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Citations for Millipore Sigma :
4301 - 4350 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein samples were concentrated to 30mg/ml using 100-kDa MWCO AmiconUltra concentrators (Millipore) for crystallization trials and further analysis.
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bioRxiv - Cancer Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA, USA).
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Pathology 2022Quote: ... Proteins separated via SDS-PAGE gels were blotted onto PVDF membranes (Millipore, Cat. No. ISEQ00010). To reduce non-specific antibody binding and reduce background ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were subjected to 10% SDS-PAGE and moved to PVDF films (Millipore, America). After the blockade with 5% milk ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: ... the eluted proteins of IP were transferred to a 10 kD centrifugal spin filter (Millipore), and followed by washing with 8 M urea 200 μL for three times ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by 15% SDS-PAGE gel and blotted onto a nitrocellulose membrane (Millipore). The membrane was first stained with Ponceau S to show protein loading before blocking and incubation with first antibodies ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Pathology 2022Quote: ... Equal amounts of proteins were incubated with anti-FLAG M2 affinity gel (Sigma Aldrich, A2220) and processed by Western blotting.
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bioRxiv - Genetics 2022Quote: ... 200 μg of protein extract was incubated with 30 μl anti-HA magnetic beads (Sigma) at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were electrophoretically separated using 10% SDS gel and transferred onto nitrocellulose membrane (Millipore, USA). The membranes were blocked with 5% Non-Fat Dry milk in PBST (1X PBS with 0.1%Tween-20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts from tumor tissues were prepared by homogenization in RIPA buffer (Sigma-Aldrich, USA) containing a protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Cancer Biology 2022Quote: Total protein extracts were obtained from cell lines and tumour specimens using RIPA (Sigma-Aldrich) buffer and subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were stained with Ponceau S (0.1% Ponceau reagent [Sigma, P3504] with 5% acetic acid). Incubation with primary antibodies was conducted overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich, St. Louis, MO, USA) and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were reduced with 60 µL 40 mM dithiothreitol (DTT; Sigma-Aldrich, Taufkirchen, Germany) in 50 mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Cell Biology 2021Quote: ... crosslinked to Protein A/G PLUS-Agarose (SantaCruz) using 20 mM dimethyl pimelimidate (Sigma Aldrich) at 4°C for overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into REMSA buffer [10mM HEPES pH 7.3 (Sigma-Aldrich, Cat. No. H3375), 20 mM KCl (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then electro-transferred onto a polyvinylidene fluoride (PVDF) membrane (0.2 µm; Millipore). After blocked with 5 % skim milk in TBST (50 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Microbiology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (#16-663, Millipore) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Chloroquine diphosphate (C6628) and HRP-conjugated Protein G (#18-160) were purchased from Sigma-Aldrich. Torin1 (#4247 ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... separated protein lysates were transferred onto polyvinylidene difluoride membranes (Immobilon-P; EMD Millipore, Burlington, MA); membranes were blocked in 5% BSA (BSA Fraction V ...
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bioRxiv - Neuroscience 2021Quote: ... and the proteins denatured by adding 200 μL of 8 mol/L urea (Sigma-Aldrich), followed by 15 min sonication ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biophysics 2020Quote: ... The purified protein was incubated and for 1h at 37°C with bovine thrombin (Sigma) in a 1000:1 molar ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (16-663; Millipore) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... fractions containing pure protein were concentrated using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma), dialyzed into PBS ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of total protein in the samples were quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer and the samples were boiled for 10 min at 100° C ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was transferred from the 20% polyacrylamide gels to PVDF Immobilon-P membranes (Millipore Sigma) and blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were transferred into new tubes and protein concentration were determined by BCA assay (Sigma). Approx ...