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Citations for Millipore Sigma :
4651 - 4700 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The Magna ChIRP RNA interactome kit (EMD Millipore Corp.) was used ...
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bioRxiv - Immunology 2023Quote: ... Human 80 Plex kits were purchased from EMD Millipore Corporation ...
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bioRxiv - Immunology 2023Quote: ... leukocyte (TRAP) kit (387A) was purchased from Sigma (USA). Human TGF-β1 (AF-100-21C) ...
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bioRxiv - Plant Biology 2023Quote: ... or a Spectrum Plant Total RNA kit (Sigma-Aldrich). Removal of genomic DNA was performed with the Turbo DNA-free Kit (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... myrtillus using Spectrum Plant Total RNA Kit (Sigma-Aldrich). cDNA was synthesized using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and an AST Activity Assay Kit (Sigma-Aldrich, MAK055) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2023Quote: ... and SYBR green master mix kit (Sigma, Aldrich, Germany) respectively according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Signals were detected by an ECL kit (Millipore, USA). Experiments were repeated at least three times ...
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bioRxiv - Biochemistry 2023Quote: ... previously quantified via a human ANGPTL4 ELISA kit (Sigma).
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bioRxiv - Immunology 2023Quote: Total RNA was isolated using the GenElute kit (Sigma) and treated with DNA’ase I ...
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bioRxiv - Microbiology 2023Quote: The High Sensitivity Glucose Assay Kit (mak181, Sigma-Aldrich) was used to determine the glucose content in the WTA sample ...
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bioRxiv - Plant Biology 2023Quote: ... We used Spectrum Plant Total RNA kit (Sigma Aldrich) to extract total RNA ...
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bioRxiv - Plant Biology 2023Quote: ... and Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s instructions including on-column DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... immunoreactive proteins were detected using Immobilon Western Kit (Millipore). Protein bands from each blot were observed by an imaging system (ChemiDoc XRS+ ...
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bioRxiv - Cancer Biology 2022Quote: It is a colorimetric assay kit (Sigma Aldrich MAK211) that contains synthetic catecholamine isoproterenol that stimulates β adrenergic receptors ...
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bioRxiv - Cell Biology 2023Quote: ... The DuoLink In Situ Red Kit (Sigma, cat# DUO92008) was used for PLA and the manufacturer’s protocol was followed ...
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bioRxiv - Cell Biology 2023Quote: ... The Anti-human IGFBP2 ELISA kit (Sigma, #RAB0233-1KT) was used to quantify IGFBP2 protein in the supernatants according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... DNA was visualized using the Immobilon Western kit (Millipore) and photographed by a Tanon 5200 Multi-Imaging System (Tanon ...
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bioRxiv - Microbiology 2023Quote: ... The Complete Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich) was employed with minor adaptations (94°C for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... Protein was estimated using BCA kit (Sigma Aldrich, USA) and 20 µg protein was loaded on 12% SDS-PAGE gel ...
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bioRxiv - Microbiology 2023Quote: ... or the Free Glycerol Determination Kit (FG0100, Sigma USA). In addition to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and gel purified using GenElute Gel Extraction Kit (Sigma) according to manufacturer’s guidelines and eluted in 50 μl of kit elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Elutions were precipitated using ProteoExtract Protein precipitation kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... mouse GLP-1 ELISA kit (EZGLP1T) from Sigma-Aldrich; mouse GDF15 ELISA kit (DY6385 ...
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bioRxiv - Microbiology 2023Quote: ... using a SeqPlex DNA Amplification Kit (Sigma-Aldrich, USA), which is specifically designed for low-quantity ...
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bioRxiv - Neuroscience 2023Quote: ... using Nuclei Pure Prep Nuclei Isolation Kit (Sigma Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2024Quote: The lactate dehydrogenase colorimetric assay (Abs450) kit (Sigma, MAK066) measures LDH activity in a biological sample based on the reduction of NAD to NADH ...
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bioRxiv - Cell Biology 2024Quote: ... using the GenElute™ HP plasmid MidiPrep kit (Sigma). Plasmids for expression of ERp44wt and C29S mutant were a kind gift of Roberto Sitia.
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bioRxiv - Cell Biology 2024Quote: GeneElute single cell RNA isolation kit (Sigma Aldrich, USA) was used for total RNA isolation ...
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bioRxiv - Physiology 2024Quote: ... Masson’s trichrome staining (Trichrome Stain [Masson] Kit, Millipore-Sigma) was performed on adjacent sections as previously described (10).
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bioRxiv - Physiology 2024Quote: ... Masson’s trichrome staining (Trichrome Stain [Masson] Kit, Millipore-Sigma) was performed on adjacent sections as previously described (10).
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bioRxiv - Biochemistry 2024Quote: The GAPDH Activity Assay Kit (Sigma-Aldrich, MAK277-1KT) has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase enzyme after S-cyanylation ...
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bioRxiv - Genomics 2020Quote: ... 1-4 PCR wells were combined in a tube and 40 μL of 97% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich #370533) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... whilst AL-PHA assay plates were centrifuged 500 g for 10 minutes (MPS 1000 Mini PCR Plate Spinner, Sigma-Aldrich, MO, USA) in order to pellet AL-PHA beads ...
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bioRxiv - Neuroscience 2020Quote: ... Templates cDNA fragments for the synthesis of riboprobes were obtained by PCR using specific primer sets (Table 1) and subcloned into the plasmid pSTBlue-1 (Novagen, Madison, WI). Nucleotide sequencing revealed 100% homologies of these fragments to the reported sequences in Genbank ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested and with Nde1/Xho1 restriction enzymes and inserted into the bacterial expression vector pET-30a(+) (Novagen, Madison, WI) cut with the same enzymes ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... A PCR master mix was prepared by combining the following reagent volumes per sample: 15 µl of Platinum PCR Supermix UDG (Sigma Aldrich, USA), 0.25 µl each of Forward and Reverse Primers (10 µM ...
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bioRxiv - Microbiology 2022Quote: ... 500 base pairs of upstream and downstream sequence surrounding PBP-lipo (Rv2864c and MSMEG_2584c) were amplified by PCR KOD XtremeTM Hot Start DNA polymerase (EMD Millipore, Billerica, MA). These flanking regions were amplified with overlaps for the zeocin resistance cassette ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Microbiology 2022Quote: ... K986P and V987P (S-2P) (70–72) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M ...
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genomics 2021Quote: ... we performed up to 8 nested PCR reactions in parallel with an input of 100-200ng per reaction using the KAPA2G Robust ready mix (Sigma-Aldrich, KK5702) in a volume of 50μL ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were visualized by 1.5% agarose gel electrophoresis and purified using a Millipore filter plate MSNU030 (Millipore SAS, Molsheim, France). The purified PCR products were then subjected to Sanger sequencing using the BigDye Terminator v3.1 sequencing kit and a 3730xl automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem, cat# KK2602) or Expand Long Template PCR System (Sigma, cat# 11681842001). Primers used for each PCR reaction are listed in Table S4.
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bioRxiv - Cancer Biology 2023Quote: ... through a 130 μm low-pressure deposition nozzle into 96 well PCR plates containing 10 µL 1x prepared Cell Lysis and Fragmentation Buffer Mix (Millipore Sigma, WGA4). Doublets were excluded from analysis via standard FSC/SSC gating procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).