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Citations for Millipore Sigma :
4951 - 5000 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of monoclonal antibodies in duplicate were prepared in a 96-well microtiter plate and pre-incubated with pseudovirus for 1 h at 37 °C in the presence of a final concentration of 5 mg/mL polybrene (EMD Millipore), before the pseudovirus-mAb mixtures were added to 293T-hACE2 monolayers ...
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... High Five™ Cells (BTI-TN-5B1-4) (Trichoplusia ni) were maintained at 28°C in EX-CELL 405 medium (Sigma) supplemented with penicillin and streptomycin.
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bioRxiv - Immunology 2023Quote: ... Each PBMC sample was thawed at 37°C and washed in pre-warmed RPMI-1640 media (Sigma-Aldrich Life Sciences, USA) supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: Yp was cultured to stationary phase at 28°C and 250 rpm shaking for 16 hours in 2×YT broth supplemented with 2 μg/ml triclosan (Millipore Sigma). Mice were fasted for 16 hours and subsequently inoculated by oral gavage with 200 μl phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... 0.1% Tween 20) and incubated for 1 h at room temperature or overnight at 4°C with primary solution (anti-FLAG (Sigma F1804), diluted 1:1000 in 5% milk in TBST) ...
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bioRxiv - Cell Biology 2023Quote: ... were dissected and immunostained as described previously.77 Dissected and fixed tissue was incubated overnight at 4°C with anti-Flag (mouse, 1:200; Sigma F1804) or anti-HA (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... Single-cell suspensions from MM biopsies were generated by treating tissues for 70 min at 37°C with 250 μg/ml Liberase (Millipore-Sigma) and 100 μg/ml dispase II (Millipore-Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... The insulin-secreting cell line INS-1 (a gift from Professor C. B. Wollheim) was cultured in RPMI-1640 (Sigma Aldrich), supplemented with 10% fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 832/13 cells were cultured at 37°C and 5% CO2 in complete RPMI medium (RPMI-1640 (Sigma Cat. No. R0883) supplemented with 10% FBS (Cat ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured at 37°C in 5% CO2/95% air in Dulbecco’s modified Eagle’s medium (Sigma, St Louis, MO, USA) containing 10% heat-inactivated fetal bovine serum (Sigma) ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... 4 °C) and supernatants were transferred into new tubes containing 1μl 800 ng/μl myristic acid-D27 (Sigma; dissolved in pyridine). Next ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the recombinant proteins was induced by 1 mM isopropyl β-D-thiogalactopyranoside at 37 °;C for 5 h in host Escherichia coli strain Rosetta (DE3) pLysS cells (Novagen). After induction ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration was performed by ultrafiltration (4 °C, 3200 × g, 100-120 min) using an Amicon Ultra (MW 3,000, Millipore, Cork, Ireland). The medium change to FBS-free Opti-MEM I was required to concentrate the culture medium by ultrafiltration because the viscosity of FBS hampered the ultrafiltration.
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bioRxiv - Neuroscience 2023Quote: ... young adult animals grown at 15°C were picked without food onto a 5% agarose pad on a glass coverslip and immobilized with 20mM tetramisole (Sigma Aldrich). A second glass coverslip was placed on top and the sandwich was then placed on a Peltier device on the microscope stage pre-warmed to 25°C ...
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bioRxiv - Biophysics 2023Quote: ... in the presence of horseradish peroxidase (HRP) (stored at 1000 U/ml at -20 °C, thawed on the day of use and kept on ice; Sigma Aldrich) at a final concentration of 2 U/mL ...
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bioRxiv - Immunology 2023Quote: ... and the resulting protein was mixed with 5 molar equivalents of pSer-maleimide linkers at a concentration of 1 mg/ml for 16 hours at 4°C in tris-buffered saline (TBS, Sigma Aldrich) at pH 7.2-7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 28°C in SDM-79 media supplemented with 10% v/v heat-inactivated fetal calf serum (Sigma-Aldrich) (Brun and Schönenberger ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... one with no AmB and others containing different concentrations between 50 µg/ml and 250 µg/ml added to medium cooled to below 50 °C from a 250 mg/ml stock in water (Sigma-Aldrich) aliquoted to reduce freeze-thaw cycles and stored at -20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 50µg of each protein sample was run on a 10% SDS-PAGE gel followed with a 90V transfer at 4°C for 90 minutes to a PVDF membrane (Millipore #IPFL00010). Membranes were blocked for one hour with Intercept PBS blocking buffer (LiCor #927-40000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Molecular Biology 2023Quote: ... After the overnight growth at 37 °C, the cells were harvested by centrifugation (30 min, 4300 g) and plasmid purified by midi-prep (Millipore Sigma).
