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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The C9 and VVM84 skin fibroblasts were maintained at 37°C and 5% CO2 in Minimal Essential Media Eagle (MEM) (Sigma, M4655) supplemented with 15% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Insoluble debris was removed by centrifugation at 15,000 rcf for 30 min at 4°C following which 2.1 ml of neutralized 10% polyethyleneimine (Millipore Sigma, cat#P3143) was added to the decanted supernatant dropwise while stirring at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were incubated at 37°C for 5 min before addition of 450 µl phenol/chloroform/isoamylalcohol (25:25:1, Sigma-Aldrich) and mixing by rotation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Appendixes (cut into 1-2mm pieces) were incubated at 37°C for 30 minutes in medium with 1 mg/mL collagenase IV (Sigma-Aldrich) and 1 mg/mL DNAse I (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated overnight at 4°C with an anti-GFP (ROCHE Anti-GFP, from mouse IgG1K, Millipore Sigma) (1:1000) ...
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bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was removed and the cell pellet was resuspended in 500 μL Diluent C containing 2 μL PKH67 dye (PKH67 Green Fluorescent Cell Linker Kit, Sigma-Aldrich), incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and frozen (−20°C) as stock solutions at 100 mg mL-1 for ampicillin and 30 mg mL-1 for kanamycin (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Peptides were reduced with 4 mM DTT for 30 min at 25°C and alkylated by 10 mM iodoacetamide (Sigma-Aldrich) for 45 min in dark in a thermoshaker ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then centrifuged at 2,500 × g for 10 min at 4°C and proteins from the supernatant were precipitated using 10% tricholoroacetic acid (TCA) with acetone (Sigma-Aldrich). 1,700 μl of TCA-acetone was added to 300 μl of culture supernatant and incubated at −20°C for one hour ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Neuroscience 2021Quote: Cortical synaptosomes were incubated for 90 min at 4 °C with gentle shaking on Nunc Lab-Tek 8-well chamber slides (Sigma-Aldrich) coated with poly-D-lysine (ThermoScientific) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6, G5555, Sigma-Aldrich; 1:1000 Rabbit anti-Cux1 ...
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bioRxiv - Immunology 2020Quote: ... Protein pellets were obtained by centrifugation at 12,000 rpm for 15 minutes at 4 °C and subjected to trypsin digestion with sequencing grade trypsin (1:20) (Sigma Aldrich) overnight at 37 °C.
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bioRxiv - Immunology 2020Quote: ... PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). NK cells were purified by magnetic bead isolation via negative selection according to the manufacturer’s specifications (Miltenyi ...
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bioRxiv - Cancer Biology 2020Quote: ... and the pellet resuspended and incubated for 3-7 minutes at 37°C in accutase dissociation reagent (Sigma, Poole, UK, A6964). After enzyme neutralization with fresh medium ...
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bioRxiv - Cancer Biology 2021Quote: ... Polar metabolites were derivatized for 90 min at 37 °C with 20 μl of 20 mg per ml methoxyamine (Merck Sigma, 226904) in pyridine (Merck Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Biochemistry 2021Quote: ... in a pre-cooled MLA-80 rotor at 2°C for 30 minutes and then filtered through a 0.45-μm Milliex-HV polyvinylidene fluoride membrane (Millipore, Bedford, MA). The filtrate was loaded onto an equilibrated TOG-column ...
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bioRxiv - Systems Biology 2020Quote: ... Urea concentration was subsequently reduced to 2M and proteins were digested overnight at 37°C after the addition of trypsin (Sigma-Aldrich) at a 1:100 ratio ...
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bioRxiv - Cancer Biology 2020Quote: ... for 5 min at 37°C in bicarbonate-free and serum-free growth medium containing 1 mg/mL BSA and 20 mM Hepes (Sigma-Aldrich), pH 7.3 ...
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bioRxiv - Molecular Biology 2022Quote: ... The caput of the epididymis was minced into small pieces with scissors and placed in warm (37 C) M2 culture media (M7167, Millipore Sigma) with HEPES and 2% exosome depleted fetal bovine serum (A2720803 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Physiology 2022Quote: ... and incubated on shaker at 35°C for 30 min in the same solution with the addition of elastase type IV (0.6 mg/ml; Sigma, Chemical Co.), collagenase type 2 (0.8 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Three to five weeks after reprogramming, single colonies were picked and expanded on SNL76/7 feeder cells (ATCC, SCRC-1049) treated with mitomycin C (Sigma Aldrich) in 20% KSR medium [92] ...
