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Citations for Millipore Sigma :
4701 - 4750 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the lipid oxidation and superoxide level assays were conducted according to manufacturer’s specifications (Lipid peroxidation assay kit, Sigma Aldrich Cat#MAK085; Fluorimtric hydrogen peroxide assay kit, Sigma Aldrich Cat#MAK165). Total GSH level of liver tissue was measured using a glutathione colorimetric detection kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were analyzed by gel electrophoresis using a 1% agarose gel in TAE buffer (20 mM acetic acid (Sigma Aldrich, cat#, 695092), 2 mM EDTA (ACROS Organics ...
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bioRxiv - Immunology 2019Quote: ... were designed using the chop-chop website (55) and generated by PCR complementation and amplification of full length ssDNA oligonucleotides (Sigma-Aldrich, Table S3) as described (19) ...
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bioRxiv - Biochemistry 2020Quote: DNA encoding for Pry1 and Na-ASP-2 were PCR amplified and cloned into NcoI and XhoI restriction sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a pelB signal sequence to direct the secretion of expressed protein into the periplasmic space ...
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bioRxiv - Microbiology 2020Quote: Full-length sequences of selected sdAbs were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... An amount of 50 μg of Co-IP sample was transferred to a PCR tube and incubated with 1 μL of benzonase (Sigma, cat. no. E8263) at 37°C for 30 min to shear and digest DNA ...
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bioRxiv - Microbiology 2021Quote: Full-length sequences of selected nanobodies were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... [22]) were used to amplify the 16S V4 variable region in PCR reactions using Kapa HiFi Hotstart ready mix (Sigma-Aldrich, Oakville, Canada) (Tables S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR products were purified and used for one-step isothermal assembly with EcoRV-digested pFLAG-CMV-5.1 (Sigma, St. Louis, MO, USA)32.
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 100,000 nuclei were sorted at a ratio of 70% NeuN+ and 30% NeuN-into a single well of a PCR plate (Sigma-Aldrich, EP0030129504-25EA) containing 50 µL FANS buffer (see Table S8) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted (Spectrum™ Plant Total RNA Kit, Sigma) from male and female floral tissues ...
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bioRxiv - Genetics 2020Quote: Telomerase enzymatic activity was analyzed using the TRAPeze kit (Millipore) per manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... Concentrations of glucose were determined using the GAGO20 kit (Sigma).
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bioRxiv - Systems Biology 2020Quote: ... and the Milliplex 38 Cytokine kit (Millipore, HCYTMAG-60K-PX38).
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bioRxiv - Cell Biology 2020Quote: ... using the Duolink in situ red starter kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration standards (Gel Filtration Markers Kit, MWGF1000, Sigma Aldrich) were separated on similar gradients to estimate protein complex size and included ...
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bioRxiv - Immunology 2021Quote: ... Triglycerides were measured using Triglyceride quantification Colorimetric/Fluorometric Kit (Sigma). For trehalose quantification ...
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bioRxiv - Immunology 2021Quote: ... αKG was measured using a colorimetric assay kit (Sigma Aldrich). All kits were used according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: CTCF ChIP was conducted using Millipore ChIP agarose kit (Millipore). 1×106 crosslinked 416B cells were lysed and sonicated using a Covaris ultrasonicator ...
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bioRxiv - Physiology 2022Quote: Liver triglycerides were measured using triglyceride determination kit from Sigma (catalog n°TR0100 ...
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bioRxiv - Molecular Biology 2020Quote: ... then subjected to nuclei extraction (Nuclei EZ prep kit, Sigma). Genomic DNA was isolated using DNAzoL reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... High Sensitivity Triglyceride Fluorometric Assay Kit from Sigma (MAK264-1KT) and Phospholipid quantification Assay Kit from Sigma (CS0001-1KT)).
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bioRxiv - Immunology 2022Quote: ... Serum glycerol were measured using glycerol assay kit (Sigma, MAK117) and normalized to the weight of body fat.
