Labshake search
Citations for Quantifoil Micro Tools :
251 - 300 of 607 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
-
bioRxiv - Biophysics 2023Quote: ... 3.5 μl of concentrated purified protein was applied to glow-discharged copper holey carbon grids (Quantifoil R 1.2/1.3, 300 mesh). In all cases ...
-
bioRxiv - Biochemistry 2020Quote: ... the grids were prepared by applying 3 μl sample on glow-discharged holey carbon grids (Quantifoil Mo 2/2 200 mesh). The sample was ice-embedded using a LEICA EM GP2 (Leica Microsystems ...
-
bioRxiv - Microbiology 2023Quote: CryoEM samples were prepared by applying 5 μL aliquots of purified PLE PLPs to R2/2 Quantifoil grids and R2/2 Quantifoil grids coated with 2nm ultrathin Carbon (QUANTIFOIL®) that had been glow discharged for 45 seconds in a Pelco Easiglow glow discharging unit ...
-
bioRxiv - Biochemistry 2024Quote: ... of the freshly eluted complex peak solution (4.0 mg/mL) was loaded onto glow-discharged holey carbon grids (Quantifoil 2/2). The procedures for blotting and plunge freezing the grids were the same as above ...
-
bioRxiv - Cell Biology 2022Quote: ... Thin sections of 50μm were obtained using a vibratome and placed onto a glow discharged electron microscopy grid (UltrAuFoil, 200 Au mesh, 2/2 Au film (Quantifoil Micro Tools)) that had been pre-clipped into Autogrids (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... A flat-sided planchette was placed flat side upwards and a glow discharged (Glocube, Quorum, Lewes, UK) electron microscopy grids (UltraAuFoil, 200 Au mesh, 2/2 Au film (Quantifoil Micro Tools) placed on top ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 μL of purified AVP-V2R-Gs-Nb35 at 0.75 mg/ml concentration were applied on glow-discharged Quantifoil R 1.2/1.3 300 mesh copper holey-carbon grids (Quantifoil Micro tools GmbH, Germany), blotted for 4.5s and then flash-frozen in liquid ethane using the semi-automated plunge freezing device Vitrobot Mark IV (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: Tfam+/− MEFs (TFAM Het MEF) and SV40 mouse embryonic fibroblasts (SV40 MEF) were cultured on R ¼ Carbon 200-mesh gold electron microscopy grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture using a Vitrobot Mark 4 (Thermo Fischer Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
-
bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
-
bioRxiv - Biochemistry 2024Quote: Samples were prepared by applying the polymerized R-β-Actin actin to freshly glow-discharged Quantifoil 3.5/1 on 200 mesh copper grids (Quantifoil GmbH, Germany), using a Leica GP2 (Leica Microsystems GmbH ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 µL of proteins / proteins-phospholipids mix samples were gently deposited on glow discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
-
bioRxiv - Microbiology 2020Quote: ... loaded onto glow-discharged carbon grids (R2/2, Quantifoil) and plunge-frozen into liquid ethane-propane mix cooled at liquid nitrogen temperatures with a Mark IV Vitrobot(Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: EM grids (G300F1, R2/2 Quantifoil holey carbon, gold) were glow-discharged and treated with bovine fibronectin (20 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... loaded onto glow-discharged carbon grids (R2/2, Quantifoil) and plunge-frozen into liquid ethane-propane mix cooled at liquid nitrogen temperatures with a Mark IV Vitrobot maintained at room temperature and 70% humidity ...
-
bioRxiv - Microbiology 2020Quote: ... loaded onto glow-discharged carbon grids (R2/2, Quantifoil) and plunge-frozen into liquid ethane-propane mix cooled at liquid nitrogen temperatures with a FEI Mark IV Vitrobot maintained at room temperature and 70% humidity ...
-
bioRxiv - Cell Biology 2021Quote: Carbon-coated gold TEM grids (Quantifoil NH2A R2/2) were glow-discharged at 2 mA and 1.5–1.8 × 10-1 m bar for 1 min in an ELMO (Cordouan ...
-
bioRxiv - Biochemistry 2022Quote: UltrAuFoil on gold 200 mesh R2/2 grids (Quantifoil) were subjected to micropatterning using the Primo module from Alveole mounted on a Leica DMi8 microscope following the manufacturer procedure ...
-
bioRxiv - Microbiology 2022Quote: ... loaded onto glow discharged carbon grids (R2/2, Quantifoil) and plunge-frozen using a Vitrobot Mark IV (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... Quantifoil Cu 200 R2/2 holey carbon grids (Quantifoil) were glow discharged negatively for 45 sec before sample application ...
-
bioRxiv - Biophysics 2024Quote: ... Holey carbon grids (Quantifoil Cu 300 mesh, R2/2) were prepared with fiducials by glow-discharging ...
-
bioRxiv - Cell Biology 2023Quote: Carbon-coated gold TEM grids (Quantifoil NH2A R2/2) were glow-discharged at 2 mA and 1.5– × 10−1 m bar for 1 minute in an ELMO (Cordouan ...
-
bioRxiv - Immunology 2024Quote: ... a 3-μl aliquot was applied onto a glow-discharged QUANTIFOIL® R 1.2/1.3 on Cu 300 mesh grid + 2 nm C Holey Carbon Films with ∼ 2 nm continuous carbon on top (QUANTIFOIL, #C2-C14nCu30-50). The grids were blotted for 2.0 s in 100% humidity at 6°C and plunged into liquid ethane using a VitrobotMkIV (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2021Quote: ... with 12-nm-thick holey R1/4 and R1.2/20 SiO2 film (Quantifoil) were used for the experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh grid, SiO2, Quantifoil Micro Tools) were glow-discharged and UV irradiated for 30 min for sterilization before being immersed in cell culture medium in 35-mm IBIDI μ-Dish ...
