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Citations for Quantifoil Micro Tools :
51 - 100 of 607 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: QUANTIFOIL R 2/1 grids with 2nm carbon layer (Cu 200, Quantifoil Micro Tools) were glow discharged with 25 mA with negative polarity for 60 seconds in a PELCO easiGlow glow discharge unit ...
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bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... 10 µl sample was applied onto a glow discharged copper grid coated with holey carbon (R 2/2; Quantifoil, Jena, Germany), and subsequently dipped onto 500 µl deionized H2O for 1 second to clear the sucrose ...
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bioRxiv - Biophysics 2024Quote: ... samples that have been analyzed here are Influenza A/Hong Kong/1/1968 virions that are produced from MDCK cells grown directly on cryoEM grids (QF AU200 R 2/2, Quantifoil, Germany), infected at a multiplicity of infection <1 ...
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bioRxiv - Neuroscience 2022Quote: Holey copper or molybdenum EM grids (Quantifoil R 2/1 200 mesh; Quantifoil, Jena, Germany) were glow discharged (Harrick Plasma cleaner PDC-3XG ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL were applied to freshly glow-discharged R 2/1 holey carbon grids (Quantifoil) at 4 °C and 95% humidity ...
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample was applied on glow-discharged Cu holey carbon grids (Quantifoil R 1.2/1.3) and incubated for 1 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... before applying 4 µL of the solution to a glow-discharged (30s at 35 mA) copper R 2/2 200-mesh holey carbon grid (Quantifoil Micro Tools). The grids were blotted manually from the back side with Whatman filter paper #1 for 2-4 s ...
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bioRxiv - Molecular Biology 2023Quote: ... and samples for cryo-SPA were deposited onto 200-mesh holey carbon grids (Quantifoil Micro Tools, R 2/2 X-103-Cu200) which were first glow-discharged for 2.5 min using an ELMO glow discharge unit (Cordouan Technologies) ...
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bioRxiv - Biochemistry 2024Quote: Three microliters of liposomes or proteoliposomes were applied to glow discharged Quantifoil R 2/2 grids (Quantifoil Micro Tools GmbH, Jena, Germany) or Lacey grid (Ted Pella inc.) ...
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bioRxiv - Biophysics 2024Quote: ... 2-4 chameleon self-wicking grids (Quantifoil Active 300 mesh; Quantifoil) were loaded into the instrument and glow discharged for 40-45 seconds at 12 mA ...
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bioRxiv - Biophysics 2024Quote: ... 2-4 chameleon self-wicking grids (Quantifoil Active 300 mesh; Quantifoil) were loaded into the instrument and glow discharged for 40-45 seconds at 12 mA ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto plasma-cleaned Quantifoil R 2/1 (Cu, 400-mesh; Quantifoil Micro Tools GmbH) grids ...
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bioRxiv - Cell Biology 2022Quote: Vero-E6 were seeded on holey carbon film R 5/20 gold 200 mesh EM grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2020Quote: The cell suspension in sea water was seeded onto R 2/2 (SiO2) on Au G200F1 finder grids (Quantifoil Micro Tools GmbH, Germany) and incubated with filtered fresh sea water at 18 °C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Qualified nucleosomes were then loaded in a freshly glow discharged holey carbon golden grid (Quantifoil™ R 2/2 on 300 gold mesh) (1.0-2.5 µg of nucleosomes were diluted in 40 mM NaCl reconstitution buffer in 3 μL) ...
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bioRxiv - Molecular Biology 2023Quote: ... The complex was incubated at RT for 20 mins and pipetted onto glowed discharged cryo-EM grids (Quantifoil R 2/2 Au 300). Grids were frozen in liquid ethane by using a Vitrobot Mark IV (ThermoFischer ...
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bioRxiv - Biophysics 2024Quote: ... 4 × 104 MDM were seeded on glow discharged and UV-light sterilized 200-mesh EM gold grids coated with R 2/2 holey carbon films (Quantifoil Micro Tool GmbH), which were placed in a glass-bottomed ‘microwell’ of 35-mm MatTek dish (MatTek ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biophysics 2024Quote: ... 2 μL of 2 μM taxol-microtubules were incubated on a glow-discharged holey carbon cryo-EM grid (QuantiFoil, Au 300 R 1.2/1.3) for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... and seeded onto a glow discharged holey carbon covered EM-grids (R 2/1, Au 200, Quantifoil). After approximately 16 h ...
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bioRxiv - Biophysics 2024Quote: ... 10 mM DTT was incubated for 3 days at 30 °C and 2.5 μL of the solution was applied onto a Quantifoil R 1.2/1.3 200 mesh Cu grid (Quantifoil) and plunge-frozen in liquid ethane by a Vitrobot Mark IV (FEI) ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl sample was applied onto Au R2/2 200 mesh (Quantifoil) grids ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 μl diluted T33-549 (final concentration ∼0.9 mg/ml) was applied to the glow-discharged surface of grids (QUANTIFOIL® R 2/2 on Cu 300 mesh + 2 nm C), then immediately plunged into liquid ethane after blotting for 4s with a blot force of 0 ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2021Quote: ... of purified CtenDNAV-II virons (10 mg ml-1) was deposited onto freshly glow-discharged holey carbon-coated copper grids (Quantifoil R 2/2, 300 mesh, copper) followed by 2 s of blotting in 100% relative humidity for plunge-freezing (Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2023Quote: ... pristine graphene-coated R2/4 grids (Graphenea) were initially used for optimisation purposes but 2 nm formvar/amorphous carbon R2/2 grids (Quantifoil) were used for data collection ...
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bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2024Quote: 4 μl of the inhibition reaction mixture was applied onto plasma cleaned holey carbon grids coated with 2nm thick amorphous carbon (Quantifoil R 2/2, UT, 300 Mesh, Copper). Following 30 sec of incubation inside the chamber of FEI Vitrobot MK IV (FEI ...
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bioRxiv - Biophysics 2023Quote: R 1.2/1.3 (Quantifoil) (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... Those nucleosomes that passed the quality control were then loaded into a freshly glow discharged holey carbon golden grid (Quantifoil™ R 2/2 on 300 gold mesh) (2.5 µg of nucleosome diluted in 40 mM NaCl reconstitution buffer in 3 μl was loaded in the grid) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Molecular Biology 2020Quote: The grids were prepared by applying 4 µL of each complex at ∼70 nM to 400 mesh holey carbon Quantifoil 2/2 grids (Quantifoil Micro Tools). The grids were blotted for 1.5 sec at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl sample was applied onto Quantifoil grid Rh/Cu 200 R2/2 (Quantifoil, Inc), and was flash frozen into liquid ethane using a Vitrobot (FEI ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl sample was applied onto Quantifoil grid Cu/Rh 200 R2/2 (Quantifoil, Inc), and was flash frozen into liquid ethane using a Vitrobot (FEI ...
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bioRxiv - Biochemistry 2024Quote: ... Grids (Quantifoil UltrAuFoil R 0.6/1) were glow-discharged (20 mA ...
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bioRxiv - Cell Biology 2024Quote: EM support grids (Au grids 200 mesh, carbon or SiO2 foil, R2/2 or R1/4, Quantifoil) were glow discharged with a Pelco easiGlow for 90 sec at 15 mA ...