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Citations for Millipore Sigma :
4501 - 4550 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and 3% hydrochloric acid followed by sample pooling via centrifugation at 1,500 rpm for 2 minutes to the proteoCHIP funnel part for direct injection or transferred to 0.2 mL PCR-tubes coated with 1e-3 % Poly(ethylene glycol) (95172-250G-F, Sigma-Aldrich, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... Products from the initial PCR of these samples were then purified using the Ultrafree-DA centrifugal filter units (Millipore, Cork, Ireland); the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... To construct pKD1 the NUP188 coding sequence was amplified by PCR and subcloned into p3XFLAG-CMV™-10 vector (Millipore Sigma) using the Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products with the coding sequence were cloned into NdeI/XhoI or SalI/XhoI sites of pET30a (+) vector (Novagen, Darmstadt, Germany), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... CDC50.3 and CDC50.4 were generated using a PCR fragment encoding the mAID–HA and the HXGPRT cassette produced using the KOD DNA polymerase (Novagen, Merck) with the vector pTUB1:YFP-mAID-3HA as template and the primers indicated in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich). Catalytically inactive Mt2 (Mt2 C78A ...
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bioRxiv - Microbiology 2020Quote: ... The 350 pb PCR product was digested and ligated into chloramphenicol-resistant pACD4K-C vector provided by the manufacturer (Sigma-Aldrich). The resulting recombinant pACD4K-C vector was transformed into Escherichia coli DH5a by heat shock ...
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bioRxiv - Microbiology 2020Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) (Fig 4A) ...
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bioRxiv - Microbiology 2020Quote: ... Double-stranded DNA templates for each sgRNA were produced by template-free PCR with two partially overlapping oligos (PAGE-purified, Sigma-Aldrich). Where necessary ...
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bioRxiv - Neuroscience 2019Quote: cDNAs templates were synthesized from extracted total RNA using omniscript reverse transcriptase (Quiagen) and used to perform PCR amplification under the indicated conditions using Taq DNA polymerase (Sigma-Aldrich).
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bioRxiv - Plant Biology 2021Quote: Seeds were sown individually inside PCR tubes filled with 1X MS gel medium: 0.44% Murashige and Skoog (MS) Basal medium (Sigma-Aldrich, M5519), 0.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... All progenies of genetic crosses were verified by diagnostic PCR using KOD XTREME Hot Start DNA Polymerase (71975-3, EMD Millipore) or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L ...
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bioRxiv - Biochemistry 2022Quote: ... aeruginosa PAO1 served as a template for the amplification of the genes of interest via PCR using the KOD Xtreme polymerase (Novagen/Merck) and cloning primers containing the restriction sites (Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: The LINC00261 wild type cDNA was PCR-amplified from pENTR/D-TOPO-LINC00261 (gift from Leo Kurian) with KOD Xtreme™ DNA Hotstart Polymerase (Millipore). The resulting PCR product was inserted into pRRLSIN.cPPT.PGK-GFP.WPRE via its appended BshTI/SalI cloning sites ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified in 40 μL reactions with 15-18 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and 500 nM of each primer P1.3 and P2.1 (synthesized by IDT ...
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bioRxiv - Molecular Biology 2019Quote: ... an EcoRI restriction site was removed by performing a PCR on AT1.03 and AT1.03R122KR126K pDRF-GW using KOD polymerase (Merck-Millipore, Burlington, Massachusetts, USA) with the forward primer 5’-ATACTAGTGCTAGCTCTAGACTCGAGTATGGTGAG-3’ and reverse primer 5’-ATAGCGGCCGCTGATCAGCGGTTTAAACTTAAGC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20) ...
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bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
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bioRxiv - Cancer Biology 2019Quote: ... digital PCR was carried out using a Biomark HD (Fluidigm) with a 65bp TaqMan assay for the housekeeping gene RPP30 (Sigma Aldrich) (7) ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Genomics 2020Quote: ... The numbers of amplification cycles needed for library preparation of each sample was then determined by quantitative PCR as follows: 25 µl SYBR Green Jumpstart Taq ReadyMic (Sigma-Aldrich), 5µl PCR product from previous reaction ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Microbiology 2020Quote: Cultures reaching ≥ 1 × 105 cells·ml−1 had 35 ml of the 50 ml volume filtered for identification via 16S rRNA gene PCR onto 25 mm 0.22-μm polycarbonate filters (Millipore, Massachusetts, USA). DNA extractions were performed using the MoBio PowerWater DNA kit (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich). The PCR products were then ethanol precipitated ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic regions containing guide sequences are PCR amplified using paired primers with 25 uL 2x JumpStart Taq Polymerase Ready Mix (Sigma-Aldrich), 1.5 uL 10 uM primer pair (CropSeq_NGS_P7 ...
