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Citations for Millipore Sigma :
4501 - 4550 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Medium was removed and cells were incubated for another hour (total infection time: 2 h) at 37 °C in FBS-containing culture medium supplemented with 20 μg/mL gentamicin sulfate (Sigma-Aldrich), to kill extracellular bacteria ...
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bioRxiv - Microbiology 2019Quote: ... Each set of matched cultures was either treated for 4hr at 37°C with compound (50nM GNF179, 500nM Chloroquine or 5µM Brefeldin A (Sigma Aldrich #B6542)) or an equal volume of DMSO ...
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bioRxiv - Biochemistry 2019Quote: ... Freshly purified CsgA was filtered using a 30 kDa cut-off column at 4°C (Amicon® Ultra-4, Sigma-Aldrich) to remove insoluble protein aggregates and seeds ...
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bioRxiv - Genetics 2020Quote: ... cells were fixed at day 4 of differentiation with 4% paraformaldehyde for 20 minutes at 4°C followed by permeabilization with 0.1% triton™X-100 (Sigma Aldrich) for 30 minutes at room temperature and blocking in 1xPBS with 1% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... or c) conditioned medium from MMTV-PyMT;Ccr2+/+ cancer cells cultured (either with or without CCR2 antagonist (RS504393, 10 μM, Sigma-Aldrich). After incubation with conditioned medium for 24 h ...
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bioRxiv - Immunology 2020Quote: ... cells were permeabilized for 15 minutes and incubated for 1 hour at 37°C in DNase I (300ug/ml PBS, Sigma Aldrich). After washing the staining procedure for EdU was done according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and then the cells were cultured in 0.4% FBS RPMI-1640 medium with 1 μg/ml mitomycin C (Sigma, cat. M4287) to prevent cell division ...
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bioRxiv - Cell Biology 2021Quote: ... Elution of the co-immunoprecipitated complex was performed by gentle shake of the beads at 4°C in elution buffer supplemented with 3X FLAG peptide (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Reagents used in the organ culture experiments are: Phosphatidylinositol-specific Phospholipase C from Bacillus cereus (PI-PLC, 0.5 U/ml, Sigma-Aldrich P5542), Recombinant Mouse sFRP-1 Protein (1 µg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAi colonies for disl-2 and EV control were grown overnight at 37°C with constant shaking in Luria broth with 100 μg/ml ampicillin (Sigma-Aldrich). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was pre-cleared with agarose beads (1 h, 4 °C) and incubated with 50 µl of saturated anti-V5 beads (Sigma A7345) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... they were incubated in the washing buffer under agitation (50 rpm) at 4°C for 1 hr with 1 μg/mL of rabbit anti-Flag- (SIGMA, F7425) or mouse anti-SUMO-1 (21C7 from the Developmental Studies Hybridoma Bank ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Biophysics 2021Quote: ... The oxidized form of cyt c was obtained by incubation with 5-fold molar amount of potassium hexacyanoferrate (III) (Sigma-Aldrich) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... Lysed scaffolds after ALP assay and frozen films after collection were incubated overnight at 56 °C in Proteinase K solution (1 mg×ml−1 Proteinase K (Sigma-Aldrich) in Tris/EDTA buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Absorption was measured at 525 and 595 nm on a spectrophotometer27 and the ratio was used to calculate the concentration with a chondroitin sulphate C (Sigma-Aldrich) standard curve.
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bioRxiv - Bioengineering 2019Quote: ... Both fibroblast cell types were subsequently growth arrested by incubating with 1 μg/mL mitomycin-C (Sigma- Aldrich, St. Louis, MO) in culture medium for 4 hours prior to detachment from the culture substrates using trypsin as previously described 7 ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized cells were clarified for 20 minutes at 21,000 x g at 4°C and incubated for 1 hour with 1 mL bed volume of anti-FLAG M2 affinity gel (Sigma A4596) which had been pre-equilibrated in DBC wash buffer (0.3% DBC ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Biophysics 2021Quote: ... Cardiomyocytes were attached to imaging chambers (laminin-coated coverslips attached to acrylic slides) for 2 hours at 37°C and fixed in 2% paraformaldehyde (Sigma-Aldrich) (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were incubated at 37 °C overnight in a 1.5% BSA solution with anti-β-III-tubulin (anti-rabbit, 1:500, Sigma-Aldrich) and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed during 5 minutes in PBS and permeabilized overnight at 4°C in 5% normal goat serum (NGS, Sigma Aldrich) and 1% Triton-X100 in PBS ...
