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Citations for Millipore Sigma :
201 - 250 of 2250 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Input was diluted with 2X sample buffer (Sigma).
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bioRxiv - Immunology 2022Quote: ... Input was diluted with 2X sample buffer (Sigma). For mass spectrometry ...
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bioRxiv - Immunology 2022Quote: ... Input was diluted with 2X sample buffer (Sigma).
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bioRxiv - Microbiology 2024Quote: ... using KOD Polymerase Hot Start 2x Mastermix (Sigma). This amplification was performed with 20 cycles total ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x saline-sodium citrate (SSC; #S6639, Sigma-Aldrich), 1 µg/µl BSA (#R396A ...
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bioRxiv - Bioengineering 2024Quote: ... in 2x saline-sodium citrate (SSC) (Sigma-Aldrich) buffer ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were subsequently dehydrated 2x 5 min in 99% EtOH and 2x 5 min in Xylene prior to coverslip mounting using DPX (Sigma-Aldrich). All images were acquired on an Axio Scan.Z1 (Zeiss ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was started by addition of 25 μL ZDHHC master mix containing 20 nM ZDHHC20 and 2 μL of α-ketoglutarate dehydrogenase (KDH, Sigma-Aldrich, K1502). In the first reaction step ...
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bioRxiv - Genomics 2023Quote: ... Trachea was removed and the tissue was immediately injected with an enzyme mix based on previous work76 (Dispase II 50 caseinolytic u/ml (Sigma-Aldrich, D4693), Elastase 4.3 u/ml (Worthington Biochem ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the GCs and oocytes using TRI Reagents (Sigma-Aldrich). 1000 ng of total RNA from each sample was used for the preparation of cDNAs using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: Gonococcal (GC) broth was prepared by dissolving 3.75 g of protease peptone (Sigma-Aldrich), 0.25 g of potassium phosphate monobasic (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Monoclonal Anti-N-Cadherin/A-CAM (Clone GC-4, Product No. C2542, Sigma-Aldrich). Alexa Fluor secondary antibody (Goat anti-Rabbit IgG ...
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bioRxiv - Synthetic Biology 2024Quote: ... HPLC and LC-MS mobile phases were prepared using ≥99.0% (GC) dibutylamine (Sigma-Aldrich), 99.5+% 1,1,1,3,3,3-hexaflouro-2-propanol (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and conjugates were eluted in 2x Laemmli buffer (Sigma) at 70°C for 10 minutes prior to SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... and 2x complete ULTRA Protease Inhibitor Cocktail (Sigma Aldrich). Serum and BM supernatant IFNa and IFNγ levels were tested using the VeriKine kit (PBL Assay Sciences ...
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bioRxiv - Developmental Biology 2022Quote: ... 70% and 2x 99.7% in 1-Propanol(Sigma Cat.W292818):1xPBS (pH:9 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.014% (1X) or 0.028% (2X) tricaine (MS-222, Sigma) between 30 and 48 hpf ...
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bioRxiv - Biochemistry 2021Quote: ... a 2x molar excess of PHD (Phalloidin, Sigma Aldrich, freshly solved in methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 2X SIGMAFAST Protease Inhibitor Cocktail (Sigma-Aldrich, #S8830) and 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed twice with 2x SSC (Sigma-Aldrich) for 5 min then permeabilized with 0.2 M HCl + 0.7% Triton X-100 for 10 – 15 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were eluted in 2X Laemmli (Sigma-Aldrich) or deglycosylated by an 1h treatment with PNGase F (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM TCEP and 2X protease inhibitor cocktail (Sigma), using a Dounce homogeniser.
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM TCEP and 2X protease inhibitor cocktail (Sigma). To lyse the cells ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10% vol/vol foetal bovine serum (Hyclone FetalClone II Serum, Fischer Scientific, New Hampshire, US) and 21% vol/vol F12 nutrient mix (Sigma-Aldrich, Gillingham, UK), supplemented with 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was extracted from the GCs using TRI Reagent (Sigma-Aldrich, St. Louis, MO) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... strain II (MDCK-II)72 was purchased from Sigma Aldrich. Both cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... dispase II (Sigma) and papain (Worthington) ...
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bioRxiv - Neuroscience 2020Quote: ... II (Sigma Aldrich). The lysates were centrifuged at 16,000xg at 4°C for 30min ...
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bioRxiv - Physiology 2021Quote: ... Dispase II (Sigma) 0,5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... DNase II (Sigma) was added to a final concentration of 750 µg/mL ...
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bioRxiv - Biophysics 2019Quote: ... Myosin II (Sigma), KIF20A (Tocris bioscience) ...
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bioRxiv - Neuroscience 2022Quote: Deltorphin II (Sigma), naltrindole (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... dispase II (Sigma) and DNase I (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and collagenase (Worthington Biochemical Corp.)/dispase II (Sigma), and mechanically dissociated by trituration before being plated onto poly-D lysine-coated coverslips and incubated in Neurobasal™ media (Gibco 21103-049 ...
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bioRxiv - Microbiology 2021Quote: ... The samples were mixed with 2x Laemmli Buffer (Sigma-Aldrich) and used in SDS-PAGE electrophoresis with 12.5% polyacrylamide gels ...
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bioRxiv - Cell Biology 2019Quote: ... beads were resuspended in 2x Laemmli sample buffer (Sigma Aldrich), boiled for 5 min and stored at −20°C ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.6μg/ml (2x) Laminin (Mouse purified, Merck Millipore CC095). The agarose solution was mixed 1:1 with the cells/fibrinogen/laminin in 2i solution and kept at 37°C briefly to avoid polymerisation ...
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bioRxiv - Developmental Biology 2020Quote: ... 2x SSC and 10% (w/v) dextran sulfate (Sigma, D8906) in a humid chamber at 37 °C ...
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bioRxiv - Physiology 2021Quote: ... 2X Laemmli sample buffer was added (Sigma, catalog number S3401) at a 1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was mixed with 2X Laemmli Buffer (Sigma-Aldrich 53401), run on BioRad 4-20% precast SDS-polyacrylamide gels ...
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bioRxiv - Neuroscience 2022Quote: ... or frozen in 2x FM (Millipore Sigma; ES-002-10F). Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach ...
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bioRxiv - Immunology 2023Quote: Protein samples were denatured in 2x Laemmli buffer (Sigma-Aldrich) at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and/or phosphatase inhibitor cocktail (2x final concentration) (Sigma, 4906837001) were prepared on ice and subsequently incubated at 30 °C for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... Freshly extracted proteins were diluted in Laemmli buffer 2x (Sigma), incubated 10 min at 95°C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed with 2x SSC (Sigma-Aldrich, #335266) and incubated in a buffer of 2x SSC ...
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bioRxiv - Microbiology 2024Quote: ... 2x BHI was treated with Chelex 100 resin (Sigma-Aldrich) for 24 h at 4°C and supplemented with 40 µM EDDHA ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were loaded using Laemmli Buffer 2x (Sigma Aldrich) in equal mass (see above for the immunoblot procedure ...