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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... we incubated them with anti-rabbit PLUS and anti-mouse MINUS PLA probes (1 hour at 37°C) diluted in Duolink® incubation buffer (1:200; Sigma), followed by three washes with PBS ...
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bioRxiv - Physiology 2020Quote: ... We isolated primary rat neonatal cardiomyocytes (RNCs) and cultured them at 37°C with 1% CO2.2 RNCs were seeded on laminin-coated (Sigma, MO, USA) µ-Slide 8 Well Grid-500 ibiTreat dishes (ibidi) ...
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bioRxiv - Physiology 2020Quote: ... On day 4 cells were re-stimulated with the same concentrations of anti-CD3/CD28 for 6 hours at 37°C in the presence of 1 μg/ml brefeldin-A (Sigma-Aldrich). As a control ...
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bioRxiv - Pathology 2021Quote: ... cryosections were transferred to TBS and incubated for 1 h at 37 °C with anti-mouse PLUS and anti-Rabbit MINUS PLA probes (Sigma-Aldrich). Then ...
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bioRxiv - Neuroscience 2021Quote: ... 6 ml 85% ortho-phosphoric acid preheated to 120 °C in an oil bath were added to 400 mg of (±)-epinephrine hydrochloride (E4642, Sigma-Aldrich) in a 2-dram glass scintillation vial ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were centrifuged for 10 min at 5000Xg and supernatants were diluted 1:1 with PBS (vol/vol) containing protease/phosphatase inhibitors and incubated overnight at +4°C with streptavidin-agarose beads (EMD Millipore). Beads were washed twice with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... RPMI 1640 complemented with indicated inhibitors was replaced daily for 3 days before overnight fixation with formalin at 4°C (Sigma-Aldrich).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... brain slices were first incubated in a 2N-HCl for 45 minutes at 45°C and then rinsed in a boric acid (Sigma-Aldrich) solution 0.1 M at pH= 8.5 for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... were cleaned by incubation at 56°C overnight in 2% Hellmanex III (Hellma Analytics) dissolved in ultrapure Milli-Q® water (Millipore) followed by 30 minutes sonication in a heated sonication bath ...
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bioRxiv - Biochemistry 2021Quote: ... Luminol (cat #A-8511) and p-coumaric acid (cat # C-9008) used to make chemiluminescence solution for western blotting were purchased from Sigma-Aldrich.
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bioRxiv - Biochemistry 2021Quote: ... 1:2000 dilution), rabbit anti-H3K79 (SAB5600231-100UG, 1:2000 dilution), rabbit anti-c-MycK323ac (ABE26, 1:2000 dilution) were from Millipore Sigma. Rabbit anti-p65K310ac (ab19870 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue was then washed in PBS containing 0.03% tween-20 for two minutes following overnight incubation at 4°C with anti-versican GAGβ (1:100 dilution, rabbit source, Millipore AB1033). Tissue was then washed three times in PBS containing 0.03% tween-20 for fifteen minutes followed by secondary antibody staining for one hour at room temperature (anti-rabbit Alexafluor 488 ...
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were cultured in a humidified 5% CO2 incubator at 37 °C and routinely tested for mycoplasma (Sigma-Aldrich). All media were supplemented with 100 U ml−1 penicillin and 100 µg ml−1 streptomycin (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were digested for 4 h on a filter with 1:50 Lys-C (Wako) and thereafter overnight with 1:50 proteomics-grade dimethylated trypsin (Sigma-Aldrich) as described for the filter-aided sample preparation protocol 42 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed and washed with FoxP3-staining-buffer-set (eBioscience) and then incubated with DNase at 37°C for 30 mins (1mg/ml in D-PBS, Sigma Aldrich), DNase-solution 30ml DNase-stock + 960ml D-PBS + 10ml MgCl2) ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin conjugates were eluted at 65 °C for 15 min in 2x Laemmli buffer containing 200 mM imidazole and 5% 2-mercaptoetanol (Sigma, M6250), vortexed for 90 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were then treated for one hour at 45 °C with 1 μL of proteinase K (10 mg/ml stock; Millipore Sigma) and 1 μL of RNase A (24 mg/mL stock ...
