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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the ligands and small molecule inhibitors used in this study and their concentration were: LIF (1,000 units/ml, Millipore ESG1106), BMP4 (10 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... who were injected intraperitoneally with either poly I:C (POL; P1530-25MG polyinosinic– polycytidylic acid sodium salt TLR ligand tested; Sigma Aldrich) (5mg/kg ...
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bioRxiv - Plant Biology 2019Quote: ... followed by injection of the ligands (50 – 100 μg ml−1) in 10 mM sodium acetate pH 5.0 (Sigma, Germany) until the desired density was reached ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were then treated with 50 nM TMR-conjugated Halo ligand in growth media growth media (DMEM with 10% FBS, 4.5 g/l Glucose (Sigma, G8769), 1x GlutaMAXTM (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with ligands (10 nM DAMGO, 30 nM morphine) and 2.5 μM Forskolin (FSK, Sigma-Aldrich F6886), followed by continuous luminescence imaging for 10 min ...
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bioRxiv - Genomics 2023Quote: ... Subsets of male and female mice on HFD were additionally injected intraperitoneally with the estrogenic ligands 17β-estradiol (E2, 0.5mg/kg body weight, Sigma-Aldrich), 4,4’,4’’-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT ...
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bioRxiv - Cell Biology 2024Quote: ... the depletion of degron-tagged proteins was conducted for l.S hours in the presence of 82.S nM Nocodazole and each specific degradation-triggering ligand (NuplS3: 2S0 nM dTAG-l3 (SML260l, Sigma) & S00 nM dTAGV-l (69l4 ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine assays were performed by the Forsyth Multiplex Core (Cambridge, MA, USA) using the Human Cytokine/Chemokine Magnetic Bead Panel (Milliplex, Millipore Sigma, Burlington, MA, USA) and Bio-Plex®200 plate reader following manufacturers’ specifications ...
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bioRxiv - Immunology 2019Quote: ... Serum and lung immune factors were analyzed by multiplex technology (performed by Eve Technologies, Calgary, AB, Canada) using a 31 cytokine/chemokine/growth factor kit (Millipore, St Charles, MO, USA). The assay was run on a Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay (EMD Millipore Corporation, Billerica, MA, USA). and custom FAS panel on the Luminex MAGPIX ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
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bioRxiv - Animal Behavior and Cognition 2024Quote: Plasma and dorsal hippocampal CA1 cytokines were assessed using a MILLIPLEX® Rat Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma Cat. No. RECYTMAG-65K) customized for detection of pro-inflammatory (TNF-α ...
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bioRxiv - Neuroscience 2023Quote: Inflammatory proteins were measured using the MILLIPLEX Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex – Immunology Multiplex Assay (EMD Millipore. Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Cancer Biology 2019Quote: Immunoblots were performed as previously described [23] using 5% milk powder for blocking and developed using ECL (Sigma-Aldrich). Details of the antibodies used in Additional file 1 ...
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bioRxiv - Neuroscience 2019Quote: ... From DIV 9-23 onwards the medium was supplemented with 2,5% Fetal Bovine Serum (FBS; Sigma-Aldrich #F2442-500ML) to support astrocyte viability.
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bioRxiv - Physiology 2021Quote: ... Adipogenic media as previously described (23) with the modifications of substituting 1μg/mL Troglitazone and 0.25 mM IBMX(Sigma Aldrich) for days 1-3 ...
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bioRxiv - Plant Biology 2020Quote: ... An aliquot of 967 µL was added to 33 µL of 5% w/v Direct Red 23 (Sigma-Aldrich) (1 in 30 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated by adding a non lysing formulation of 0.5 mM D-Luciferin substrate (23) (Sigma-Aldrich) and measuring luciferase activity for 1 second on a plate Reader Infinite 200 PRO (Tecan®) ...
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bioRxiv - Immunology 2021Quote: ... and growth factors were quantified in plasma using a 23-plex MAP NHP immunoassay kit (PCYTMG-40K-PX30, Millipore) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... containing 1% (w/v) of poly(vinyl acetate-co-alcohol) (PVA; MW 13–23 kDa, 98% hydrolysed; Sigma-Aldrich) as stabiliser ...
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bioRxiv - Plant Biology 2024Quote: ... The leaf tissue was cleared in ClearSee at least 24-72 h with gentle agitation and stained with 0.1% Direct Red 23 (Sigma) in ClearSee overnight ...
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bioRxiv - Biochemistry 2020Quote: Gene expression of endogenous CENP-C was inhibited using a single siRNA (target sequence: 5’-GGAUCAUCUCAGAAUAGAA-3’ obtained from Sigma-Aldrich), which targets the coding region of CENP-C mRNA ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at 4°C with anti-BUB-1 (Desai et al., 2003) and anti-tubulin (1/400, clone DM1α, Sigma Aldrich) in AbDil ...