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bioRxiv - Developmental Biology 2022Quote: ... brain slices were transferred to 3.5 cm dishes containing 37°C warm CO2-independent microinjection medium (CIMM: DMEM-F12 (Sigma, Germany, D2906), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for two minutes and gently suspended by addition of serum-free DMEM with trypsin inhibitor (T9003, Sigma-Aldrich) (1mg of trypsin inhibitor per milliliter Trypsin EDTA Solution B) ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Donor strains were grown in 96-well deep-well plates (1-mL cultures in TY medium) for at least 5 h with shaking at 37 °C and a Breathe-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2022Quote: ... and incubating at 60 °C for 45 min followed by alkylation with 10 mM iodoacetamide (IAA, Sigma-Aldrich Cat. Nr. I1149) at RT for 30 min in darkness ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 mM 2-deoxy-D-glucose (Port C) were sequentially injected in 25 μL volume (all reagents from Sigma-Aldrich). For measuring the metabolic activity after M2d or MS-5 co-culture ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μM antimycin A together with 2 μM Rotenone (Port C) were sequentially injected in 25 μL volume to measure basal and maximal OCR levels (all reagents from Sigma-Aldrich). For ECAR measurements ...
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bioRxiv - Cancer Biology 2022Quote: ... Livers were weighted and 50% of the tissue was sliced in small pieces and incubated 30 minutes at 37°C in 10 ml of digestion medium (RPMI GlutaMAX medium [Gibco] containing 200U/ml of collagenase type IV [Sigma-Aldrich] and 100 U/ml of DNAse I [Sigma-Aldrich]) ...
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bioRxiv - Cell Biology 2022Quote: ... All plates except Total were then washed twice with ice-cold PBS and incubated for 40 minutes at 4°C with MESNA-containing buffer (Sigma Aldrich) to remove remaining surface-associated biotin ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 16 hours at 18°C in OCM with or without the addition of different mitochondrial inhibitors: 5 μM Rotenone (Sigma, R8875), 50 mM malonic acid (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Conjugates between YTS cells and KT86 at a 2:1 ratio were established in suspension for 15 min at 37°C and adhered to poly-lysine-coated glass slides (Polyprep; Sigma-Aldrich) for additional 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). NK cells and B cells were purified from thawed PBMCs by magnetic bead isolation via negative selection according to the manufacturer’s specifications (Miltenyi ...
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bioRxiv - Cell Biology 2022Quote: ... The injected embryos were incubated at ∼25°C up to the 4-cell stage and embedded into 0.5% agarose type VII-A low melting temperature (SIGMA, A-0701) in PBS on a glass bottom dish (IWAKI ...
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bioRxiv - Immunology 2019Quote: ... The sections were stored in PBS overnight at 4°C and then incubated with normal goat serum (dilution 1:50; Sigma-Aldrich) in 5 % bovine serum albumin /0.05 M tris-buffered saline to avoid non-specific binding for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... All 78 spiked and control soil microcosms were enriched for 24 h at 30 °C in 45 ml of LB supplemented with ampicillin (10 mg/l, Sigma Aldrich). Five hundred µl aliquots of enrichment broth were sampled before and after the 24-h enrichment step ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were maintained on a layer of 40k per cm2 of mouse embryonic fibroblasts mitotically inactivated by Mitomycin C (Sigma M4287). Cultures were kept in incubators with 5% CO2 at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... 5 U/mL of FXIII and 1 U/mL of Thrombin were combined in the presence of 10mM Ca2+ with the pelleted µgels and allowed to incubate at 37 °C for 90 minutes between two glass slides (1mm thickness) surface coated with sigmacote (Sigma-Aldrich). The mechanical testing on the hydrogel scaffolds was done using a 5500 series Instron ...
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bioRxiv - Biophysics 2019Quote: Dissected spinal cord tissue was immersed in liquid low-gelling-point agarose (2.5% in aCSF, cooled to 31°C, Sigma-Aldrich, A0701). The tissue was then centered and straightened with insect pins ...
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Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signaturesbioRxiv - Molecular Biology 2019Quote: Testis and epididymis from C57BL/6J mice were fixed overnight at 4°C with neutral buffered formalin (HT501128-4L, Sigma-Aldrich) and embedded in paraffin ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Molecular Biology 2020Quote: ... in a semi-dry blotting chamber for 45 min at 20 V and 4 °C and crosslinked to the membrane with EDC crosslinking solution (127 mM 1-methylimidazole (Sigma-Aldrich), 163 mM N-(3-dimethylaminopropyl)-N&-ethylcarbodiimide hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: ... Samples (lysed samples from ALP activity assay or frozen samples after time point collection) were incubated overnight at 56 °C in Proteinase K solution (1mg*ml-1 Proteinase K (Sigma-Aldrich) in Tris/EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos are recovered at E1.5 or E2.5 by flushing oviducts from plugged females with 37°C FHM (LifeGlobal, ZEHP-050 or Millipore, MR-122-D) using a modified syringe (Acufirm ...