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Citations for Millipore Sigma :
4851 - 4900 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal bovine serum in spinner flasks at 37 °C 10% CO2 atmosphere for 5 days. U2OS (ATCC no. HTB96) were grown in DMEM media (Sigma D6429) with 10% FBS at 37 °C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Microbiology 2024Quote: ... The eluted fractions from solution C (containing target proteins) were applied to an Amicon Ultra 15 Centrifugal Filter (Millipore Billerica MA) for desalting and imidazole removal by using a buffer containing 100 mM Tris–HCl (pH 7.9) ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 ng of trypsin was added to the samples and heated at 37°C overnight with gentle agitation followed by filtration using a 0.22 µm Costar® Spin-X® centrifuge tube filter (Sigma). Samples were then run on a LTQ-Orbitrap-Velos mass spectrometer (Thermo Scientific) ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... subtilis were routinely cultured with shaking at 200 rpm in LB media (Fisher) at 37 °C with 50 mg/mL of kanamycin (Sigma-Aldrich) or 10 µg/mL erythromycin when appropriate ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Immunology 2024Quote: ... Parasites were routinely isolated from lesions of infected BALB/c mice and cultured in M199 medium (Sigma-Aldrich, Darmstadt, He, Germany) supplemented with 0.02% hemin ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Cell Biology 2024Quote: ... One-milligram of lysate was incubated overnight at 4°C with 20 µl of Anti-Flag M2 magnetic beads (Sigma-Aldrich). After extensive washing in RIPA buffer ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for exactly 24 hours at 37 °C in the dark with α-Glucosidase from Bacillus stearothermophilus (0.1 U in 10 μL; Sigma-Aldrich® ...
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bioRxiv - Biochemistry 2024Quote: ... the cells are washed and incubated 45 minutes at 37 °C in extracellular flux analysis medium (sodium bicarbonate-, glucose-, phenol red-, pyruvate-, and glutamine-free modified DMEM [Sigma-Aldrich, catalog no ...
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bioRxiv - Pathology 2024Quote: Nuclei from frozen mouse kidney outer cortex tissue samples and glomeruli-enriched samples were prepared at 4°C.40 Tissue fragments (∼ 8 mm3) were cut by razor blades in EZlysis buffer (#NUC101-1KT, Sigma-Aldrich) and homogenized 30 times using a loose Dounce homogenizer and 5 times by tight pestle ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... 50µg of each protein sample was run on a 10% SDS-PAGE gel followed with a 90V transfer at 4°C for 90 minutes to a PVDF membrane (Millipore #IPFL00010). Membranes were blocked for one hour with Intercept PBS blocking buffer (LiCor #927-40000 ...
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bioRxiv - Systems Biology 2023Quote: ... 50 μM and 100 μM compound for exactly 5 minutes at 25 °C in a thermocycler (Biometra TRIO) before proteinase K (Proteinase K, Tritirachium album, 10 mg, Sigma Aldrich) digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were rapidly harvested and either snap frozen in liquid nitrogen and stored at −80°C for later biochemical analyses or processed by overnight immersion in freshly prepared fixative: 3.7% Paraformaldehyde (Sigma-Aldrich, 441244) 200 mM HEPES ...
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bioRxiv - Genetics 2023Quote: ... The embryonic cell line (ISE6) was cultured at 34°C in L15C300 medium supplemented with 10% heat inactivated fetal bovine serum (FBS, Millipore Sigma), 0.1% bovine lipoprotein concentrate (LPPC ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were spun down at 4,000 x g at 4°C for 10 minutes before being resuspended in PBS containing 1X Bugbuster lysis reagent (Millipore Sigma). Suspensions were incubated on ice for 30 minutes before being spun down at 15,000 x g at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then incubated at 37°C for 30 m with addition of 1 ml of 200 μg/ml Proteinase K (Sigma-Aldrich). The total contents of each digest were then applied to a Qiashredder column (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged (500g at 4°C for 4 minutes) and resuspended in PBS containing 1 μg/mL DAPI (Sigma Aldrich, D9542-100MG). Analysis of flow cytometric data was preformed using the BD Fortessa X20 and FlowJo v10 software (Ashland) ...
