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Citations for Millipore Sigma :
4501 - 4550 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Biophysics 2024Quote: ... then protein was eluted with elution buffer (wash buffer with 10 mM d-Desthiobiotin (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting loading samples were prepared after adjusting the protein concentration using 5X SDS (Sigma-Aldrich) and denatured by boiling ...
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bioRxiv - Biophysics 2024Quote: ... the protein was further purified by binding to ANTI-FLAG M2 Magnetic Beads (Sigma Aldrich) and eluted with 3× FLAG Peptide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg of nuclear protein was concentrated in 500ul using a Microcon centrifugal filter (Millipore) and then applied to a Superose 6 size exclusion column (10/300 GL GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was estimated using 4% copper sulfate and bicinchoninic acid (Sigma, USA, Cat#B9643). Protein samples were separated by 8-12% SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The amount of total protein in the samples was quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... After electrophoresis proteins were transferred to a Polyvinylidene fluoride (PVDF; Immobilon-P™, Millipore®) membrane using semi-dry blotting (Amersham biosciences TE 77 ECL Semi-Dry Transfer Unit ...
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bioRxiv - Physiology 2023Quote: ... STIM-1 polyclonal (Protein Tech group) was used at 1:250 and HCN4 polyclonal (Sigma) was used at 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
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bioRxiv - Synthetic Biology 2022Quote: ... The buffer of the eluted protein was subsequently exchanged using a G25 column (Sigma-Aldrich) into TEV buffer (50 mM Tris ...
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bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed in protein lysis buffer (Cell Signalling) supplemented with phosphatase (Phos-Stop, Sigma) and protease (Complete Mini ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with a combination of LysC (Wako Chemicals) and Trypsin (Porcine, Sigma Aldrich) (1:70 and 1:25 enzyme to protein ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration in the extracts was measured using the QuantiPro BSA Assay Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized with the FITC- and TRITC-conjugated secondary antibodies (Sigma, F6005, and T5393) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were fractionated by SDS-PAGE and transferred to 0.4 µm PVDF membranes (Millipore, MA) prior to immunoblotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, USA) for 30 min using the Towbin’s transfer buffer in a semi-dry transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of protein was incubated with 20 mM methoxypolyethylene glycol maleimide (mPEG, Sigma-Aldrich) in the presence of 3% SDS for 10 minutes at 60ºC ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was precipitated after chilling samples on ice and adding cold ammonium acetate (Sigma, A1542) to a final concentration of 1.875 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Total proteins of BR-EVs were extracted with RIPA buffer containing 0.5% PMSF (Sigma Aldrich). In brief ...
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bioRxiv - Biophysics 2023Quote: ... Protein was eluted with wash buffer supplemented with 100 mg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to a storage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Microbiology 2023Quote: ... The protein bands were developed with Immobilon Forte Western HRP substrate (Millipore Sigma, MA, USA) and imaged using a Bio-Rad ChemiDoc Imaging System (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Microbiology 2023Quote: ... Dried protein pellets were resuspended with 50 mM TEAB buffer (Millipore Sigma, Burlington, MA, USA) and quantified using Pierce 660nm Protein Assay Reagent (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein’s peak fractions were pooled and concentrated using 50kDa cut-off Amicon (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore).
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was pre-treated with EZview Red Protein A agarose beads (P6486; Sigma) for 45 minutes to reduce the likelihood of nonspecific binding to the agarose ...
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bioRxiv - Cell Biology 2022Quote: Proteins from GL261 and CT2A cells were extracted in RIPA buffer (Sigma, cat# R0278-50mL) containing a protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and the lysates were immunoprecipitated with specific antibody and protein A/G-Sepharose (Sigma-Aldrich) at 4 °C for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was extracted using radioimmunoprecipitation (RIPA) assay buffer (Cat # R0278, Sigma-Aldrich, St. Louis, MO) containing cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed protein was purified using Glutathione Sepharose 4B (Sigma-Aldrich Cat# GE17-0756-01) using the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Western blotting of immunoprecipitated proteins was performed as described above using anti-FLAG (F1804, Sigma) or anti-HA (11867423001 ...
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bioRxiv - Biochemistry 2023Quote: ... and protein thereafter eluted with Strep-buffer supplemented with 2.5 mM d-desthiobiotin (Sigma-Aldrich). All purified proteins were concentrated to appropriate concentration using 30 kDa Mw cut-off centrifugal filters (Amicon ...
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bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...