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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: 500 µl diluted lysate was incubated with the antibody against β-catenin (10 µg; Sigma, MA1-301, 1:2000) overnight at 4L°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... FDG is mixed in LB to 0.2 mM with IPTG (1 mM, final concentration), sodium N-lauroyl sarcosine solution (0.1%, cell lysate buffer to allow FDG in) (Sigma), Pluronic F127 (0.001%) ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of clarified lysate was incubated with 30 μl of pre-rinsed streptavidin magnetic particles (11641786001, Sigma-Aldrich) in 1 ml RIPA buffer for 16h ...
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Chromatin lysates were prepared in LB3 (50 mM Tris-HCl pH8.0, 1% SDS, 10 mM EDTA, proteinase inhibitor cocktail (Sigma)) ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml of the lysate was added to 40 μl of equilibrated ANTI-FLAG® M2 Affinity Gel (Sigma). The lysate was incubated with the affinity gel in the cyclomixer for 4 hours at 4 ⁰C ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was carried out with 0.2 U/mL Chondroitinase ABC (C2905, Sigma, Merck, Darmstadt, Germany) at 37 °C for two hours for DCN ...
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bioRxiv - Physiology 2020Quote: ... sections were dewaxed and rehydrated prior to enzymatic antigen retrieval (trypsin from porcine pancreas (T7168, Sigma)) ...
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bioRxiv - Neuroscience 2021Quote: ... Heat-mediated antigen retrieval was performed by placing slides in 1x sodium citrate buffer (PHR1416, Sigma), pH 6.0 ...
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bioRxiv - Immunology 2023Quote: ... Diphtheria antigen samples were prepared by desorption with sodium citrate (Sigma-Aldrich Chemie GmbH Taufkirchen, Germany) [23] ...
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bioRxiv - Immunology 2023Quote: ... TUNEL staining was conducted following antigen retrieval using the In Situ Cell Death Detection Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at in a microwave at circa 97°C
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated with an alcohol series and incubated in Tris-EDTA antigen retrieval buffer (#E1161, Sigma-Aldrich) for 20 minutes in a pressure cooker at 95°C ...
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bioRxiv - Physiology 2024Quote: ... and distilled water followed by heat-induced antigen retrieval in citrate buffer (cat# C9999, Sigma-Aldrich) using a 2100 Retriever (Electron Microscopy Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by submerging slides in boiling 10 mM sodium citrate buffer (Sigma-Aldrich), before washing with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...