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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Herpes Simplex Virus 1 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Zoology 2019Quote: ... The zygotes were cultured in potassium-supplemented simplex optimised medium (KSOM, EMD Millipore) at 37°C in 5% CO2 incubator and then injected using a microinjector setup (XenoWorks Micromanipulator and Digital Microinjector by Shutter Instruments) ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... and K-modified simplex optimized medium (KSOM) were purchased from Sigma (St. Louis, MO, USA). Paraformaldehyde (PFA) ...
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bioRxiv - Bioengineering 2020Quote: ... Flavivirus Group Antigen (Millipore Sigma, MAB10216, 1:200), GABA (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Developmental Biology 2022Quote: ... The fertilized embryos were washed several drops of potassium simplex optimization medium (KSOM; Millipore, MR-106-D) and cultured in the same medium to the blastocyst stage at 37 °C under Ovoil™ (Vitrolife ...
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bioRxiv - Immunology 2023Quote: ... OVA antigen (Sigma) was dissolved in saline to a 1 mg/mL concentration ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Neural/Glial antigen 2 (NG2 - Millipore AB5320, 1:100); anti-PDGFRβ (R&D AF385 ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-flavivirus group antigen (1:800, MAB10216, Merck Millipore) and goat anti-Iba1 (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... neural/glial antigen 2 (NG2; OPCs; Millipore; AB5320; 1:200), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2024Quote: ... a human nuclear-specific antigen [(EMD Millipore; MAB1281 (1:1000)] ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed 1X antigen retriever solution (Sigma, pH 6.0). Tissues were incubated with primary antibody in 5% donkey serum ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Developmental Biology 2019Quote: Embryos at all stages were cultured on 35mm Petri dishes (Falcon) within microdrops of 10-15µl of Potassium Simplex Optimized Media with amino acids (KSOM-AA, Millipore), at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse-anti-flavivirus group antigen (Millipore: MAB10216; RRID: AB_827205; 1:500), pan-flavivirus hyperimmune mouse acites fluid (HMAF ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse monoclonal antibody against the FLAG antigen (F1804, 1:2,000; Sigma), or with polyclonal antibody against β-tubulin (PA1-41331 ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-neuronal nuclear antigen (NeuN, mouse: 1:200, Millipore, #MAB3771). The number and morphology of microglia in the hippocampi were assessed by staining for ionized calcium-binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2019Quote: ... virus dilutions were overlaid with 1 ml of Opti-MEM [1% Carboxymethylcellulose (Sigma), 2% FBS ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... All live embryos were cultured in 10μl microdroplets of KSOM (potassium Simplex Optimized Medium; (Lawitts and Biggers, 1991)) with a mineral oil (Sigma, M8410) overlay inside an incubator (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The fertilized eggs were transferred to 5 μL droplets of potassium simplex optimization medium with amino acids (KSOMaa) containing 0.00025% polyvinyl alcohol (P8136-250G; Sigma-Aldrich) and 100 μM ethylenediaminetetraacetic acid on a glass-bottomed dish and placed in an incubation chamber (Tokai Hit ...
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bioRxiv - Neuroscience 2021Quote: ... antigen retrieval was performed in 1% citrate buffer (Sigma-Aldrich, Madrid, Spain) and 0.05% of Tween 20 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit polyclonal antibody against the human ZCCHC10 antigen (HPA038944, 1:2,000; Sigma), mouse monoclonal antibody against the FLAG antigen (F1804 ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed in 1% citrate buffer (Sigma-Aldrich, Madrid, Spain) and 0.05% of Tween 20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed with pH 6.0 citrate buffer antigen retrieval solution (Sigma #C9999) in a pressure cooker and cooled for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Microbiology 2021Quote: ... influenza virus NP (clones A1 and A3, MAB8251, Sigma-Aldrich, 1:100) and anti-mouse IgG AlexaFluor-488 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen Retriever (Sigma, Cat. No. C9999). The Vectastain Elite ABC Universal kit peroxidase (Horse Anti-Mouse/Rabbit IgG ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti adenomatous polyposis coli antigen (APC; clone CC1; Millipore, OP80-100UG; 1:100). Secondary antibodies were FITC conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... and neuronal nuclear antigen (NeuN, mouse anti-NeuN, 1:200, Millipore, MAB377, RRID: AB_2298772) and secondary Abs were added to slides during day1 and day2 of ISH ...
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bioRxiv - Neuroscience 2023Quote: ... and NG2 (neuron-glial antigen 2, AB5320, Merck Millipore, Burlington, MA, USA, 1:400) were used.
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies and antigen retrieval method used were anti-CLCa (Sigma, 1:200, citrate), anti-CLCb (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... anti-flavivirus group antigen antibody (clone D1-4G2-4-15, Sigma-Aldrich, 1:300), anti-dsRNA (SCICONS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysate was supplemented with 1 mM PMSF (Sigma). Proteins were then purified via a three strep purification protocol ...
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... anti flavivirus group antigen antibody (Millipore, MAB10216). The blots were developed by HRP reaction and imaged with a ProteinSimple FluorChem imaging system.
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bioRxiv - Cell Biology 2019Quote: ... Antigens were retrieved with 0.05 % pronase (Sigma). Sections were blocked with 3 % (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Antibodies against Lewis antigens were from Sigma and Santa Cruz biotechnology ...