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Citations for Millipore Sigma :
4451 - 4500 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Biochemistry 2022Quote: ... protein was concentrated in an Amicon Ultra-15 30 kDA centrifugal filter units (Millipore #UFC9030) and incubated overnight at 4 °C with His-tagged PreScission protease (purified in house ...
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bioRxiv - Biochemistry 2022Quote: hnRNPDL proteins were detected using different primary antibodies (anti-hnRNPDL antibody, HPA056820 from Sigma-Aldrich or with an anti-GFP monoclonal antibody ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% TritonX-100) containing protease and protein phosphatase inhibitor cocktails (Sigma Aldrich, Gillingham, Dorset, UK). Protein concentrations were measured with BCA protein assay and samples were boiled for 5 min at 90°C after addition of 5%β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins bands were immobilized through transfer to PVDF membrane (IPFL00010, Immobilon Transfer Membrane, Millipore, Ireland) for one hour at 110 volts in ice ...
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bioRxiv - Cell Biology 2022Quote: ... the enzymatic activity derived from the fusion protein was detected using NBT/BCIP substrate (Sigma). For competition binding ...
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bioRxiv - Genomics 2022Quote: mECSs harboring a homozygous endogenous NELFB-FKBP12F36V fusion protein were cultured on 0.1% gelatin (Millipore) in PBS+/+ coated tissue-culture grade plates ...
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bioRxiv - Plant Biology 2022Quote: ... Elution of immunoprecipitated proteins was performed using 100 μg/mL c-Myc peptide (Sigma, M2435), on ice for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... After adding 1x protein loading buffer (42526.01, SERVA) containing 5% ß-Mercaptoethanol (M6250, Sigma-Aldrich), protein samples were denatured 5 min at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP) (EMD Millipore; AB5804), rabbit anti-microtubule associated protein-2 (MAP2 ...
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bioRxiv - Plant Biology 2022Quote: Protein samples were run on SDS-PAGE and transfer onto PVDF membrane (Immobilon-P, Millipore). The membrane was blocked by PVDF blocking reagent (Toyobo ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Physiology 2022Quote: ... the University of Michigan Transgenic Core injected Cas9 protein (Sigma-Aldrich, St. Louis, MO, USA), editing templates containing LoxP sites (IDT Ltd. ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ECL Western blotting Analysis System was used to reveal protein bands (Millipore, United States). Densitometry was performed with ImageJ software and standardized against β-actin.
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation of FLAG fusion proteins was performed using an anti-FLAG M2 affinity gel (Sigma) by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Supernatant was used as input fractions ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Sample were then mixed with 4x SDS buffer and boiled for 95°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μg protein per sample was alkylated for 30 min with 20 mM Iodoacetamide (Sigma) and digested with 5% trypsin (w/w ...
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bioRxiv - Plant Biology 2024Quote: ... The DDB2-FLAG fusion protein was detected using the anti-FLAG HRP (Sigma-Aldrich: A8592) at a 1:5,000 (v ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized by chemiluminescence using either Immobilon Chemiluminescent HRP substrate ECL (Millipore #WBKLS0500) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher #34095) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated using Amicon 10K and 30K columns (Millipore, Ref. UFC803096; UFC801096) until the final protein concentration reached approximately 1 to 2 mg/mL ...
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2024Quote: ... viral media was collected and passed through a 0.45 μM low-protein binding filter (Millipore) and concentrated by low-speed centrifugation (3300 g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bands were immobilized through transfer to PVDF membrane (IPFL00010, Immobilon Transfer Membrane, Millipore, Ireland) for 90 min at 30 volts in ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were diluted to the same protein concentration using 2X Laemmli buffer (Sigma-Aldrich), boiled for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The final protein was concentrated by using Amicon Ultra 30 kDa MWCO filters (Millipore Sigma).
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bioRxiv - Microbiology 2024Quote: ... the protein was concentrated to approximately 30 mg/ml using an Amicon Ultracel-10K (Millipore) and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were detected and visualized for protein bands with Immobilon Forte western HRP substrate (Millipore) and imaged in Image Quant LAS4000 ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mg lysate proteins were mixed with 200 µL of anti-Flag M2 (Sigma-Aldrich) or anti-HA (Thermo Scientific ...
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bioRxiv - Genetics 2024Quote: ... Proteins were transferred to either NitroBind nitrocellulose (GE, EP4HY00010) or Immobilon-FL PVDF (Millipore, IPFL00010) membranes in Tris-Glycine transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... 5-100 ng of S-RBD protein (SinoBiological, 40592-V08B) or ovalbumin (Sigma-Aldrich, A5503) recombinant protein were added as control ...
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bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Luminol Reagent (Millipore, WBKLS0500) and ChemiDoc XRS+ Imaging system (Bio-Rad) ...