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Citations for GE Life Sciences :
5851 - 5900 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted and subjected to DNaseI treatment using the Illustra RNAspin kit (GE Healthcare). cDNA synthesis from the RNA samples was performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems #4374966 ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lysate was clarified by centrifugation and purified by FPLC using Ni-NTA (HisTrap FF column, GE Healthcare) followed by size exclusion chromatography (HiLoad 26/60 Superdex 75 ...
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bioRxiv - Neuroscience 2024Quote: ... and numbers and intensity of pSer129 immunofluorescence staining were counted and quantified by IN Cell Developer Toolbox (GE Healthcare). The nuclear count was calculated using the Hoechst count ...
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bioRxiv - Biophysics 2020Quote: ... Cell supernatant were collected after centrifuge at 40,000 rpm for 30 min and loaded onto superpose 6 increase (GE healthcare) for FSEC analysis.
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... High-throughput imaging and quantification of fluorescence signals were carried out using the In Cell Analyzer 2000 (GE Healthcare, USA). ACE2 signal intensity was normalized to total cell number within each well as measured by DAPI staining ...
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMC) were prepared from citrated blood samples using Ficoll® Paque Plus density centrifugation (GE Healthcare). Synovial tissue was digested for 2h at 37 0C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... L cells (ATCC® CRL-2648) were cultured in high glucose Dulbecco’s modified Eagle medium (GE Healthcare, Piscataway, NJ, USA) plus 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Metaphase cells were imaged for 5 min every 7.5 s using an Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma) ...
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bioRxiv - Microbiology 2019Quote: ... Samples were separated into plasma and peripheral blood mononuclear cells (PBMCs) by centrifugation through a Ficoll-Hypaque gradient (GE Healthcare). PBMCs were cryopreserved in freezing media and stored in liquid nitrogen until further analysis by antibody repertoire sequencing.
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bioRxiv - Cell Biology 2019Quote: ... Three-dimensional image stacks of interphase cells were acquired in 0.2 μm steps using 60x NA 1.42 objective on an Olympus DeltaVision microscope (GE Healthcare, USA) equipped with DAPI/FITC/TRITC/CY5 filter set (Chroma ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Immunology 2019Quote: ... Sequentially cells were passed through a 100μM cell strainer and purified by centrifugation 30min at 2400rpm in 40/80 Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2019Quote: ... cells were passed through a 100μM cell strainer purified by centrifugation 30 minutes at 2400rpm in 40/80 Percoll (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2020Quote: ... fractions of samples produced using a stable baculovirus/SF9 cell line and purified using an AVB sepharose column (GE Healthcare) were concentrated and loaded onto a 5-40% step sucrose gradient to separate empty and full (DNA containing ...
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bioRxiv - Bioengineering 2021Quote: ... TILs were then isolated from the single cell suspension using a density gradient with Percoll Centrifugation Media (GE Life Sciences) and DMEM Media (10% FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Image stack files were analyzed on a high-content image analysis software (IN Cell Developer Toolbox v1.9, GE Life Sciences). Target set segmentation and quantification measures were developed for individual channels and applied onto all sample images ...
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bioRxiv - Molecular Biology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient sedimentation of buffy coats from healthy donors (GE Healthcare). Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... We then made use of morphological characteristics of Hoechst-labelled nuclei to distinguish dead cells [58] using INCell Analyzer 3.7 Workstation software (GE Healthcare). Cells were classified as alive or dead and % of dead cells computed for each drug-treated and control DMSO wells.
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bioRxiv - Cell Biology 2021Quote: ... The ERMS cells were maintained in RPMI1640 (Nacalai, Osaka, Japan) with 10% fetal bovine serum (HyClone; GE Healthcare, UT, USA), 100 units/ml penicillin ...
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bioRxiv - Cancer Biology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll®-Paque PLUS, GE Healthcare, 17-1440-03) from healthy donors ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Cells in the elongation zone of the root epidermis were imaged using an OMX BLAZE v4 3D SIM (Applied Precision), as described78 ...
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bioRxiv - Genetics 2020Quote: Floxed Rasa3 exon three was excised in adult (8-12 weeks of age) hematopoietic cells by inducing Mx1-Cre recombinase activity with Poly(I:C) (GE Healthcare). Mice carrying floxed Rasa3 allele(s ...
