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Citations for Millipore Sigma :
5101 - 5150 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... all types of cells were cultured in media supplemented with exosome-free FBS and treated with 50ng/mL of TNFα (R&D) and 50nM Angiotensin II (Sigma). Conditioned media was harvested every two days and combined to isolate cell-derived exosomes from the conditioned media (described below) ...
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bioRxiv - Cell Biology 2024Quote: ... 35 mm dishes were spiked with 0.05% sodium azide (Fischer #11-966-025) and 10 mM 2-deoxy-d-glucose (Sigma #D3179). For live-cell imaging of individual filopodia ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed and infectious foci were visualized by a colour reaction using 1.4 mM 3,3’-Diaminobenzidine tetrahydrochloride hydrate (Sigma, D-5637), 0.5 g/L Ammonium nickel(II)sulfate hexahydrate (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).
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bioRxiv - Physiology 2024Quote: ... and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on poly-D-lysine (Cat# P6407, Sigma)-coated 75cm2 flasks with DMEM containing 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were mechanically dissociated by repeatedly pipetting the tissue up and down in a flame-polished Pasteur pipette and then plated on poly-D-lysine (PDL, Millipore) and laminin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... approximately 30 g of spleen tissue was placed in R10 supplemented with 10U/mL DNase I and 3 mg/ml collagenase D (Sigma), the capsule was removed ...
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bioRxiv - Microbiology 2024Quote: ... globosum NCIMB 702245 were performed by culturing bacterial strains in 40 ml mMRS supplemented with: 0.5% D-glucose (Sigma-Aldrich), cooked starch substrates 1% w/v high amylose maize starch HylonVII ...
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bioRxiv - Microbiology 2024Quote: ... pseudodiphtheriticum with pJOE7706.1-tdTomato plasmid was diluted into BHI broth to an A600 of 0.002 along with 0.2mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Sigma Aldrich) to induce tdTomato expression and 100µg/mL kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... digested in HBSS with 5% FCS with collagenase D (1 mg/mL) and DNase I (0.15 mg/mL, Sigma-Aldrich) for 30 min at 37°C with constant agitation and filtered through a 70μm cell strainer ...
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bioRxiv - Microbiology 2024Quote: ... digested in HBSS with 5% FCS with collagenase D (1 mg/mL) and DNase I (0.15 mg/mL, Sigma-Aldrich) for 30 min at 37°C with constant agitation and filtered through a 70μm cell strainer ...
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bioRxiv - Microbiology 2024Quote: ... for 5 min and plated them with astrocytes on plates coated with 9 to 16 μg/cm2 poly-d-lysine and ∼1.5 μg/cm2 laminin (from a mouse Engelbreth-Holm-Swarm sarcoma; Sigma Aldrich). The densities we used are listed below ...
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bioRxiv - Immunology 2024Quote: ... 400 µL of growth media (acclimated in incubator 1-hour prior) was added to each Nucleocuvette and cells transferred to plates pre-coated with poly-D-Lysine (Sigma). Cells were left to incubate for 24-hours ...
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bioRxiv - Molecular Biology 2024Quote: ... blocked with 1% BSA and incubated with blocking buffer containing a 10% protein lysate in the presence or absence of D-mannose (2.5%, M6020, Sigma Aldrich) for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: Glucose metabolism was inhibited with the glucose analog 2-Deoxy- D-Glucose (2-DG; Sigma Aldrich, Catalog No. D6134-25G) which was stored at -20°C at a stock concentration of 2 M (25 g crystalline powder in 76.14 mL dH2O) ...
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bioRxiv - Immunology 2024Quote: ... Collagen gels were dissociated using pipette tips and digested at 37°C for 45 minutes using 2 mg/mL Collagenase D (Sigma) in combination with gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... hybrid PLGA-lipid NPs were synthetized via self-assembly of poly(D,L-lactide-co-glycolide) acid (30-60 kDa, lactide:glycolide 50:50; Sigma #P2191) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] (sodium salt ...
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bioRxiv - Cell Biology 2024Quote: ... To assess glycolysis-related ECAR an additional injection of 100 mM 2-deoxy-d-glucose (2-DG, from Sigma-Aldrich), an inhibitor of glycolysis ...
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bioRxiv - Neuroscience 2024Quote: ... Harrick Plasma) and coated with 500 µL per dish of 0.1 mg mL−1 Poly-D-lysine (PDL) (P6407, Sigma Aldrich) in Phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... approximately 60-80 mm3 of tumor tissue was finely minced with a sterile scalpel and dissociated for 1 hour at 37°C with shaking in 9 mL of an enzyme solution containing 1 mg/mL collagenase D (Sigma) and 100 ng/mL DNAse I (≥40 U/mL final ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 10,000 cells were seeded into 12-well plate pre-coated with 100 μg/ml poly-D-lysine (Sigma Aldrich) and 10 μg/ml laminin (Thermo Fisher) ...
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bioRxiv - Bioengineering 2024Quote: FMC63 CAR T cells were treated with small-molecule inhibitors targeting Actin (inhibitor: Cytochalasin D, Sigma Aldrich catalog no. C8273), Dynamin (inhibitor ...
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bioRxiv - Biophysics 2024Quote: ... cells were seeded at a density of 0.4×105 cells/ml on poly-D-lysine-coated coverslips and supplemented with 1 µM all-trans-retinal (Sigma-Aldrich). After one day ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 160µl E.coli solution containing either 5µM Cytochalasin D (Sigma- Aldrich, Cat#: C8273) as a negative control for phagocytosis ...
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bioRxiv - Neuroscience 2024Quote: ... EZVIEW Glass Bottom Culture Plates LB (IWAKI) were coated with 10 µg/mL poly-D-lysine hydrobromide (Sigma Aldrich Technologies) for one day (4°C) ...