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bioRxiv - Cancer Biology 2020Quote: PKO cells transfected with Flag-tagged OGT before cultured in 5 mM glucose were collected and incubated overnight at 4°C with ANTI-FLAG M2 affinity gel (Sigma, A2220). Immunoprecipitated complexes were eluted and stained with Coomassie blue ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained at 37°C and 5% CO2 and the growing media was supplemented with 1% penicillin/streptomycin (Sigma, UK) and 1% L-glutamine (Sigma ...
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bioRxiv - Immunology 2020Quote: ... The DNA solution was heated at 50°C for 10 minutes and mixed with an equal volume of 2% agarose (Sigma-Aldrich). The mixture was poured onto a plastic casting tray and left at room temperature till it solidified ...
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bioRxiv - Cancer Biology 2021Quote: ... DMSO by first incubating the samples in freezing solution at room temperature for 30 min and then transferring to -80°C in a CoolCell freezing container (Sigma-Aldrich), (2 ...
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bioRxiv - Developmental Biology 2021Quote: ... stage embryos were isolated from oviducts in M2 media (pre-warmed at 37°C for at least 2-3 hours) and thereafter cultured in KSOM (Sigma-Aldrich, EmbryoMax KSOM Mouse Embryo Media ...
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bioRxiv - Biophysics 2020Quote: After detachment the cells are spun down to the right concentration in PBS and incubated for one hour at 37°C with 40nU per cell (1x treatment) or 200nU per cell (5x treatment) of heparinase III from Flavobacterium heparinum (Sigma Aldrich). The treated cells are then immediately used for a < 2h BFP experiment.
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bioRxiv - Biochemistry 2021Quote: ... Reactions were equilibrated at 37°C for 5 min and subsequently initiated by addition of NADPH (Sigma, final concentration 2 mM). The reaction was allowed to proceed at 37°C and timepoints at 0 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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bioRxiv - Bioengineering 2021Quote: ... cell supernatant was harvested by centrifugation (4000xg for 20 mins at 4°C) and filtered through a low binding 0.22 μm stericup vacuum filter (Millipore Sigma, S2GPU10RE). The filtered supernatant was incubated for 2 hours at room temperature with 6ml of Ni-NTA resin (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... Number 78/07).78 Cells were cultured at 37 °C in a humidified atmosphere at 5% CO2 in minimal essential medium with alpha modification and nucleosides (MEMα; Sigma-Aldrich) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Elution fractions were buffer exchanged into sterile water chilled to 4 °C using 15 mL 30 kDA molecular weight cutoff devices (Millipore Sigma). Buffer-exchanged proteins were flash frozen in 50% v/v glycerol and stored at −80 °C.
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bioRxiv - Cell Biology 2021Quote: ... the TEV cleavage step was done overnight at 4°C and the cleaved proteins were concentrated using 100K MWCO concentrator (EMD Millipore). Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore ...
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bioRxiv - Biophysics 2021Quote: ... Frozen aliquots of cells (grown to saturation and stored in 25% glycerol at -80°C) were grown in Terrific Broth (Sigma-Aldrich) at 33°C for 5 h ...
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bioRxiv - Evolutionary Biology 2021Quote: An overnight culture of Bacillus thuringiensis Berliner (ATCC 55177) was prepared from a glycerol stock and grown at 30°C in Nutrient Broth #3 (NB, Sigma Aldrich). An aliquot of overnight culture was brought to an OD of 0.5 in fresh NB ...
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bioRxiv - Microbiology 2021Quote: ... and intermediated lobes were carefully removed and the bronchi were filled with 37°C low-melting agarose (GERBU, Heidelberg, Germany) in RPMI-1640 medium (Sigma-Aldrich). After solidifying on ice ...