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bioRxiv - Genomics 2021Quote: mESCs were grown at 37 °C under a 95% air and 5% CO2 atmosphere on gelatin in serum/LIF medium composed of GMEM (Sigma, G5154) supplemented with 15% FBS (HyClone™) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... cured 16 hours at 65°C) that had been incubated with a mix of 50 μg/mL unlabeled fibronectin (Sigma Aldrich) and 10 μg/mL Alexa405 or Alexa647 (both from Invitrogen)-conjugated fibronectin ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with DMEM-BSA and imaged in a Live Cell System chamber (Pathology Devices) at 37°C with 5% CO2 in the presence of 50 μg/ml PI (Sigma-Aldrich) with or without 50 µM blebbistatin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were added to coverslip chambers containing mono-biotinylated Fab’ goat anti-IgM+G tethered to PLB and imaged at 37°C with 5% CO2 in the presence of PI (50 µg/ml, Sigma-Aldrich) using a TIRF microscope (NIKON Eclipse Ti-E TIRF ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed twice with SFUM to remove surface transferrin and incubated at 37°C with SFUM containing 1mg/mL unlabelled holo-transferrin (Sigma-Aldrich) (pulse-chase ...
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bioRxiv - Cell Biology 2020Quote: ... was then added to a 35mm MatTek coverslip dish (Cat# P35G-0.170-14-C) that had been pretreated by coating with 20 µL of 1 mg/mL solution of soybean lectin (Sigma L1395) in deionized water ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were cultured at 37°C/5% CO2 and glial growth was inhibited at DIV4 by addition of 5-fluoro-2’-deoxyuridine (30 μM)(Sigma, F0503). A 50% fresh culture medium change was performed every 4 days.
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed in 60% isopropanol and incubated for 30 minutes at 37°C in a freshly filtered solution of 3 mg/ml oil red O (O0625, Sigma Aldrich) in 60% isopropanol ...
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bioRxiv - Physiology 2020Quote: ... The tube was centrifuged (340 × g for 5 min at 24°C) and the supernatant was seeded on to 2 separate pre-gelatinised (0.2% in dH2O; Type A, Sigma-Aldrich, UK) T25 flasks (Nunc™ ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Disulfide bonds were reduced by incubation for 45 min at 57 °C with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Catalog no C4706, Sigma Aldrich). The gels were washed and a final concentration of 20 mM iodoacetamide (Catalog no I6125 ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were eluted with 2x Laemmli sample buffer and heated at 95°C for 5 min in the presence of β-mercaptoethanol (Sigma). Whole cell lysates or purified protein samples were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2021Quote: ... 1.4 mL of a vitamin solution [105] and 1.4 mL of 20% (v/v) Antifoam emulsion C (Sigma, St. Louise, USA). During the fermentation 0.5 mL extra antifoam was added when necessary ...
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bioRxiv - Microbiology 2020Quote: ... The 350 pb PCR product was digested and ligated into chloramphenicol-resistant pACD4K-C vector provided by the manufacturer (Sigma-Aldrich). The resulting recombinant pACD4K-C vector was transformed into Escherichia coli DH5a by heat shock ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins from the supernatant were immuno-precipitated for 90 mins at 4°C with FLAG-M2 agarose beads (Millipore Sigma) and washed three times in the lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... 200 μl of bacterial suspension was added to 400 μl of pre-warmed at 37°C human sera (Sigma-Aldrich S7023), and the mixture was incubated for 2h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... DRG tissues were digested and incubated for 21 minutes at 37°C in a solution containing 1 mg L-cysteine (Sigma-Aldrich), 60U Papain (Worthington Biochemical Corporation) ...
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bioRxiv - Neuroscience 2020Quote: ... HT22 cells were incubated at 37 °C and 5 % CO2 in Dulbecco’s modified Eagle’s high glucose medium (DMEM; Sigma-Aldrich, Munich, Germany) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated at 34°C for 72 hours and the cells were fixed with 38% formaldehyde (#47608; Sigma-Aldrich) in a 0.15M saline solution ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were adjusted to 1-2 mg total protein in 1 mL and incubated overnight at 4°C with 25 µL of packed Monoclonal Anti-HA Agarose (Sigma-Aldrich). Following incubation ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were passaged onto Human foreskin fibroblasts (HFFs; ATCC, SCRC-1041) monolayers at 37 °C and 5% CO2 in DMEM (Sigma, D6546) supplemented with 10% FBS (BioSell FBS.US.0500) ...
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bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and all samples were subjected to immunoprecipitation overnight at 4 °C using anti-HA agarose beads (Sigma, A2095). Samples were washed tow times with IP/MS buffer and two times with water ...
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bioRxiv - Microbiology 2021Quote: ... The infected host cells were incubated for 7 min at 37°C with DMEM containing 3 μM of calcium ionophore A23187 (C7522, Sigma-Aldrich) prior to fixation with 4% PFA ...