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Citations for Millipore Sigma :
5151 - 5200 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and C-terminal (YAPGTGGGATI-NH2, 1.5 mg) regions of proGATI were individually conjugated to keyhole limpet hemocyanin (KLH; 3 mg; Sigma H-7017, Lot 110K4833). An additional Cys residue added to the N-ter peptide allowed conjugation to KLH using m-maleimidobenzoyl-N-hydroxysuccinimide ester ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were added for 30 min to 1 h at the following dilutions: mouse anti-polyubiquitin FK1 (1:250; EMD Millipore, 04-262), guinea pig anti-p62 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed for 1 h at RT by addition of 26.6 μL 16% PFA and 4 μg/ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS using standard bore tubing cartridges ...
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bioRxiv - Biophysics 2020Quote: Goat anti-mouse IgG was incubated using a 5 x excess of N-hydroxysuccinimidyl esters – Alexa647 (LifeTech, A20106) and purified after 4 h using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML) to remove excess dyes ...
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bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... DCs were treated on day 7 of culture for 24 h with either 10−6M 1,25 dihydroxy-cholecalciferol (Sigma-Aldrich, St. Louis, MO), 10−6M 1,25 dihydroxy-cholecalciferol and 100 ng/ml LPS (Escherichia coli ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum containing 0,3% Triton in PBS for 1 h at RT followed by incubation for two overnights at 4°C with mouse anti-acetylated α-tubulin (1:500, Sigma-Aldrich, T7451) and rabbit anti-βIII-tubulin (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... 2.04 × 108 Huh-7.5-Cas9 cells were transduced by spinoculation at 1,000 g x 1 h in media containing 4 µg/ml polybrene (Millipore, cat. #TR-1003-G) and 20 mM HEPES (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... The plates were grown at 30 °C shaking at 700 RPM for 16 h covered by an AeraSeal gas-permeable sheet (EXCEL Scientific Cat. No. BS-25) after which all-trans retinal (Sigma Cat. No. R2500) was added to a final concentration of 50 μM and the plates were incubated in the dark at RT with shaking at 100 RPM for 4 h ...
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bioRxiv - Neuroscience 2020Quote: ... Adult animals (age 8-12 months) of either sex were dark-adapted for 2 h and euthanized by immersion in 1 mM tricaine methanesulfonate (SIGMA, cat. no. A5040) in pH 7.0 buffered system water prior to experiments.
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and blocked for 1 h at RT with a serum containing 3% bovine serum albumin (BSA; Sigma−Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were stained using primary antibodies for 3 h at RT followed by Duolink in situ PLA probes with anti-rabbit MINUS (Sigma-Aldrich, Cat. # DUO92005), anti-mouse PLUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... lentivirally transduced 293T cells were cultured for 48 h in serum-free DMEM supplemented with Protease Inhibitor Cocktail (1/800) (Millipore Sigma, Burlington, MA). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with Bodipy 409/505 1 ng/mL for 1 h and then incubated with DAPI (Sigma-Aldrich, 1 μg/ml) for 5 min at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.7 mL of supernatant was dialyzed against water for at least 12 h using Pur-A-Lyzer Midi 3500 dialysis mini-tubes (Sigma-Aldrich, PURD35100-1KT) to remove monomeric fucose from the sample.
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bioRxiv - Neuroscience 2020Quote: ... We incubated free-floating CeA coronal sections (16 µm) for 2 h at 30 °C with rabbit anti-FG antibody (Millipore; AB153I; 1:200) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Microbiology 2021Quote: ... Water was collected within a depth of 0.5 m in 2 L plastic bottles and filtered within 4 h in the lab through 0.22 μm Sterivex® filters (EMD Millipore, Darmstadt, Germany) connected to a peristaltic pump (EMD Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in PBS for 5 min at room temperature and incubated 2 h at room temperature with the appropriated secondary antibodies with DAPI (Sigma-Aldrich, Cat#D9542). Sections were again washed three times with PBS for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Genetics 2021Quote: ... were performed prior to incubation for 8 h with biotinylated mouse monoclonal antibody 4G8 (2 μg/ml, Sigma-Aldrich SIG-39240 Beta-Amyloid). Staining was completed with the Ventana XT DABMap kit and a haematoxylin counterstain ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured at 37 °C for 24 h, followed by various concentrations (0, 1, 2, 5, 10 µM) of lomitapide (Sigma Aldrich, Missouri, USA). The plate was incubated at 5 % CO2 at 37 °C for 24 h after treatment with lomitapide ...
