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Citations for Millipore Sigma :
4551 - 4600 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Zoology 2023Quote: ... 10 μl of supernatant was used for protein quantification using Bradford Reagent (Sigma, B6916-500ML). Whole-body uric acid levels were measured from 5 μl of supernatant at 22°C for 30 min using uric acid assay kit following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was purified using the HiPrep Q Fast Flow 16/10 column (Sigma-Aldrich) followed by the reverse-phase HPLC C4 column ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... Isolated proteins were then segregated by gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). Membranes were subsequently incubated in primary antibodies (Supplementary Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... Purified proteins were further concentrated and changed to PBS buffer using the Centricon device (Millipore).
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bioRxiv - Neuroscience 2023Quote: Total protein samples were lysed in radioimmunoprecipitation (RIPA) buffer containing 2% protease inhibitor cocktail (Sigma). Protein quantification was completed using the Pierce Bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by 10% SDS-PAGE and transferred to the PVDF membrane (Millipore, USA). The membrane was placed in 5% bovine serum albumin and incubated with the anti-midP0188 pAb (1:500 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... TEV digested protein was concentrated to < 2 mL using an Amicon Ultra-15 concentrator (Millipore) and then purified by size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The separated protein samples were transferred to PVDF membranes (Immobilon-P; Millipore, Burlington, MA, USA) using the Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were purified from clarified supernatants using a 1mL StrepTrap™ HP column (Sigma), buffer exchanged ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 0.5ml using Amicon Ultra-4 ml Centrifugal Filters (Millipore) and loaded onto a gel filtration column (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then added with 75 μl pre-washed Protein A Sepharose (Sigma, #P6649) and incubated at 4 °C for 30 min with rotation ...
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bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
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bioRxiv - Biochemistry 2023Quote: ... Polyubiquitinated proteins were visualized by immunoblotting for ubiquitin (Millipore Sigma 05-994, clone P4D1-A11) and actin was used as loading control (Millipore Sigma MAB1501R ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
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bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... beads were incubated directly with 4X protein loading buffer (Li-Cor) with 2-Mercaptoethanol (Sigma). For digital PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were then pooled based on protein concentration and Amicon centrifugal filters (EMD Millipore) were used to concentrate the samples ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were loaded into Amicon® Ultra-15 centrifugal filter unit (Millipore Sigma), washed with Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were detected with ECL Prime Western blotting Amersham (Sigma Aldrich, cat. no GERPN2232) detection reagent and images acquired with STELLA imaging system ...
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bioRxiv - Immunology 2023Quote: ... protein bands within the gel were transferred on an Immobilon-FL transfer membrane (Millipore IPFL00010) for 45 mins at 20 volts and 400 mAMPs ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was eluted with a cocktail of 100 ug/ml RNase A (Sigma-Aldrich) and 0.1 units/ µL RNase H (Epicenter) ...
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bioRxiv - Cancer Biology 2023Quote: ... All the proteins were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore Corporation, UFC901024).
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bioRxiv - Developmental Biology 2023Quote: ... Medium was supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Sigma) when iPSCs were thawed or passaged as single cells.
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bioRxiv - Cell Biology 2023Quote: Protein lysates were resolved in SDS-PAGE gels and transferred to PVDF (Millipore, 0.2 µm) membrane ...
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bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... We used primary antibodies for astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541). To quantify relative intensity of staining for GFAP we measured the average signal intensity in the region of interest and normalized it to that of the WT littermate mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... to stain adrenergic nerve fibers and growth association protein 43 (GAP-43, Millipore, #AB5220;1:100) for regenerating nerve terminals ...
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bioRxiv - Cancer Biology 2023Quote: Equal amounts of protein or EVs were mixed with reduced (containing 5% β-mercaptoethanol, Sigma) or non-reduced 4 x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were transferred onto a 0.45 μm pore polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore), previously activated in cold 100% methanol ...
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bioRxiv - Genetics 2024Quote: ... and the protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore, Merck).
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bioRxiv - Developmental Biology 2024Quote: Proteins were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, IPVH00010) for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins solubilized into serum-containing media were pulled down by using heparin-sepharose beads (Sigma). Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5) ...
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bioRxiv - Molecular Biology 2024Quote: Protein samples were separated on Bis-Tris gels and transferred to a PVDF membrane (Millipore). For immunoblots showing multiple proteins with similar molecular weight ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to probe target proteins with the BCIP®/NBT Liquid Substrate (Sigma-Aldrich). ImageJ software (National Institutes of Health ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing pure polyhistidine-tagged proteins were pooled and concentrated by centrifugation filtration (EMD Millipore; using appropriate MWCO ...
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bioRxiv - Biochemistry 2024Quote: ... The disulfide bridges in the protein extract were reduced in 10 mM dithiothreitol (DTT, Sigma) for 1 h at 37 °C and shaking at 1000 rpm in a TC ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 30K MWCO to 50-100 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 3K MWCO to 100-200 µM ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Plant Biology 2024Quote: Yeast protein was extracted by homogenizing yeast cells with acid-washed glass beads (Sigma-Aldrich) in homogenizing medium (0.25 M sorbitol ...
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bioRxiv - Pathology 2024Quote: ... Proteins were then transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were concentrated to 1 mL with a 30K MWCO concentrator (EMD Millipore) and diluted with 1 mL buffer A ...