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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Antigen retrieval was performed for 30 min in citrate buffer (Sigma-Aldrich, St. Louis, MO, C9999, 1:10). Sections were incubated with 3% hydrogen peroxidase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Neuroscience 2024Quote: ... the retina was first treated with pre-heated (about 70 °C) 1× antigen retrieval citrate buffer (Sigma, C9999), then followed the same protocol as with the brain section described above ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of cell lysates were incubated with Flag-M2 (M8823, Sigma) or GFP (gtma ...
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bioRxiv - Molecular Biology 2019Quote: ... Tissue lysates were mixed with 1-bromo-3-chloropropane (B9673, Sigma-Aldrich) at a ratio of 1:4 and the RNA containing aqueous/organic phase was separated using 5Prime PhaseLock Gel Heavy (#2302830 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysate was then acidified to 1 % trifluoroacetic acid (Sigma Cat. No. 302031) for DNA hydrolysis ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Microbiology 2021Quote: ... and 10% formamide in DEPC-treated H2O) with anti-influenza A virus NP antibody (Millipore, 1:2,000) and FISH probes ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were incubated O/N with prewashed 1:1 anti-HA agarose beads (Sigma, Cat# A2095) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Antigen retrieval performed with sodium citrate buffer (Sigma-Aldrich C9999) and a steamer ...
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bioRxiv - Microbiology 2020Quote: ... Antigens were emulsified in Freund’s Complete Adjuvant (Sigma-Aldrich, F5881) for the first immunization or Freund’s Incomplete Adjuvant (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigens were retrieved using 0.1% trypsin (Sigma-Aldrich, MO, USA) and non-specific staining was blocked using 10% fetal bovine serum/neonatal goat serum for 1 hr prior 90 min incubation with either biotinylated anti-laminin antibody (Novus Biologicals ...
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bioRxiv - Immunology 2022Quote: ... Antigens were retrieved using citrate buffer (pH 6.0, Sigma-Aldrich) in a KOS MicrowaveStation (Milestone SRL) ...
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bioRxiv - Cancer Biology 2022Quote: ... The tested antigens included Cardiolipin (50 μg/mL, Sigma C0563), KLH (5 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 10X Citrate Buffer (Sigma-Aldrich) in a capped coplin jar microwaved for ∼45 seconds followed by 15-minutes incubation in microwave ...
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed in 1x citrate buffer (Sigma, C9999) with a pressure cooker for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor proliferation in the R26-GB2/MMTV-PyMT animals was assessed by PCNA (proliferating cell nuclear antigen) immunostaining using the MAB424R antibody (1:10,000; clone p10, Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies conjugated to horseradish peroxidase (1:25,000; catalog # A0545, Sigma). Either photographic film or a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: Rehydrated histological sections underwent antigen retrieval by incubation with 1 mg/mL pepsin in 0.5 M acetic acid (Millipore) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lysates and concentrated particles were analyzed for FLAG (Sigma–Aldrich; A8592; 1:10,000), GAPDH (Sigma–Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... One tube of lysate was treated with 1 μL of trehalase (Sigma-Aldrich) enzyme or vehicle control ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected RIPA buffer containing 1 × protease inhibitor cocktail (P8340; Sigma-Aldrich). Twenty micrograms of protein per sample was loaded on polyacrylamide–SDS gels ...
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bioRxiv - Immunology 2021Quote: Bacterial cultures or cell lysates were acidified to pH=1 using HCl (Sigma) and extracted twice with ethyl acetate (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with 1 μg anti-FLAG-BioM2 antibody (#F9291, Sigma-Aldrich) or without antibody for 3 h at 4°C under constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were treated with 1 U/mL RNase T1 (Sigma-Aldrich, cat#R1003) for 15 minutes at 22 ℃ ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... Cryostat sections or whole embryos were stained using standard protocols and antibodies against the following antigens: HA (1:250, Sigma), β-catenin (1:300 ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed boiling in pH6 Citrate buffer (Sigma-Aldrich) for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was followed by permeabilization with 0.5% Triton-X100 (Sigma). EdU incorporation was followed by EdU Click-IT chemistry according to manufacturer’s instructions (ThermoFisher Scientific) ...