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS/ Tween20 (buffer B) and then blocked with 1% bovine serum albumin in PBS/Tween20 (buffer C) (Sigma-Aldrich). 50 µL of mouse monoclonal V5B2 or rabbit polyclonal sPrPY226 ...
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bioRxiv - Developmental Biology 2023Quote: ... – under standard culture conditions (37 °C and 5% CO2) and passaged after washing with Dulbecco’s phosphate buffered saline (PBS; Sigma-Aldrich, #D8537) using 0.025% trypsin (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Bioengineering 2023Quote: ... pH 14), hydrogen chloride solution (c(HCl)=1, pH 1), and buffer solution (pH 4, pH 9) were purchased from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Systems Biology 2023Quote: ... 50 μM and 100 μM compound for exactly 5 minutes at 25 °C in a thermocycler (Biometra TRIO) before proteinase K (Proteinase K, Tritirachium album, 10 mg, Sigma Aldrich) digestion ...
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bioRxiv - Neuroscience 2023Quote: ... were treated to prevent proliferation with cytosine β-D-arabinofuranoside (ara-c; C1768, 2.5 µM, Sigma-Aldrich, St. Louis, MO, USA) and frozen in liquid nitrogen until plated in co-culture with neurons ...
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bioRxiv - Molecular Biology 2023Quote: ... After overnight growth at 37 °C, each of the 50 mL cultures were centrifuged (30 min, 4300 g) and plasmid purified by midi prep (Millipore Sigma). The bottlenecked library displayed an estimated 16.9 fold barcode/variant coverage.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were diluted in 1X Laemmli sample buffer and heated at 95°C for 10 min in the presence of β-mercaptoethanol (Sigma) (except for OXPHOS cocktail antibody according to manufacturer’s instructions) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were seeded on coated plates (Poly-L-ornithine [0.001%; A-004-C; Sigma-Aldrich] and laminin [300ng/mL; L2020; Sigma-Aldrich]) in 2i+LIF culture medium (N2B27 Media ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-monoclonal PSD95 (1:3000, MAB1598) and synaptophysin (1:10000, MAB329-C) (Burlington, MA), mouse monoclonal anti-actin (1:10000, A5441) from Millipore Sigma. Primary antibody incubations were conducted overnight at 4 °C followed by incubation with the appropriate HRP conjugated secondary antibody for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cells are incubated with tDNA at 30 °C during 10 or 20 min followed by addition of 20 µg mL-1 DNase I (Sigma Aldrich) with MgCl2 at 10 mM for a further 10 minutes at 37 °C before plating ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 300RCF for 5mins at 4°C and supernatant was decanted before resuspension in 100μg/mL DNAse I (10104159001, Sigma-Aldrich, Canada), as per manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Genetics 2023Quote: ... The embryonic cell line (ISE6) was cultured at 34°C in L15C300 medium supplemented with 10% heat inactivated fetal bovine serum (FBS, Millipore Sigma), 0.1% bovine lipoprotein concentrate (LPPC ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli was grown at 37°C in LB medium with 100 μg/ml ampicillin and/or 20 μg/ml chloramphenicol (Sigma-Aldrich), as needed for plasmid maintenance ...
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bioRxiv - Immunology 2023Quote: ... The lungs were minced with a gentleMACS dissociator (Miltenyi) and digested (30 min, 37°C) in 250 U/ml collagenase and 60 U/ml DNase (both from Sigma-Aldrich). Lung cell suspensions were passed through a 70-μm and 40-μm strainers sequentially to remove cell clumps ...
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bioRxiv - Cell Biology 2023Quote: ... The transformed bacteria were grown overnight at 37°C in 25 ml Lysogeny broth (LB) media supplemented with Kanamycin (Sigma K0254). For autoinduction of MDH2 and eGFP proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were rapidly harvested and either snap frozen in liquid nitrogen and stored at −80°C for later biochemical analyses or processed by overnight immersion in freshly prepared fixative: 3.7% Paraformaldehyde (Sigma-Aldrich, 441244) 200 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... from each sample was reduced with 5 mM dithiothreitol (DTT) for 1 hr at 37°C and alkylated with 10 mM iodoacetamide (Sigma #I1149) for 45 min in the dark at RT ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was incubated for 1h at 4 °C in the presence or absence of 20 or 100 µM FTY720 (Sigma-Aldrich) or fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... was stored at −30 °C and used at 1 g/ L in 10 mM of calcium chloride (CaCl2) solution (Sigma-Aldrich). For the crosslinker ...