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bioRxiv - Neuroscience 2024Quote: ... Vermis slices were incubated with an antibody to Calbindin (Calb; monoclonal anti-Calbindin-D-28K; Sigma catalog # C9848; 1:500), a highly selective marker for PCs (Garcia-Segura et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and pHrodo-labeled myelin (48 µg/ml) either without or in combination with Cytochalasin D (40 µM, #C2618, Sigma-Aldrich), which inhibits phagocytosis ...
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bioRxiv - Neuroscience 2024Quote: ... Cells from 2 brains (n = 4 cortices) were plated in a T-75 flask coated with poly-d-lysine (Sigma) in 15 mL of DMEM/F12 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Rat cortical neurons were dissociated from the dissected rat cerebral cortex by digestion in 0.25% trypsin-EDTA (Biological Industries) and plated on poly-D-lysine–coated (Sigma-Aldrich) 12-mm glass coverslips in 24-well plates ...
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bioRxiv - Microbiology 2024Quote: ... and their lungs were minced with scissors and digested in 3 ml of RPMI-1640 with 5% heat-inactivated FBS containing 1 mg/ml collagenase D (Sigma) and 30 µg/ml DNAseI (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... or on coverslips (for immunocytochemistry assay) previously coated with poly-D-lysine (0.1 mg/mL) with neuronal plating medium (MEM supplemented (Sigma-Aldrich), 0.6% glucose and 1 mM pyruvic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with UPEC UTI89 WT or ΔfimH (OD600 0.02) for 30 min on a shaking platform in the presence or absence of D-mannose (2.5%, M6020, Sigma Aldrich). The slides were then washed with PBS ...
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bioRxiv - Biophysics 2024Quote: ... and imaged in 0.4 ml/well Oxyrase-based STORM buffer (Phosphate Buffer Saline, 50mM Mercaptamine, 10 mM D-Lactate and 10 µl of a debris free SAE0010-5ml EC-Oxyrase Sigma).
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bioRxiv - Physiology 2024Quote: ... assessed by the presence of the second polar body and cultured in KSOM medium (Merck Millipore ref. MR-106-D). Oocytes were discarded.
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bioRxiv - Genomics 2024Quote: ... cauda epididymis and vasa deferentia from males were dissected and sperm was gently squeezed/dissected out into preequilibrated capacitation media (HTF supplemented with MBCD at final concentration of 0.75 mM) under mineral oil (HTF: Millipore MR-070-D; MBCD: Sigma-Aldrich C4555 ...
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bioRxiv - Genetics 2024Quote: ... mNPCs were cultured in media conditions listed above on plates coated firstly with poly-D-Lysine (PDL) hydrobromide (Sigma, P7280) overnight followed by laminin (Roche ...
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bioRxiv - Cancer Biology 2024Quote: Sterile coverslips were placed in 12 multiple wells and the surface was covered with poly-D-lysine and laminin (Sigma). The plate was incubated at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... thawed GST-BirA enzyme (20 µL of 50 µM solution) and D-Biotin (3 µL of 50 mM solution in 100 % DMSO; Sigma) and incubated for 1 h minimum at 30 °C in an incubator with gentle shaking (90 rpm) ...
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bioRxiv - Biochemistry 2024Quote: First culture plates were coated with 200µL of poly-D-lysine (0.1mg/mL in dH2O, Merck-Sigma, Cat. No. P6407) and incubated overnight at 37°C in a 5% CO2 environment ...
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bioRxiv - Biochemistry 2024Quote: ... Cultures were plated at a density of 2.1 x 106 cells/mL on glass coverslips precoated with poly-D-lysine (100 µg/ml, Sigma-Aldrich) and laminin (50 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed in lysis buffer (30 mM Tris pH 7.5, 120 mM NaCl, 20 mM NaF, 0.5% octyl-ϕ3-D-glucopyranosid) (Sigma-Aldrich, #O8001) and protease inhibitors (10 µg/mL leupeptin ...
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bioRxiv - Cell Biology 2024Quote: ... followed by the addition of 6µL of 60mM triethylammonium bicarbonate (TEAB, Supelco 18597) (pH 8.5) supplemented with 0.013% n-Dodecyl-beta-D-maltoside (DDM, Sigma-Aldrich D5172). Plates were sealed and incubated at 95°C for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... 100,000 neurons were plated onto 12 mm glass coverslips coated with 10 μg/mL poly-D-lysine (PDL, Sigma P6407) and 2 μg/mL laminin and incubated at 37°C in 10% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, D-404S) was obtained from AccuStandard and solubilized in dimethyl sulfoxide (DMSO, D8418, Sigma-Aldrich). 17β estradiol was purchased from Sigma-Aldrich (3301 ...
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bioRxiv - Biophysics 2021Quote: ... the sample was washed three times with PBS-/- again and blocked for 1 h at room temperature using PBS-/- supplemented with 20 % goat serum (GS, Sigma, St. Louis, USA) and 0.2 %Triton-X-100 (Carl Roth ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated for 1 h in 1 % Sirius Red solution and washed twice with 0.5 % acetic acid (Sigma-Aldrich, St. Louis, USA). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... The springs and the jar lids were cleaned (60°C, 1 h) in a 100 g/L sodium persulfate solution (Na2S2O8, Sigma-Aldrich, Buchs, Switzerland), then rinsed with nanopure water ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked for 1 h in Tris-buffered saline (TBS) with 5% milk and 0.05% Tween 20 (Sigma-Aldrich, St. Louis, MO) and washed 3 times (5 min each ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2019) or the background strain CEA17) were incubated 4 h at 37°C in Czapek-Dox medium (CZD, Sigma-Aldrich Chemie, Munich, Germany) to induce swelling ...