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bioRxiv - Neuroscience 2023Quote: ... in a 10×10×5mm cryomold (Tissue-Tek #4565) and submerged in dry-ice chilled (approximately −70°C) isopentane (Sigma #M32631). Frozen embedded brain tissues were kept on dry ice until being transferred to −80°C storage.
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were cultured under sterile conditions at 37°C and 5% CO2 in Roswell Park Memorial Institute-1640 (RPMI1640) (Sigma-Aldrich) for leukemic cells or Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Genomics 2022Quote: ... Tagmentation reaction was performed at 55°C for 30 minutes and stopped by adding 11 μL 2X Stop buffer (40 mM EDTA, 1 mM Spermidine (Sigma, S0266)) to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then pre-hybridized for 20 minutes at 37°C in wash buffer (2x SSC, 10% formamide (Sigma-Aldrich, F9037)) ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Immunology 2023Quote: PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). Cells were then counted and diluted in RP10 to a concentration of 75,000 live cells/mL ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-L-Lysine and Laminin coating of 24-well plates was performed by 48 h incubation at 37°C using Tissue Culture water (Sigma, W3500) supplemented with 1 μg/ml Poly-L-Lysine (Sigma ...
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bioRxiv - Pathology 2023Quote: ... and incubated for 45 minutes at 37 °C in high glucose Gibco™ DMEM containing collagenase XI (0.7 mg/mL; C7657, Sigma-Aldrich) and type IV bovine pancreatic DNase (30 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... The human embryonic tissues were dissociated for 50 minutes at 37°C with 10 mg/ml Collagenase/Dispase (Sigma Aldrich, 10269638001) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... briggsae ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 400 μl with (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: The anterior lobe of freshly dissected 8 to 12 week-old pituitaries was minced and incubated for 15mn at 37°C in 1mg/ml Papain (Sigma 10108014001), 10μg/ml DNAse I (Sigma 10104159001 ...
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bioRxiv - Systems Biology 2022Quote: ... For urine samples a 1:5 (v/v) dilution was prepared in water prior to a urease digestion at 37°C for 40min with 10U urease (Sigma Aldrich). For each sample ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were cut at 350 μm thickness and allowed to recover for ~30 min at 34 °C in standard aCSF composed of: NaCl (120 mM, Sigma-Aldrich), KCl (3 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Expression vectors from previous studies were used to label EGFR at the C-terminus with EGFP and mCherry.(47) These vectors were expressed in COS7 cells purchased from Sigma Aldrich. COS7 cells were cultured in DMEM (Calsson Lab ...
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bioRxiv - Microbiology 2023Quote: ... Ibidi µ-Slide 0.2 channel slides that had been treated at 37 °C overnight with either 100 µL chondroitin sulfate A (100 µg/mL; CSA; Sigma Aldrich) in 1x PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were resuspended and a homogeneous aliquot of 40 uL was hydrolyzed at 37 °C overnight using a mix 110 uL of µL amyloglucosidase (from Aspergillus niger, Sigma, 10102857001) + α-amylase (from pig pancreas ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumour specimens were excised post- mortem and either kept at − 80 °C for RNA extraction or fixed in 1% Paraformaldehyde (Sigma Aldrich) for paraffin embedding.
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bioRxiv - Microbiology 2023Quote: ... pneumophila strains were routinely grown from frozen stocks stored at-80 °C on CYE (ACES-buffered charcoal yeast extract) agar plates (Sigma-Aldrich) adjusted to pH 6.90 with KOH and supplemented with 0.25 g/L L-cysteine and 0.4 g/L ferric pyrophosphate and incubated at 37 °C for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration was performed by ultrafiltration (4 °C, 3200 × g, 100-120 min) using an Amicon Ultra (MW 3,000, Millipore, Cork, Ireland). The medium change to FBS-free Opti-MEM I was required to concentrate the culture medium by ultrafiltration because the viscosity of FBS hampered the ultrafiltration.
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bioRxiv - Biophysics 2023Quote: ... in the presence of horseradish peroxidase (HRP) (stored at 1000 U/ml at -20 °C, thawed on the day of use and kept on ice; Sigma Aldrich) at a final concentration of 2 U/mL ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... one with no AmB and others containing different concentrations between 50 µg/ml and 250 µg/ml added to medium cooled to below 50 °C from a 250 mg/ml stock in water (Sigma-Aldrich) aliquoted to reduce freeze-thaw cycles and stored at -20°C ...