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bioRxiv - Cell Biology 2021Quote: Dissected ovaries were treated for 2 h with inhibitors of aPKC (CRT-006-68-54, Bio-Techn Sales Corporation, 10 μM) or Pak1 (IPA-3, Millipore Sigma, 50 μM) as described (Chartier et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
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bioRxiv - Neuroscience 2021Quote: ... followed by further digesting the lamina propria with DMEM/F12 media containing 0.25 mg/ml collagenase H (Sigma-Aldrich, St. Louis, MO, USA) for up to 10 minutes ...
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bioRxiv - Immunology 2021Quote: The mice lungs were obtained at 24 h postinjection with DiI-stained liposomes and digested with collagenase type I (Sigma, St. Louis, MO) to obtain single cells ...
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bioRxiv - Immunology 2022Quote: ... we washed the wells and treated them for 1 h at RT with 1:30,000 diluted Extravidin-ALP conjugate (Sigma, St. Louis, MI, USA). After washing ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at room temperature and incubated overnight at 4 °C with mouse anti-FLAG monoclonal antibody (Catalogue no. F3165, Millipore Sigma, MO, USA) diluted 1:1000 in Milk/PBST ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated for 1 h at room temperature with sheep anti-mouse horseradish peroxidase (HRP)-conjugated antibody (Catalogue no. GENA931, Millipore Sigma, MO, USA) diluted 1:10000 in Milk/PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... overnight and then with primary antibodies for 48 h at 4°C: rabbit-α-rab3 (1:250; provided by S.J. Sigrist, unpublished) and chicken-α-GFP (1:150, Sigma Aldrich, AB_90890). After a further round of washing ...
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... This was followed by a second 5-minute incubation in dissecting solution supplemented with 1.6 mg/ml collagenase H (Sigma-Aldrich, St. Louis, MO), 0.5 mg/ml elastase (Worthington Biochemical ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... staining of sections was used to evaluate neuronal loss within the hilar region of the DG using Mayer’s H medium and alcoholic eosin Y (Sigma-Aldrich, Saint Louis, MO). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... in a staining jar and incubated for 2 h at room temperature in blocking solution containing 5% normal goat serum (Millipore Sigma, Item# G6767) in 1% Triton X-100 (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed again as above and 100 μl of a goat anti-rabbit IgG (H + L) alkaline phosphatase conjugate (Sigma, St. Louis, MO) diluted 1:2,000 in PBS/1% NFM were added to each well and incubated for 1.5h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Stretching is performed using 2x2 cm flexible polydimethylsiloxane (PDMS) membranes (Sylgard184; crosslinker-to-base ratios of 1:14; cured for 24 h at 65°C, Sigma-Aldrich, St. Louis) as described in (45) ...
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bioRxiv - Bioengineering 2023Quote: ... Pathological assessments were carried out using H&E and Masson’s trichrome staining in accordance with the manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2023Quote: ... leptin and insulin levels were measured in 12 h-fasted mice using a Mouse Metabolic Hormone Magnetic Bead-based immunoassay kits (Millipore Corp, Billerica, MA) according to the manufacturer instructions on samples obtained at the sacrifice.
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bioRxiv - Molecular Biology 2023Quote: ... was incubated for 2 h at 55 °C and for 10 min at 95 °C in 50 μL lysis solution (proteinase K -Sigma-Aldrich, Munich, Germany) freshly diluted 1:100 in lysis buffer (25 mM NaOH ...
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bioRxiv - Neuroscience 2023Quote: ... slow developmental (S46, 1:50; Developmental Studies Hybridoma Bank; Iowa City, IA) and rabbit anti-NF-H (1:500; EMD Millipore; Burlington, MA) for 24 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 10% FBS/PBS for 1 h and incubated with the anti-G-quadruplex antibody (BG4, Sigma-Aldrich, 1:200) overnight at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Biochemistry 2023Quote: ... Protein pellets were resuspended in 50 µL of 50 mM NH4HCO3 and digested for 16 h with trypsin (Sigma-Aldrich, Product code T6567) with an enzyme to protein ratio of 1:20 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Microbiology 2023Quote: ... Lungs from immunized mice were harvested and digested for 1 h at 37°C in digestion buffer consisting of RPMI media supplemented with Collagenase (Sigma, 2 mg/ml) and DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked with 10% FBS/PBS for 1 h and incubated with the anti-G-quadruplex antibody (BG4, Sigma-Aldrich, 1:200) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was incubated for 12 h (or overnight) in bleaching solution containing hydrogen peroxide solution (H2O2; Cat. No. H1009; Sigma-Aldrich, Merck, Germany) at a final concentration of 15% (v/v)) ...