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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... lysates were incubated with anti-FLAG matrix (M8823, Millipore; pre-blocked with 1% BSA-PBS for 1h) for 2h ...
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bioRxiv - Genetics 2020Quote: ... The lysate was treated with Benzonase (Sigma) together with MgCl2 (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were treated with Benzonase (Sigma) together with MgCl2 (final concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates were applied to Glutathione-Agarose (Sigma), washed with 20 CV of resuspension buffer with 1% Tween-20 ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunohistochemical staining was performed on a Leica Bond III instrument using the following primary antibodies and Leica antigen retrieval conditions: involucrin (Sigma-Aldrich, catalog #I9018, 1:10,000), retrieval H1 (30min) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
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bioRxiv - Biochemistry 2020Quote: ... Cell lysate was injected into FPLC and run over two HisTrap FF Crude 1 mL columns (Sigma-Aldrich) with FPLC running buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates prepared from equal amounts of cells (between 100-165 OD600) were fixed with 1% formaldehyde (Sigma) for 15 min at RT ...
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bioRxiv - Molecular Biology 2019Quote: Cell lysates were generated with PBS/1% NP40 buffer supplemented with protease inhibitor cocktail (Sigma Cat. No. S8820). Protein sample loading buffer was added to cell lysates and proteins were separated on SDS-PAGE (BioRad Criterion TGX Precast Gel) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg of protein lysate was incubated overnight with 30 μL Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with agitation for 1 h at 4 °C with 250 IU of benzonase (#70746, Millipore) followed by 15 min centrifugation at 15,000 x g at 4°C to remove the insoluble fraction of the lysate ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were briefly sonicated using a M220 focused ultrasonicator and incubated with Myc antibody (1:500; Sigma, C3956) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... were primed with antigen emulsified in an equal volume of complete Freund’s adjuvant (Millipore-Sigma). The mice were boosted 2 and 4 weeks later with antigen emulsified in incomplete Freund’s adjuvant ...
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bioRxiv - Microbiology 2020Quote: ... were primed with antigen emulsified in an equal volume of complete Freund’s adjuvant (Millipore-Sigma). The mice were boosted 2 and 4 weeks later with antigen emulsified in incomplete Freund’s adjuvant ...
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bioRxiv - Neuroscience 2020Quote: ... heat-induced antigen retrieval was done using citrate buffer (10 mM citric acid (Sigma Aldrich), with 0.05 % Tween-20 (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer (100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer (100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min and antigen retrieval in Tris-EDTA buffer/PBS with 0.05% Tween (Sigma) for 5 min at 121 °C in the autoclave ...
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bioRxiv - Neuroscience 2021Quote: Antigen retrieval was performed using a pH 6.0 citrate buffer (Sigma-Aldrich, St. Louis, MO) by microwaving in a 1:1000 solution in DDW for 30 minutes ...