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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Somatostatin visualization required an antigen retrieval process in citrate buffer (Sigma Aldrich C8532, pH 6.0) for 30 minutes prior to blocking ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Microbiology 2020Quote: ... followed by two boosts of 0.1 mg antigen mixed with Freund’s Incomplete Adjuvant (Sigma F5506). The resulting antibodies were used at 1:1,000 for western blots ...
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bioRxiv - Developmental Biology 2021Quote: ... After antigen retrieval the sections were permeabilized in 0.25% Triton X-100/PBS (Sigma-Aldrich) for 10 minutes followed by washing in PBS ...
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bioRxiv - Genomics 2021Quote: ... Antigen retrieval was done by microwaving for 6 minutes in citrate buffer pH 6 (Sigma). After quenching in 3% H2O2 in Methanol ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed by boiling in 10mM sodium citrate buffer pH 6.0 (Sigma Aldrich) in pressure cooker for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... and antigen retrieval with 0.01% sodium citrate buffer (pH 6.0) (Sigma-Aldrich, Cat# C9999-100ML). The sections were then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-proliferating cell nuclear antigen (PCNA) (RRID: AB_477413; Sigma, St. Louis, MO, USA, #P8825), rabbit anti-NeuN (RRID:AB_ 10807945 ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes and the antigen-antibody reaction was visualized using di-aminobenzidine (DAB, Sigma) as the chromogen ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue antigens were retrieved by boiling in sodium citrate solution (10 mM, pH 6.0, Sigma). For blocking unspecific antibody binding sites ...
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bioRxiv - Immunology 2023Quote: ... The pretreatment for antigen retrieval was incubation with 0.2% proteinase K (Sigma-Aldrich, Misuri, USA) in Tris buffer at 37°C for 8 min.
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed by heating the slides in 1x Citrate buffer pH 6 (Sigma) for 40 min ...
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bioRxiv - Cancer Biology 2023Quote: Immunofluorescence assays were performed after deparaffination and antigen retrieval in citrate buffer pH6.0 (Sigma Aldrich). The Click-iT AF488 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed using citrate buffer (Sigma-Aldrich, St. Louis, MO, USA, pH 6.0) for 30 min at 100 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer (100mM trisodium citrate (Sigma, Cat#S1804), and 0.5% Tween 20 (Thermo Fisher Cat#BP337) ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Bioengineering 2022Quote: ... Wild-type KP4 cells were transduced with concentrated virus and GFP-expressing cells were selected using puromycin selection (1 μg/mL, Sigma-Aldrich, St Louis, USA). GFP-expressing PDOs were created using the same virus as KP4 but with a slightly different transduction protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 10 % of each lysate was collected (input) and the remaining lysate was incubated with Anti-V5 Agarose Affinity Gel beads (Sigma) or Anti-FLAG M2 magnetic beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: GM130 immunoprecipitates from SV589 cell lysate or N74-strep pulldowns from cell lysate were treated with 5-50 µg/ml RNase A (Sigma). The RNase inhibitor RNasin Plus (Promega ...
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bioRxiv - Immunology 2021Quote: ... Lysates were incubated with FLAG M2 (Sigma, F3165) antibody and immunoprecipitated protein was run on a 4-12% Tris-Bis ...
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bioRxiv - Genetics 2021Quote: ... Cell lysate was incubated with anti-FLAG (Sigma) at 4 °C with gentle agitation overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysates were incubated with HA antibody (Sigma) for 16 h at 4 °C and subsequently Protein G beads (Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were then treated with RNase A (Sigma) at 37°C 1 hr in Lysis buffer 2 (Lysis buffer 1 with 0.5% Triton X-100 ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were immunoprecipitated using protein G (Sigma-Aldrich) beads coated with unconjugated anti-biotin antibody (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate concentration was estimated via Bradford Assay (Sigma) and a NanoDrop 2000 (Thermofisher).
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bioRxiv - Microbiology 2022Quote: ... Lysate was applied to an equilibrated HisTrap (Sigma) Ni-sepharose column and washed with 10mL wash Buffer A (50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were incubated with Ni-NTA beads (Novagen) for 3 h and washed with BC300 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with TALON resin (Novagen) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein lysates were incubated with Glutathione-Agarose (Sigma) at 4 °C for 2 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cell lysates were harvested in TRI Reagent (Sigma). Total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was treated with 9U/mL benzonase (Sigma) and clarified by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were treated with benzonase (707463, Novagen) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... In brief lysates were alkylated (iodoacetamide, Sigma, # I1149) for 4h at RT ...
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bioRxiv - Immunology 2023Quote: ... Lysates were treated with benzonase (Sigma, E1014- 25KU) and RNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the lysate was digested with DNase/RNase (Sigma) and treated with 2% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5% human platelet lysate (Sigma #SCM152) and 50 μg/ml Primocin (InvivoGen #ant-pm-2) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were treated with Benzonase Nuclease (Sigma-Aldrich) for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5% human platelet lysate (Sigma #SCM152), w/o antibiotics and exchanged to the plated cell medium ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was incubated with benzonase (Sigma-Aldrich) at 8°C for 20 min and then applied on a 5 ml MBP-trap column (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.