99a-YidCΔC expressing BL21 cells using a Ni-NTA FF crude column (GE Healthcare) on an ÄKTA chromatography system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then imaged at 4X using the InCell 2200 or 6500 automated fluorescence microscope system (GE Healthcare Life Sciences) and quantitative analysis was performed with Cell Profiler Software(182,183) ...
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bioRxiv - Immunology 2023Quote: ... from which the debris and the dead cells were removed by density gradient centrifugation with a 46% Percoll (GE Healthcare) gradient ...
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... His-TWF-1 was further purified with Superdex-75 increase 10/300 GL gel filtration column (GE Healthcare) equilibrated 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... it was passed through Whatman™ Grade 1 filter paper (diameter 150 mm) (GE Healthcare Life Sciences, Ireland), followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed with HRP-conjugated anti-mouse or anti-rabbit (1:5,000, GE Healthcare Bio-Sciences) secondary antibody after washing several times with 1X PBS for 45 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to His-JMJD6 (1-343) was loaded to 7mL of Ni-NTA resin (GE Healthcare), washed with nickel-binding buffer containing 20mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated DJ-1/Hsp32 was loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare), pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Genetics 2019Quote: ... Primary antibodies were detected by HRP-conjugated anti-rabbit IgG (GE Healthcare NA934V, Lot 9636020; 1:10000 dilution) or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 1 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 20 mM and 40 mM imidazole ...