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Citations for Millipore Sigma :
4601 - 4650 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins on the gel were then transferred to Immobilon-FL PVDF membranes (Millipore) at 100V for 1 hr 12 mins ...
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bioRxiv - Genetics 2024Quote: ... proteins were loaded on an 8% polyacrylamide gel and transferred onto a PVDF membrane (Millipore). Chemiluminescence was induced by Luminata Forte Western HRP Substrate (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... Eluted protein was concentrated to 2 mL by centrifugation using Amicon Ultra centrifugal filters (Millipore) with a 50-kDa molecular weight cut-off (MWCO ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell protein lysates were prepared in RIPA buffer freshly supplemented with proteinase inhibitors (Millipore) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... protein expression was induced by adding 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma) and 1 mg/mL riboflavin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Coding sequences for M and Enn proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated with addition of Trichloroacetic acid (TCA) 100% (1/5v/v) (Sigma #T6399) and washed with 0.01M HCl/ 90% Acetone before by peptide identification by LC/MS.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich 68957) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich 22790) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysates were mixed with 30 µL of Protein G Sepharose 4B (Sigma-Aldrich) and rotated at 4°C for 2 h with 2.5 µg of mouse anti-Myc antibody (Calbiochem) ...
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bioRxiv - Molecular Biology 2024Quote: ... Bait-purified prey proteins from plasma were then eluted with 5 mM biotin (Sigma-Aldrich) in ice-cold 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... The proteins in the gel were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA). After protein transfer ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested overnight at 37°C with mass spectrometry grade trypsin (SOLu-Trypsin, Sigma) in an enzyme:substrate ratio of 1:50 ...
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bioRxiv - Physiology 2024Quote: ... The separated proteins were transferred to an Immobilon PVDF-FL membrane (#IPFL00005, Millipore, Billerica, MA) at 100 V ...
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bioRxiv - Pathology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA). The membranes were reacted with the following primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich), and stored in 20 mM HEPES ...
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bioRxiv - Systems Biology 2024Quote: ... and 0.25 ml 10 X endonuclease-free BugBuster Protein Extraction Reagent (Millipore, Cat. No. 70921) was added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein-containing fractions were concentrated using a 3 kDa cutoff Amicon centrifugal filter (Sigma UFC8003) and loaded onto a Superdex 75 size exclusion column equilibrated with a storage buffer (25 mM Na2HPO4 pH 7.0 ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: Protein samples were mixed 1:1 with 2X Lämmli sample buffer (Sigma-Aldrich, S3401-1VL) and denatured at 95°C with shaking at 400 rpm for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: ... the diluted protein solution was carefully mixed with Ficoll-70 (Sigma-Aldrich, Cat# 72146-89-5) or BSA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... The purified protein sample was concentrated in 100 kDa molecular weight cut-off (MWCO) concentrator (Millipore) to 3 mg/ml by centrifugation at 2,500 g.
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bioRxiv - Cancer Biology 2021Quote: ... Separated protein was transferred onto a Immobilon-FL PVDF membrane (Millipore Sigma, Burlington, MA, USA #IPFL00010). Membranes were blocked in 5% milk in TBS with 0.1% Tween-20 (TBS-Tween ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved on 8–12% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Millipore). Subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich, Cat #F1804) or 8 μg anti-U2AF2 antibody (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 30 μg of protein were subsequently labelled with stable-isotope labelled iodoacetamide (heavy IAA, 13C2D2H2INO, Sigma) to alkylate reversibly oxidised cysteines ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were harvested by centrifugation and resuspended in BugBuster® Protein Extraction Reagent (Millipore #70584-4) supplemented with protease inhibitor cocktail (cOmplete EDTA-free ...
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bioRxiv - Cell Biology 2020Quote: Total protein cell lysates were obtained using RIPA buffer containing protease inhibitor cocktail (11697498001, Sigma-Aldrich), phosphates inhibitors (A32957 ...
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bioRxiv - Cell Biology 2020Quote: ... The translated protein was immunoprecipitated using 1.2 mg of anti-Flag coupled magnetic beads (Sigma-Aldrich, #F1804 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 dpf larvae with sparse fluorescent protein labeling of PNs were anaesthetized in 0.001% MS222 (Sigma) for 1 minute ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PAGE under reducing conditions and then transferred onto PVDF membranes (Millipore) for Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... host mice were challenged with 7.5 μg of full-length OVA protein (grade V, Sigma Aldrich) injected into the caudal tail vein ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... except that elution of bound proteins was done using 100 μg/ml FLAG peptide (Sigma, F3290).44 In brief ...
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bioRxiv - Immunology 2020Quote: Equal amounts of protein extracts were separated by SDS-PAGE and transferred to PVDF membrane (Millipore). Bands were detected with enhanced chemiluminescence (ECL ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins samples were then transferred from the acrylamide gel onto a methanol activated PVDF membrane (Millipore) for 1 hour at 100 V ...
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bioRxiv - Microbiology 2021Quote: ... enabling formation of protein-containing polyacrylamide plugs upon polymerization using ammonium persulphate and TEMED (Sigma-Aldrich). Gel plugs were then diced by spinning at 14,000 xg through plastic mesh ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated by 10% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore, Germany), followed by blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Molecular Biology 2020Quote: ... the protein aliquot taken for pull-down was incubated with 10 mg of free avidin (Sigma) per 1 mg of protein input at 4°C for 1 h with constant rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Aliquots with optimal protein concentrations were collected and transferred to PBS (Millipore, Amicon ultra centrifuge filter), supplemented with protease inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated in 1% Western blocking reagent (Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... the proteins were transferred from the gel to a 0.2 μm Immobilon-PSQ PVDF Membrane (Millipore). The membrane was blocked for 1 h at room temperature and incubated overnight at 4°C with CREM/ICER antibody (clone 3B5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
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bioRxiv - Neuroscience 2019Quote: ... Crude protein extracts were prepared by homogenizing the cortices in cold RIPA buffer (Sigma-Aldrich #R0278) containing a protease inhibitor cocktail (Thermo Fisher Scientific #A32955 ...
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bioRxiv - Immunology 2019Quote: ... and the eluted protein was concentrated using the Amicon® Ultra 15 mL 3k device (Millipore) after being dialyzed for 24 h at 4°C against phosphate buffer saline (PBS ...