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bioRxiv - Systems Biology 2020Quote: ... Colorimetric glycerol assays were acquired using a commercial kit (Sigma) where the provided assay powder was resuspended in 40 mL of distilled H2O ...
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bioRxiv - Physiology 2019Quote: Plasma insulin was determined using a commercial kit (Merck Millipore) on a MAGPIXTM Multiplex reader and processed using Bio-Plex ManagerTM MP.
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bioRxiv - Microbiology 2019Quote: ... the KAPA SYBR FAST Universal qPCR kit (Sigma-Aldrich, Sweden), and primers specific to the conserved gtfB and gtfC regions in a Corbett Rotor-Gene 6000 apparatus [29] (S1 Table) ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Cell Biology 2019Quote: Duolink PLA was performed using the Duolink Red Kit (Sigma). In summary ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... TRAP staining was performed with a commercial kit (Sigma-Aldrich), randomly selected 5 fields of view on the pressure side of the mesial buccal root of maxillary orthodontic tooth in rats ...
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bioRxiv - Physiology 2019Quote: ... followed by determining protein concentration using BCA assay kit (SIGMA). Samples were loaded onto SDS-PAGE for protein expression analysis.
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bioRxiv - Developmental Biology 2020Quote: ... Assay was performed as per the kit instruction (DUO92101, Sigma). The antibodies used were AR (Sigma A9853 ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... and protein was quantified using a Bradford assay kit (Sigma) following manufacturers’ instructions.
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bioRxiv - Physiology 2020Quote: ... ChIP assays were performed using an EZ-Chip Kit (Millipore). Chromatin lysates were prepared ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was isolated using the RNA isolation kit (Sigma). After RNase-free DNase treatment ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... were measured using an MDA assay kit (#MAK085, Sigma Aldrich) in hippocampal brain tissue isolated from 23 month old APP/PS1 and NTG mice treated for 14 months with CP2 or vehicle (n = 5 per group) ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were isolated using the NucleiEZ nuclei isolation kit (Sigma), with modifications described in Supplemental Methods ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the Spectrum Total Plant RNA isolation kit (Sigma-Aldrich), at SNM ...
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bioRxiv - Bioengineering 2020Quote: ... For Picrosirius red staining (Picro Sirius Red Stain Kit, Sigma) we soaked the section for 30 min in distilled water ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were developed with Immobilon Western Chemiluminescence Kit (Millipore, #WBKLS0500).
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bioRxiv - Cancer Biology 2020Quote: ... H3K27ac ChIPs were prepared using the ChIP assay kit (Millipore) and H2K27ac antibody (ab4729 ...
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bioRxiv - Cancer Biology 2020Quote: ... or the Duolink flowPLA Orange kit (Cat.#DUO94003; Sigma-Aldrich) were used and the PLA was performed according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Cell viability was assessed using the CCK-8 kit (Sigma) for quantitation of viable cell number in proliferation and cytotoxicity assays ...
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bioRxiv - Biochemistry 2020Quote: ... Incubation with kit-specific secondary antibodies (anti-rabbit MINUS Sigma Cat ...
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bioRxiv - Bioengineering 2021Quote: Bromocresol Purple (BCP) Albumin Assay Kit (Sigma-Aldrich, Merck, Germany) performed as per manufacturer’s instructions with plasma samples diluted 5-fold in ultrapure water ...
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bioRxiv - Cell Biology 2021Quote: ... and blocked with Duolink kit Blocking solution (DUO94001, Sigma-Aldrich). Cells were then incubated with primary antibodies to TRF2 ...
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bioRxiv - Plant Biology 2021Quote: ... Plant RNA purification kit (Sigma-Aldrich Co., St. Louis, MO) were used for total RNA extraction according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... was extracted using the GenElute Bacterial Genomic DNA kit (Sigma). The samples of interest were obtained by lysogenizing S ...