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Citations for Millipore Sigma :
51 - 100 of 2250 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... PCRs were performed with the KOD Hot-start 2× master mix (Novagen), and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... A master mix was prepared of 0.2 mM Cy5-azide (Sigma-Aldrich) or Alexa Fluor™ 488 azide (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using 1 mL BugBuster Master Mix (Millipore #71456-4), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 6.25 μl 1x KAPA SYBR Fast master mix (Sigma Aldrich, Steinheim, Germany) and 0.208 μM forward and reverse primer ...
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bioRxiv - Bioengineering 2019Quote: ... the cell pellet was resuspended in the BugBuster master mix solution (Novagen) for protein extraction following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR master-mix used was: Taq polymerase (Novagen NovaTaq 0.04U/μL), primers (0.5 μM each) ...
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bioRxiv - Immunology 2022Quote: ... the master mix was filtered with Durapore 0.1µm PVDF membrane filter (Millipore) for 2 minutes at 100g before staining.
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bioRxiv - Systems Biology 2022Quote: ... qPCR assays were performed using FastStart Universal SYBR Green Master mix (Sigma) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were resuspended and lysed with BugBuster Master Mix (Sigma # 71456) using the recommended 5 mL per 1 g wet cell pellet ratio for 10 min at room temperature with gentle end-over-end rotation (10-15 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were gently lysed using the BugBuster Master Mix reagent (EMD Millipore) with 1 tablet per sample of cOmplete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were resuspended and lysed with BugBuster Master Mix (Sigma # 71456) using the recommended 5 mL per 1 g wet cell pellet ratio for 10 min at room temperature with gentle end-over-end rotation (10-15 rpm) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification was performed using KOD Hot Start Master Mix (Millipore Sigma) with primers listed in Supplementary Table 6 ...
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bioRxiv - Systems Biology 2023Quote: ... a master mix consisting of 0.2% DDM (D4641-500MG, Sigma Aldrich, Germany), 100mM TEAB ...
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bioRxiv - Biochemistry 2024Quote: ... 150nL of master mix (0.2% DDM (D4641-500MG, Sigma-Aldrich, USA/Germany), 100 mM TEAB (17902-500ML ...
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bioRxiv - Genetics 2023Quote: RNA samples were incubated overnight with a 2x nuclease mix containing 62.5 units of Benzonase (Sigma Aldrich), 5 units of Antarctic Phosphatase (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted cDNA was amplified by PCR using the Sybr Green Master Mix (Sigma), and raw threshold-cycle time (Ct ...
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bioRxiv - Bioengineering 2019Quote: PCR master mix: 1x KOD Xtreme Hot Start DNA Polymerase buffer (EMD Millipore), 1.2 µM (each ...
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bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...
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bioRxiv - Genetics 2019Quote: ... PCR plates were filled with a master mix for KOD Hotstart (Novagen #71086) PCR reactions containing Lister427 gDNA according to manufacturer’s specifications and primer pairs specific to each ORF were transferred into the associated well number for each plate by TECAN Freedom EVO 150 pipetting instrument ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 µL of the Roche FastStart Universal SYBR Green Master mix (Millipore Sigma) was mixed with cDNA and primers to achieve the final concentrations of 100 ng/µl and 400 nM ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (Sigma Aldrich) and run on the C1000 Touch™ thermal cycler (BIO-RAD ...
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bioRxiv - Bioengineering 2022Quote: ... Real-Time qPCR was performed using the Fast SYBR Green Master Mix (Sigma) in a QuantStudio 5 Real-Time-PCR-Cycler (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
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bioRxiv - Cell Biology 2024Quote: ... using the KAPA SYBR® FAST qPCR Master Mix (2×) Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.4 mM dNTPs mix, 10 x primer master mix (1.6 µM FIP / BIP, 0.2 µM F3 / B3) (Sigma, HPLC purified), 1 x EvaGreen dye (Biotium) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.4 mM dNTPs mix, 10 x primer master mix (1.6 µM FP / RP, 0.8 µM IF / IB) (Sigma, HPLC purifed), 1 x EvaGreen dye (Biotium) ...
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bioRxiv - Biophysics 2023Quote: ... Glucose (≥ 99.5% GC, Sigma Aldrich, Germany), Trolox (98% ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using the KAPA SYBR FASTqPCR kit Master Mix (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... we added 11 µl of KAPA2G Robust PCR master-mix (Sigma Catalogue No. KK5005), comprising 4 µl KAPA2G buffer ...
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bioRxiv - Microbiology 2020Quote: ... A second round of PCR performed with KOD Hot Start Master Mix (Millipore, 71842) supplemented with 1mM MgCl2 under the following parameters ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of a master mix containing 0.2% DDM (D4641-500MG, Sigma Aldrich, Germany), 100mM TEAB (17902-500ML ...
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bioRxiv - Biophysics 2024Quote: ... the cell pellet was resuspended in 250 µL of BugBuster© Master Mix (Millipore) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... ≥ 99.5% (GC) was purchased from Sigma Aldrich, France ...
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bioRxiv - Neuroscience 2023Quote: ... 2X SSC (Sigma), 0.2% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... supplement with 10% fetalclone II serum (HyClone) and 1X antibiotic mix (penicillin-streptomycin) (Sigma) and 2 mM L-glutamine (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... a master mix of desired antibodies in 25 µL FACS buffer containing 0.05% saponin (Sigma) against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ ...
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bioRxiv - Systems Biology 2020Quote: RT-qPCR was carried out using FastStart Universal SYBR Green Master Mix (Rox) (Sigma Aldrich) in an Applied Biosystems QuantStudio 6 ...
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... 2 μL of sample was added to 1x PCR Master Mix (Sigma-Aldrich, Basel, Switzerland) and the 4 degenerate primers at a final concentration of 1 μM each in a final volume of 25 μL ...
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bioRxiv - Bioengineering 2019Quote: ... the pellet was resuspended in 5 mL of the BugBuster Master Mix (Merck Millipore #71456) per gram of wet cell weight with EDTA-free protease inhibitor cocktail (Sigma #11836170001) ...
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bioRxiv - Synthetic Biology 2019Quote: We used the commercial kit: myTXTL from Arbor Biosciences (Sigma 70 Master Mix Kit, (USA). We used both our plasmid (10 nM final concentration ...
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bioRxiv - Genomics 2019Quote: ... We performed qRT-PCR using FastStart Universal SYBR Green Master Mix with ROX (Sigma, 4913914001) on a ViiA 7 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... cDNAs were mixed with indicated gene-specific primers and SYBR green PCR Master Mix (Sigma), and qRT-PCR was performed on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Microbiology 2022Quote: ... 25 μl of KOD polymerase (KOD Hot Start Master Mix, Sigma-Aldrich, Cat. No. 71842), and water for the final volume of 50 μl ...
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Physiology 2023Quote: ... AAV genome copy numbers were quantified with qPCR using FastStart Probe master mix (Sigma, Germany) on a CFX96 thermal cycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 25 µL of KOD polymerase (KOD Hot Start Master Mix, Sigma-Aldrich, Cat. No. 71842), and water for a final volume of 50 µL ...
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bioRxiv - Zoology 2019Quote: ... (±)-geosmin (Sigma-Adrich, Cat. Nr. UC18, ≥98% (GC)) ...
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bioRxiv - Neuroscience 2023Quote: ... Nonanol (1-nonanol, Sigma Aldrich, purum, ≥ 98.0% (GC) - concentration ...