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bioRxiv - Microbiology 2020Quote: JW18 Drosophila melanogaster cells with and without Wolbachia (strain wMel) were grown at 24 °C in Shields and Sang M3 insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... grids were incubated for 1 hour 37 °C with a 1:2000 dilution of a 10 nm gold-labelled goat anti-rabbit IgG (G7277; Sigma Aldrich), washed with 1x PBS ...
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bioRxiv - Microbiology 2021Quote: Vero cells (ATCC CCL-81) were cultured under standard conditions (37°C and 5% CO2) in Dulbecco-modified MEM (Sigma Aldrich) supplemented with 10% heat-inactivated foetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... or (2) primary anti-dsRed (Sicgen, AB8181-200, 1:200) together with primary rabbit anti-proSP-C (Sigma, AB3786, 1:1000) or primary rabbit anti-NaPi-IIb (provided by C ...
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bioRxiv - Immunology 2021Quote: ... frozen patient tumor fragments were slowly thawed at 37°C and extensively washed in PBS and warm RPMI medium containing L-glutamine (R8758, Sigma-Aldrich), supplemented with penicillin/streptomycin (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and rotated at 37 C for 40 minutes in Collagenase IV Buffer + 2 mg/mL Dispase II (Sigma Aldrich cat. 04942078001). Homogenate was filtered through a cell strainer (Corning cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was pipetted to a new 1.7 mL centrifuge tube and the acetonitrile was evaporated with warm nitrogen gas in an AutoVap (Zymark) at 70° C and then reconstituted in enough HPLC grade water (Sigma-Aldrich) for a 1:5 dilution and vortexed for 20 s.
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bioRxiv - Molecular Biology 2021Quote: ... The prepared sections on glass slides were deparaffinized for 30 minutes at 65°C and then for 2x 30 minutes in Neo-Clear (Merck Millipore, 109843). Samples were rehydrated for 2x 10 minutes in 100% ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... These parasites were cultured (10 sex-separated adults or five adult pairs per well in a 48 well tissue culture plate) at 37°C in DMEM (Sigma-Aldrich) supplemented with 10% foetal calf serum ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-c-Myc (Cat# ITG0001) was purchased from Immunotag (USA) and anti-G6PDH (Cat# A9521) was purchased from Sigma-Aldrich (USA).
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bioRxiv - Molecular Biology 2020Quote: ... The fraction was incubated for 4 hours at 4°C with 250 μl of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... The cells were washed with cold PBS and incubated with 200 μl of DNase-free RNAse A in PBS (100 μ g/ml) for 30 min at 37°C before DNA staining with 200 μl of Propidium Iodide (PI, Sigma) (50 μ g/ml ...
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bioRxiv - Immunology 2020Quote: ... The remaining lysate was incubated overnight at 4 °C on a rotating wheel using anti-V5 Affinity Gel beads (Sigma, A7345). Beads were washed three times with protein lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 transformed with recombinant pGEX-2T plasmids were diluted 1:10 with fresh medium and grown for 1 h at 37°C before inducing with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma). After another 4 h incubation at 37°C ...
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bioRxiv - Immunology 2021Quote: ... major NIH S (MHOM/SN/74/Seidman, clone A2 promastigotes were cultured at 26°C in Leishmania medium (M199 medium (Sigma Aldrich) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin 0.5 μm sections were cut and collected onto clean glass slides and dried at 60°C before staining with 1% Toluidine Blue and 1% Borax (all from Sigma-Aldrich) in distilled water for 30 seconds ...
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bioRxiv - Immunology 2020Quote: ... High Five™ Cells (BTI-TN-5B1-4) (Trichoplusia ni) were maintained at 28°C in EX-CELL 405 medium (Sigma) supplemented with penicillin and streptomycin.
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bioRxiv - Cell Biology 2019Quote: ... minced and placed in Miltenyi GentleMACS C-tubes containing 2ml dissociation buffer (RPMI media with 0.2mg/ml Liberase (Sigma Aldrich 5466202001) and 0.5mg/ml DNAase (Sigma Aldrich 11284932) ...