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Citations for Millipore Sigma :
5051 - 5100 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... gut samples were treated with D-amino acid oxidase from porcine kidney (PkDAAO) (Catalog# A5222, Sigma-Aldrich, St. louis, MO). The sample was mixed with PkDAAO (15 U/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... 70,000 neurons were plated onto 12 mm glass coverslips coated with 10 μg/mL poly-D-lysine (PDL, Sigma P6407) and 2 μg/mL laminin ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and CasX2 expression induced with the addition of 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, EMD Millipore, Burlington, MA). After 16 hours at 16°C ...
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bioRxiv - Microbiology 2023Quote: 5 x 107 cells for each Jurkat cell line were incubated in the presence of 10 μM 5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB, Sigma) or DMSO vehicle control for 2 h under normal culture conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... Zygotes were flushed from oviducts the next morning (gd 0.5) and maintained in M2 medium (MR-015-D, EMD Millipore) supplemented with bovine serum albumin (A9647 ...
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PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Cell Biology 2023Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... and breakdown glycogen was assessed using physiological cues such as hypoglycaemia: 1,4-dideoxy-1,4-imino-d-arabinitol (DAB) (Sigma-Aldrich, UK) or drugs including ...
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bioRxiv - Neuroscience 2023Quote: ... were obtained on the hydrochloride salts as a solution in anhydrous d6-DMSO (∼20 mg/mL) (>99.9% D, Sigma-Aldrich) on a Bruker Avance III with PA BBO 400S1 BBF-H-D-05 Z plus probe (Bruker Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... 400 µL of growth media (acclimated in incubator 1-hour prior) was added to each nucleovette and cells transferred to plates pre-coated with poly-D-Lysine (Sigma). Cells were left to incubate for 24-hours ...
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bioRxiv - Microbiology 2023Quote: ... infected with 2 x 106 ME49 tachyzoites that had been pre-incubated with 1.5 μM cytochalasin D during 15 minutes at 37°C (Sigma-Aldrich); (d ...
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bioRxiv - Neuroscience 2023Quote: ... cortical neurons were dissociated from the dissected rat cerebral cortex by digestion in 0.25% trypsin-EDTA (Biological Industries) and then plated on poly-D-lysine-coated (Sigma-Aldrich) 12-mm glass coverslips in 24-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... the growth media was replaced with growth media supplemented with 10 µg/ml of Actinomycin D (Sigma Aldrich: A4262-5MG). Cells were collected at time points ...
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bioRxiv - Developmental Biology 2023Quote: ... DRG explants from E12.5 mouse embryos were dissected as described above and cultured for 18-24 hours in DRG growth medium on 8-chamber glass slides coated with 1 mg/ml poly-D-Lysine (PDL) (Sigma) and 5 μg/ml laminin (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 0.5 × 106 cells per well in six-well plates coated with poly-D-lysine (Sigma-Aldrich). Neuronal cultures were maintained at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and 1 mM TCEP) using small-volume dialysis tubes (D-tube Dialyzer Mini; Millipore Sigma; Burlington MA, USA). Protein concentration was re-measured using the same 1% mass extinction coefficient as described previously ...
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bioRxiv - Biochemistry 2023Quote: ... The two remaining holo-SmLPMO10A samples were then added to each of the NMR tubes and 10% D2O (99.9%, D, Sigma-Aldrich) was added to the samples.
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bioRxiv - Biochemistry 2023Quote: ... The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence) and 10% D2O (99.9% D, Sigma-Aldrich) was added.
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bioRxiv - Cell Biology 2023Quote: ... coli strains BL21 (D3E)/ pRARE carrying the corresponding expression vectors were induced for protein expression with 0.5 mM isopropyl-1-thio-D-galactopyranoside (IPTG, Sigma-Aldrich) for three hours at 30 ° C ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... and overexpression of the proteins was induced by 0.1–0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma Aldrich, #I5502) for 20 hrs at 18°C for His6-tagged caltubin or 3 hrs at 30°C for GST-tagged caltubin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... they were placed in the dissection media with collagenase D/ Dispase II (SIGMA cat no. 11231857001/SIGMA cat no. D4693) at 37°C for 4-5 hours in an orbital shaker ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2023Quote: Bacterial cultures were grown at 30 °C to an OD600 of around 0.8 in LB broth and induced with Isopropyl-β-D-thiogalactoside (IPTG, Sigma) for 15–18 h at 16 °C ...