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bioRxiv - Cancer Biology 2021Quote: ... culture medium was replaced with serum-reduced medium (DMEM supplemented with 0.5% FBS) supplemented with 0.02 mg/ml mitomycin C (Sigma, St. Louis, MO), which inhibits cell proliferation ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were incubated for 2h at 4°C with 100 ng of the different GST-V5-CD95 constructs and then overnight at 4°C with 10 µl of Anti-V5 Agarose Beads (Sigma-Aldrich). After extensive washing in Hepes buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Thereafter, cells including their supernatant were harvested and counted using standardized hemocytometers (C-Chip, Biochrom) and the Trypan-Blue (Sigma-Aldrich) exclusion method as described in51.
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bioRxiv - Cell Biology 2020Quote: ... or human NUP153-specific shRNA lentivirus particles overnight at 37°C followed by selection in medium containing Puromycin (Puro, 2 μg/ml) (Sigma-Aldrich) for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 mg of total proteins from whole cell extracts were immunoprecipitated overnight at 4°C using GFP-conjugated magnetic beads (Sigma-Aldrich), then beads were washed three times with immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2019Quote: ... Flies were fattened with dry active yeast for 48 hours at 18°C and then dissected in Halocarbon oil 700 (Sigma-Aldrich) as previously described9 ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: ... JW18 Drosophila melanogaster cells with and without Wolbachia wMel were grown at 24 °C in Shields and Sang M3 insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Developmental Biology 2021Quote: ... Lineage-labeling was induced at indicated time points using fresh or pre-heated (65°C for 10 min) 4-Hydroxytamoxifen (4-OHT) (Sigma H7904) stock solutions in DMSO at a final concentration of 10 μM in E3 embryo medium (Felker et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... testes were incubated at 32 °C for 25 min in 6 mL of Gey’s Balanced Salt Solution (GBSS; Sigma-Aldrich G9779) containing 1.2 mg/mL of Collagenase Type I (Sigma-Aldrich C0130) ...
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bioRxiv - Developmental Biology 2021Quote: ... were designed to generate a G-to-A change creating the R79Q point mutation as well as A-to-C and T-to-A silent changes to create a NdeI restriction site that could be used for genotyping (Millipore Sigma). The gRNA (50 ng/ul) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated in a humidified atmosphere of 5% CO2 in air at 37°C for 2 hours in a 200 μL drop of TYH medium covered with paraffin oil (Sigma-Aldrich) for sperm capacitation ...
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bioRxiv - Microbiology 2021Quote: Drosophila Schneider line 2 (S2) cells were maintained and passaged at 25°C in Shields and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum.
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bioRxiv - Molecular Biology 2020Quote: Uh strains were cultured at 22°C in complete medium (CM) [22] with appropriate antibiotic when needed: 5 μg/ml of Carboxin (Sigma-Aldrich) or 40 μg/ml of Zeocin (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated to 50°C for 15 min before being loaded on 12% TruPAGE Tris-Glycine Precast gels (Sigma Aldrich). Each assay was triplicated ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were cultured at 37°C overnight and induced by addition of 0.2 mM isopropyl-1-thio-β-d-galactoside (Sigma-Aldrich) for 4 hours at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then incubated in LB for 3 hours at 37 °C and plated in LB agar with Kanamycin (Sigma-Aldrich) for 24 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Zirconia beads were pelleted by centrifugation at 21,000xg for 10 min at 4° C and supernatant was transferred to an RNase-free tube containing 100 μL chloroform (Sigma Aldrich). Samples were vortexed for 15 s prior to a 10 min RT° C incubation ...
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bioRxiv - Cell Biology 2021Quote: ... Infection of keratinocytes was done at 15% cell confluence with incubation at 32°C for 1.5 hours in M154 media containing 4 μg/ml polybrene (Sigma-Aldrich, H9268) and retrovirus supernatants ...
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bioRxiv - Molecular Biology 2021Quote: ... so the samples were diluted with lysis buffer without inhibitors and incubated overnight at 4°C with 30 μL of anti-FLAG agarose-coupled beads (#A2220, Sigma-Aldrich) under mild agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was performed completely at 37°C for 18 hours then stopped the reaction by removing enzymes with Amicon® Ultra-15 Centrifugal Filter Unit (Millipore). The product was purified by reverse phase HPLC ...
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bioRxiv - Developmental Biology 2021Quote: ... ChIP and input samples were incubated for 2 hours at 37°C with 0.2 mg/ml RNAse A (Millipore 70856-3), and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Developmental Biology 2021Quote: ... and mounted on a glass-bottom Petri dish (MateTek, P35G-0-14-C) with 1.2% UltraPure low melting-point agarose (Sigma Aldrich, 16520). The injection solution was prepared as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... specimens were dehydrated in an ethanol series at RT and stored in 70% ethanol at 4°C and were embedded in Araldite resin (Sigma-Aldrich). Semithin (1 μm ...