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bioRxiv - Neuroscience 2019Quote: ... with 2% FBS and 0.5 mg/mL DNase I) and endothelial cells were obtained using density gradient centrifugation with 22% Percoll (GE Healthcare) in PBS (1X) ...
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bioRxiv - Microbiology 2020Quote: Recombinant C-terminal GST-tagged hA3C and hA3C.WE-RK were expressed in HEK293T cells and purified by affinity chromatography using Glutathione Sepharose 4B beads (GE Healthcare) as described previously [65] ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed again in PBS as described and imaged using an INCELL 6500 high-content imager (GE Healthcare). Images were analyzed using the GE InCarta software.
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bioRxiv - Biochemistry 2022Quote: ... Approximately 24 hours later cells were fixed and imaged with an INCell Analyzer 2000 or 2200 (GE Healthcare Life Sciences) as described above.
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were passed through a 100 µm strainer and decidual leukocytes were enriched using a Percoll (GE Healthcare) density gradient ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Cell Biology 2020Quote: All yeast live-cell epifluorescent microscopy was performed on a Nikon Eclipse E600 or a Delta Vision microscope (Applied Precision) equipped with a Coolsnap HQ camera (Photometrics) ...
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bioRxiv - Cell Biology 2021Quote: All fixed cell images were acquired on an Inverted Olympus microscope incorporated into a GE Ultra imaging system (GE Healthcare) with SoftWoRx software (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were separated from whole blood by density-gradient centrifugation with Ficoll-Paque Plus (GE Healthcare). Neutrophils were isolated using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatant was then removed and cells were enriched using one of two Percoll-based approaches (GE Healthcare, Uppsalla, Sweden, #17089102). For DREADD experiments ...
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bioRxiv - Microbiology 2019Quote: ... cells were seeded in 12-well plates at 180,000 cells/well in the presence of Dharmafect IV reagent (Dharmacon, GE Healthcare) and 12.5-25 nM of siRNA duplexes or 100 nM mimic miR ...
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bioRxiv - Cell Biology 2019Quote: ... Quantification of total and individual subline cell numbers was performed either using custom protocols in Developer Toolbox 1.7 software (GE Healthcare) or CellProfiler (Carpenter et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF7 and HT29 cells expressing H2B-GFP were treated as indicated and imaged on a DeltaVision microscope (Applied Precision Ltd.). Interphase phenotypes were analyzed by quantifying nuclear morphology ...
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bioRxiv - Immunology 2019Quote: ... or neutrophils were isolated by dextran (250,000) sedimentation of red blood cells (RBC) followed by Ficoll-Paque PLUS H-1077 (GE Healthcare) gradient separation as described before with modification (Denny et al. ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by means of density centrifugation with Ficoll-Paque Plus (GE Healthcare, Pittsburgh, PA) and cultured in AIM-V medium (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... The cell debris was removed by centrifugation at 14,000 rpm and the supernatant was loaded onto an IMAC column (GE Healthcare) charged with nickel ...
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bioRxiv - Microbiology 2020Quote: ... inoculum was removed and cells were overlaid with DMEM + 10% fetal bovine serum (GE Healthcare Life Science, Piscataway, NJ, USA). After 1 day ...
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bioRxiv - Cell Biology 2019Quote: U-2 OS FUCCI cells were grown on a 96-well glass bottom plates (Whatman, Cat# 7716-2370, GE Healthcare, UK ...
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bioRxiv - Cancer Biology 2021Quote: ... cells and purified according to manufacturer’s instructions using Ni-chelate agarose (Quiagen) or glutathione coupled Sepharose 4B beads (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were collected from blood samples (~ 2ml) at all time-points with Ficoll-Paque (GE Healthcare) centrifuge method.
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bioRxiv - Immunology 2020Quote: ... Then cells were washed 3 times with PBS and stained with 75 μM CypHer5E NHS ester (GE Healthcare, PA #15401) for 1 hour at 25°C[13] ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... The suspension was centrifuged for 15 minutes at 4°C and 15,000 g to remove cell debris and the clear supernatant was applied to a PD-10 desalting column (GE healthcare) to remove excess free biotin using the gravity protocol according to the manufacturer’s instructions ...