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Citations for GE Life Sciences :
5501 - 5550 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNC) were extracted from fresh BM using Ficoll-Paque Plus (GE Healthcare, #17-1440-02). Bone marrow MNCs were also extracted from two patients (ALL10 and ALL12 ...
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bioRxiv - Cancer Biology 2020Quote: ... All breast cancer cell lines were maintained in Dulbecco’s Modification of Eagle’s Medium (DMEM, GE Life Sciences) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Fresh peripheral blood mononuclear cells (PBMCs) were obtained from buffy coats by Ficoll Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells were obtained by Ficoll gradient separation on Ficoll-Paque (GE Healthcare, Amersham, UK). Then ...
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bioRxiv - Microbiology 2020Quote: ... Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficoll-paque plus (GE Healthcare), the serially diluted GA-inactivated SARS-CoV-2 samples were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... images were acquired on a IN Cell 2200 automated high-throughput fluorescence microscope (GE Healthcare, Chicago, USA) using IN Cell Analyzer (version 6.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... while HCT-15 and HL-60 cells were grown in RPMI medium (Hyclone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (TianJin Haoyang Biological Products Technology Co. ...
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bioRxiv - Genomics 2021Quote: ... Btbd7- and Zcchc8-knockout SIMS cells were maintained in phenol red-free DMEM (GE Healthcare/Cytiva, SH30284.01) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2022Quote: Cell extract samples were separated on 12% SDS-PAGE gels before transfer to nitrocellulose membranes (GE Healthcare). Membranes were blocked in PBS containing 5% milk before probing with primary antibodies at concentrations listed in S2 Table ...
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bioRxiv - Cancer Biology 2022Quote: 293T cells were cultured in normal DMEM media supplemented with 10% bovine growth serum (GE Healthcare, SH30541.03) and 1% penicillin-streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were further purified by centrifugation over a Percoll gradient (GE Healthcare, 17-0891-01, Sigma Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from primary donors using Ficoll-Paque Plus (GE Life Sciences) and frozen until use ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were separated from myelin and debris by 30% isotonic percoll gradient (#17-0891-01, GE Healthcare) in Myelin Gradient Buffer (MGB) ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated using the density gradient centrifugation method (GE Healthcare, STEMCELL Technology). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The immune cells were obtained by density gradient centrifugation with Percoll (17-0891-09, GE Healthcare, USA) according to the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... and the peripheral blood mononuclear cells (PBMCs) isolated by Ficoll-Paque gradient (GE Healthcare, Boston, MA, USA). Informed consent was obtained in accordance with the Helsinki Declaration and the Review Board of the Dana-Farber Cancer Institute.
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bioRxiv - Cell Biology 2022Quote: ... cells were spotted onto slides and images were acquired using a Deltavision Elite microscope (GE Healthcare Biosciences) with a 60X oil immersion objective ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of cell lysate was purified over Glutathione-Sepharose 4B (GS4B) (GE Healthcare, Boston, MA, USA) or Ni-NTA resins (Qiagen) ...
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bioRxiv - Pathology 2020Quote: ... We also prepared red blood cells from EDTA-treated swine blood by using Percoll (GE Healthcare, USA) and kept it in an RPMI 1640 medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation (Ficoll-Hypaque, GE Healthcare life sciences) from buffy coats from healthy donors (Sanquin Amsterdam ...
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bioRxiv - Molecular Biology 2019Quote: Primary CD4+ T cells were isolated from buffy coats from healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCells Technologies) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated using 8-12% polyacrylamide gels and transferred to Amersham Nitrocellulose Membranes (GE healthcare) via Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... hematopoietic cells were enriched by density gradient centrifugation with 40/90 Percoll gradient (GE Healthcare Life Sciences) for 30 min at 2,500 rpm ...
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bioRxiv - Genomics 2019Quote: ... Mononuclear cells (MNCs) were isolated by density gradient separation (Ficol-Paque, GE Healthcare Life Sciences, Pittsburgh, PA) and frozen for later use ...
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bioRxiv - Cell Biology 2020Quote: We performed the quantitative evaluation of surface area of cardiomyocytes by using IN Cell Analyzer (GE healthcare). αActinin positive cardiomyocytes with nuclei (Hoechst ...
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bioRxiv - Immunology 2020Quote: ... The resuspended cells were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The decidual leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
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bioRxiv - Biophysics 2020Quote: ... and the clarified cell extract was loaded onto a 5-mL HisTrap HP column (GE Life Sciences) equilibrated in HisTrap Buffer A ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated from the cells using an Illumina RNAspin Mini RNA isolation kit (GE Healthcare). The samples were treated with DNase I to remove any contaminating genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Ficoll-Paque-PLUS (GE Healthcare 17144002). CD14+ monocytes were purified using MojoSort™ Human CD14 Nanobeads (Biolegend 480093 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences). To slow Fe2+-oxidation and precipitation in equilibration buffer ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from the cells using an Illumina RNAspin Mini RNA isolation kit (GE Healthcare). The samples were treated with DNase I to remove any contaminating genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood by Ficoll-Paque (GE Healthcare, ThermoFisher) density gradient centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... MIN6 cells were grown in optimized DMEM (AddexBioTechnologies) supplemented with 10% FBS (HyClone, GE Healthcare Life Sciences), 50 μM 2-β-mercaptoethanol (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE001 was purified from cell supernatants after 5 days of induction using Nickel Excel resin (GE Healthcare), followed by size exclusion chromatography with a Sephadex S200 column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells were isolated from whole blood samples using Ficoll-paqueTM (GE Healthcare Life Sciences), with subsequent RNA and DNA isolation as detailed in Vazquez Bernat et al ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was removed by resuspending pelleted cells into 30% isotonic Percoll (GE Healthcare, Cat#17-5445-02) and centrifuging at 300g for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were freshly isolated from heparinized blood using Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation.
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bioRxiv - Molecular Biology 2023Quote: ... Live-cell imaging was performed on a Deltavision Elite system using a 40× oil objective (GE Healthcare). Images were acquired every 10 or 15 min for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted using the Illustra RNAspin kit with DNaseI treatment (GE Healthcare). RT-PCR was performed with 100 ng of total RNA using SuperScript III One-Step RT-PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and PC3M cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone, GE Healthcare Life Sciences, Logan, UT) supplemented with 10% fetal bovine serum (HyClone ...
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bioRxiv - Biochemistry 2024Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Imaging pads were stained with 8 µL of dye mix (25 µg/mL DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... All other cell lines were grown in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences, Logan, UT) with 10% fetal bovine serum (10% FBS–DMEM) ...
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bioRxiv - Genetics 2023Quote: ... Cells were mounted on a slide and imaged using DeltaVision Elite wide-field fluorescence microscope (GE Healthcare) with a 60x/1.516 oil immersion objective ...
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bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protan, GE Healthcare). Incubation with specific antibodies was followed by peroxidase-conjugated secondary antibodies and detection using SignalFire ECL Reagent (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and cell pellets were re-suspended in 37% isotonic Percoll (GE Healthcare, IL, USA) at room temperature ...