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bioRxiv - Bioengineering 2023Quote: ... sequentially coated with a heavy molecular weight poly-d-lysine (50 μg/mL diluted in 0.1M Borate Buffer; Sigma P1024) and laminin (10 μg/mL diluted in DPBS ...
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bioRxiv - Immunology 2023Quote: ... mice were anaesthetized and perfused intracardially with 20ml ice-cold PBS and tissues isolated to HBSS containing 5% FBS before enzymatic digestion in Collagenase D (1mg/ml; Sigma) and DNase1 (100ng/ml ...
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bioRxiv - Immunology 2023Quote: ... Sections were developed for 5 min in DAB solution (3,3’diaminobenzidine; 5 mg/ml DAB, 0.1M PB, 0.000036% H2O2; Sigma-Aldrich D-5637). Acute neuronal loss in the CA1 region of the hippocampus was quantified as described previously2.
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bioRxiv - Neuroscience 2023Quote: ... 70,000 neurons were plated onto 12 mm glass coverslips coated with 10 μg/ml poly-D-lysine (PDL, Sigma P6407) and 2 μg/ml laminin and incubated at 37°C in 10% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein overexpression was induced by adding 0.5 mM (Cyt-b5) or 0.4 mM (FBD) isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich). Cyt-b5 was overexpressed at 37 °C for ∼18 h and FBD was overexpressed at 30 °C for ∼16 hours ...
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bioRxiv - Microbiology 2023Quote: ... media was removed and replaced with fresh media with the addition of 10 µM cytochalasin D (Sigma, St. Louis, MO) to select wells with incubation for 1 hour prior to the addition of bacteria ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the media was discarded and the cells were loaded with 75 µL 0.5 mg/mL D-Luciferin (L2916, Sigma Aldrich) in Leibowitz’s L-15 medium followed by incubating for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... d(AA)-3’ and antisense strand: 5’-r(UUU GCC AUG GCA GAA AUA GGC) d(TT)-3’ were purchased from Sigma–Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... To prevent chromatin decondensation an inhibitor cocktail of the DNA intercalator Actinomycin D (A1410, Sigma; final concentration: 10 pg/mL), topoisomerase II inhibitor ICRF-193 (I4659 ...
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bioRxiv - Bioengineering 2023Quote: ... The stressed (actively pulling) state was first acquired followed by a treatment with Cytochalasin D (dissolved in DMSO, Sigma Aldrich) at a final concentration of 4 µM for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK-Slack cells were plated at a density of 50,000 cells per well on a poly-D-lysine coated (75 μg/mL, Sigma Aldrich) 96-well ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in serum medium and seeded onto coverslips precoated with poly-D-lysine hydrobromide (Sigma; 50 µg/ml). 1 h later ...
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bioRxiv - Pathology 2023Quote: Mouse epidydimal adipose tissue was harvested and digested in Hanks’ Balanced Salt Solution (HBSS, no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml, Sigma-Aldrich) for 30 minutes at 37°C under agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were cultured as described in normoxia and hypoxia in the presence or absence of 10 μg/mL Actinomycin D (Sigma). Cells were lysed with Trizol at the specified time points for subsequent RNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 6 cm dishes one day prior to treatment with 1 μg/ml actinomycin D (Sigma-Aldrich) for the indicated time ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae that were corrected for auxotrophies at all loci were cultured in synthetic defined yeast media (SD-min) made with 2 % (w/v) D-glucose-13C6 (Sigma), 0.225 % (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... or SCLIM (Fig. 5C, D). Perfusion experiments (Fig. 5E-G) were performed using a microfluidic perfusion chamber system (CellASIC ONIX2, Millipore) with a flow pressure of 2 psi at 20°C according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was diluted in 2.5 ml of culture medium and plated in 35 mm culture dish previously coated for at least 2 hours with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich) in the incubator ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
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bioRxiv - Neuroscience 2023Quote: ... Live cells were counted using trypan blue and fifty thousand cells were plated in a 40-50 µl convex droplet onto wells of a 48-well MEA plate (Axion BioSystems) that had been pre-coated with 50 µg/mL poly-D-lysine (Sigma) in either 0.1M borate buffer or H20 ...
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bioRxiv - Neuroscience 2024Quote: ... Harrick Plasma) and coated with 500 µL per dish of 0.1 mg mL−1 Poly-D-lysine (PDL) (P6407, Sigma Aldrich) in Phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).