-
bioRxiv - Synthetic Biology 2023Quote: 4 µl of sample was adsorbed onto holey carbon-coated grid (Quantifoil, Germany) that was glow-discharged ...
-
bioRxiv - Neuroscience 2024Quote: ... gold mesh grids with holey R1/4 SiO2 film (Quantifoil Micro Tools GmbH) were glow discharged for 60s at 15 mA using an EasiGlow system ...
-
bioRxiv - Cell Biology 2024Quote: 4 µl of yeast cells were pipetted on Cu R2/1 grids (Quantifoil), blotted manually from the backside of the grid for ∼4 s and plunge frozen in liquid ethane using a manual plunger ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... were applied to glow-discharged (25 mA, 10 s) QuantiFoil Gold R 0.6/1 300-mesh holey carbon grids (QuantiFoil, Micro Tools GmbH, Germany), blotted for 4.5 s ...
-
bioRxiv - Cell Biology 2022Quote: The IL-11Δ10/IL-11RαD1-D3/gp130EC complex (1.5 mg/mL) in the presence of 1-2 mM n-dodecyl β-D-maltoside was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded onto gold grids (either ‘UltrAuFoil’ 200 Au mesh, R2/2 gold film, or Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools, Großlöbichau, Germany) and grown to 50 % confluency before plunge freezing ...
-
bioRxiv - Biophysics 2023Quote: ... 0.5 μL L-PEG-L (8 mg/ml) and 0.5 μL Pd(NO3)2 (1 mg/ml) was applied to holey carbon grid (Quantifoil 200 mesh Cu R2/2) which was glow discharged 45 s at 15 mA ...
-
bioRxiv - Biochemistry 2023Quote: ... An aliquot (2 μL) of this polarized vesicle solution was applied onto a glow-discharged holey gold grid (Quantifoil Au R2/2, 400 mesh). After incubating the sample on the grid for 3 minutes at 10 °C with a humidity of 100% ...
-
bioRxiv - Microbiology 2020Quote: ... Quantifoil Cu R2/2 Finder grids (Quantifoil Micro Tools GmbH) were glow-discharged at 15mA for 1min ...
-
bioRxiv - Biophysics 2020Quote: UltrAuFoil R2/2 200 mesh TEM grids (Quantifoil Micro Tools) were prepared by 200 second glow discharge in 0.39 mBar of room air using a Pelco easiGlow (Ted Pella) ...
-
bioRxiv - Molecular Biology 2020Quote: Quantifoil R2/2 holey carbon copper grids (Quantifoil Micro Tool) were prepared by first applying an additional thin layer of continuous carbon and then glow-discharging them for 15 sec at 15 mA using an easiGlow Discharge cleaning system (PELCO) ...
-
bioRxiv - Biochemistry 2021Quote: Quantifoil R2/2 holey carbon copper grids (Quantifoil Micro Tool) were prepared by applying an additional thin layer of continuous carbon ...
-
bioRxiv - Molecular Biology 2022Quote: ... either carbon on Cu/Rh (Quantifoil R2/2 200 mesh) or gold on gold UltrAuFoil R1.2/1.3 300 mesh ...
-
bioRxiv - Biochemistry 2022Quote: Standard holey carbon grids (Quantifoil, Cu 200 mesh, R2/2) were glow discharged for 30 s at 15 mA on the negative setting ...
-
bioRxiv - Immunology 2024Quote: Quantifoil R2/2 holey carbon copper grids (Quantifoil, Grosslöbichau, Germany) were cleaned by overnight incubation in ethyl acetate and coated with 1 nm continuous carbon foil by floatation ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μL of the ATP13A2 sample were applied to a glow-discharged (PELCO easiGlow; 0.39 mBar, 25–30 mA, 40–45 s) gold holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh). The grid was blotted for 3–4 s and plunge-frozen in liquid-nitrogen-cooled liquid ethane using Vitrobot Mark IV (FEI ...
-
bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
-
bioRxiv - Immunology 2020Quote: ... 3 μL of the complex was mixed with 1 μL of a n-Dodecyl-β-D-Maltopyranoside (DDM) solution to a final DDM concentration of 0.06 mM and applied to a grid (Quantifoil R 1.2/1.3, 400), which had been plasma-cleaned for 5 seconds using a mixture of N2/O2 (Gatan Solarus 950 Plasma system) ...
-
bioRxiv - Biophysics 2024Quote: ... Four μl of this mixture was deposited to glow-discharged cryo-EM grids (UltrAuFoil R1.2/1.3, Quantifoil Cu 2/2, or Quantifoil AU 100 Holey carbon Films R2/1), blotted for 3 seconds at relative humidity 98% and temperature 4°C and plunge-frozen into liquid ethane (Vitrobot) ...
-
bioRxiv - Biophysics 2020Quote: ... 4 µl sample was applied to each freshly glow-discharged grid (Quantifoil, R1.2/1.3). The grids were then plunge-frozen in liquid ethane ...
-
bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
-
bioRxiv - Biochemistry 2024Quote: IN-IBD complex (0.1 mg/mL) was applied onto freshly plasma cleaned (20s, Solarus plasma cleaner) holey grids (Quantifoil UltrAufoil R 1.2/1.3 300 mesh) at 4°C in the cold room with ∼90% humidity ...
-
bioRxiv - Biophysics 2024Quote: ... to produce a fiducial marker along with 0.01% NP-40 and 10 μM tubulin solution with BRB80 buffer and 1 mM GTP before the solution was dropped onto the grids (QUANTIFOIL R 1.2/1.3 Cu 200). The grids were blotted with vitrobot (Thermo Fisher Scientific ...