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bioRxiv - Cell Biology 2021Quote: ... the 5’ sequence of the native promoter and amino acids 1-443 of HEH1 were amplified via PCR (KOD polymerase, EMD Millipore) from genomic DNA using primers encoding the restriction sites BamHI and HindIII ...
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bioRxiv - Cell Biology 2020Quote: ... A 245-base fragment of the end of the mto2 coding sequence was amplified from cps1-191 (containing the mto2S338N mutation) with flanking homology to the pFA6a vector using the ExpandTM High Fidelity PCR system (Millipore Sigma). The vector backbone was amplified from pFA6a-GFP-KanMX6 using Phusion polymerase (New England BioLabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were then extracted and used as template in ~400 bp NGS amplicon generation PCR reactions using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The amplicons were gel-extracted using the Monarch DNA Gel Extraction kits (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Indexing PCR reactions were pooled prior to magnetic bead clean-up with Sera-Mag SpeedBeads ™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that are likely to represent contamination from fresh DNA and libraries were selected with a bead concentration of 1.8X to size select the expected library range of ~300 bp ...
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bioRxiv - Microbiology 2022Quote: ... a first PCR was performed using the templates and primers shown in Table 1 with KOD hot start DNA polymerase (Sigma-Aldrich), with annealing time of 30 seconds at the indicated temperature ...
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bioRxiv - Plant Biology 2022Quote: Conserved regions were amplified in a standard Polymerase Chain Reaction (PCR) using genome-specific primers (Linares et al., 1998; Liu et al., 2019) synthesized commercially (Sigma-Aldrich). These probes were labelled with digoxigenin-11-dUTP ...
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bioRxiv - Neuroscience 2022Quote: ... The genomic region surrounding the CRISPR target site for SOX21 was amplified by PCR (KOD Xtreme Hot Start DNA Polymerase; EMD Millipore), and products were treated with SURVEYOR nuclease (SURVEYOR Mutation Detection Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed following a standard procedure: The reaction tubes were prepared by adding 10 µL of RED Extract (Sigma-Aldrich), 4 µL MilliQ water ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR reaction mixtures were set up with 10 µl of SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich), 2 µl primers (final concentration 1 µM) ...
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bioRxiv - Genetics 2023Quote: ... Our method of genotyping involved PCR amplification across mutation sites using the primers described in Table 1 (purchased from Sigma Aldrich). Then ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Synthetic Biology 2023Quote: ... which can be PCR amplified (Phanta Max Super-Fidelity DNA Polymerase, Vazyme) with a set of primers (ordered from Microsynth or Sigma-Aldrich) to add a unique variable linker ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µL of diluted cDNA was mixed with 12,5 µL ReqTaq® Ready Mix™ PCR reaction mix (Sigma Aldrich, #R2523), 0,5 µL forward primer (0,2 µM) ...
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bioRxiv - Microbiology 2023Quote: ... The cassette corresponding the DHFR-pSAG1-TgAPT1-GFP cassette was amplified by PCR from the plasmid of the same name using primers ML3088/ML3089 with the KOD DNA polymerase (Sigma-Aldrich). This donor sequence was then used to transfected to Pru background ...
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bioRxiv - Developmental Biology 2023Quote: ... Truncated FBF expression constructs were generated by PCR from full-length FBF-1 or FBF-2 and inserted into pETDuet-1 plasmid (EMD Millipore). Full-length C ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Genomics 2022Quote: ... Regions determined to be differential between the Illumina sequencing performed in 2017 and the PacBio sequencing performed in 2020 and 2021 were verified by polymerase chain reaction (PCR) using RedTaq (Sigma-Aldrich) according to standard protocols ...
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bioRxiv - Genetics 2022Quote: ... T4 ligations into multiple cloning site plasmids were performed by amplifying fragments via PCR with Roche Expand High Fidelity Polymerase (Sigma 11732641001) with primers containing 5’ restriction enzyme sequences for digestion following PCR purification ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.