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Citations for Millipore Sigma :
401 - 450 of 1005 citations for PCR strip since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The libraries were PCR amplified with KAPA HiFi Hotstart Ready mix (Sigma-Aldrich) and barcoded Nextera custom primers 17 and finally size-selected (250–350 bp ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PCR products were concentrated using Amicon Ultra centrifugal filter units (Millipore) and buffer exchanged against 10 mM HEPES pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035).
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit (Sigma): 50 µl of extraction solution and 12.5 µl of tissue preparation solution was added to each embryo ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were visualized after agarose gel electrophoresis using GelRed® stain (Sigma) and a GelDoc imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: All PCR reactions were carried out using KOD Hot Start DNA polymerase (Novagen). All RT-PCRs were carried out using Takara’s PrimeScript High Fidelity RT-PCR kit (R022A).
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Mycoplasma contaminations were tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, USA). LNCaP abl were subjected to short tandem repeat (STR ...
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bioRxiv - Bioengineering 2022Quote: ... After digestion the PCR product was ligated into the pET30a vector (EMD Millipore). The resulting construct encodes EcSTH with both an N-terminal Hisx6-tag and a C-terminal FLAG-tag.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... It was generated by PCR amplification (DNA KOD Hot-start DNA Polymerase, Novagen) and cloned into the pENTRD-TOPO Gateway vector using the manufacturer’s protocol and confirmed by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were tested for mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was subcloned and inserted into a pET-24b (+) plasmid (Novagen) digested with NheI/XhoI placing the gene upstream of a sequence encoding six histidine residues ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using SYBR green PCR Jumpstart Taq ReadyMix (Sigma-Aldrich), ROX Reference Dye (ThermoFisher Scientific ...
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Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activationbioRxiv - Molecular Biology 2023Quote: ... Sanger sequencing of the PCR products was performed using a commercial service (Sigma). The sequencing traces were examined and the fish carrying A to G mutation were selected ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were screened for mycoplasm using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) or Mycostrip detection kit (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mix was: 0.45 µL DMSO for PCR (Sigma; cat# D8418-50mL), 0.0045 µL SYBR Green I (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
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bioRxiv - Biophysics 2024Quote: ... sample wells (Fig. S14) were made from half-cut PCR tubes (Sigma-Aldrich) glued to the prepared slides with epoxy (Ellsworth Adhesives) ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and verified to be mycoplasma-free using LookOut® Mycoplasma PCR Detection Kit (Sigma). Density gradient purified Chlamydia trachomatis L2/434/Bu (ATCC VR902B ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments generated were cloned into a pET-41 Ek/LIC vector (Novagen) expressing an N-terminally GST-His6-tagged protein ...
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bioRxiv - Microbiology 2020Quote: ... The linear constructs were gel purified using a PCR cleanup kit (Sigma-Aldrich, Australia), confirmed by Sanger sequencing and concentrated to ∼500 ng/μl.
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bioRxiv - Microbiology 2021Quote: ... which was combined with 10µL of KiCqStart SYBR Green PCR Ready Mix (Millipore Sigma), 1µL of forward primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were purified using PCRu96™Plates (Merck Millipore Inc., Burlington, MA) and sequenced by an ABI 3130 (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... Q-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were tested for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma MP0035), following the manufacturer’s instructions and using the JumpStart Taq DNA polymerase
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bioRxiv - Neuroscience 2019Quote: ... The wild-type or T94M mutant ORFs were then PCR-cloned into pET28c (Novagen) for bacterial production of the proteins with an N-terminal 6× His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... The PTBP1 ORF was PCR amplified and subcloned by gibson cloning into pET21A+ (Novagen) digested by XhoI and NheI ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were separated by electrophoresis on a 2.5% agarose gel containing GelRed (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Genomics 2020Quote: ... Cultures were routinely tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, USA) to confirm negative infection status.
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bioRxiv - Microbiology 2019Quote: ... The digests were then purified with either GenElute PCR Clean-Up Kit (Sigma-Aldrich) or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination was tested monthly using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). All cells were mycoplasma-free ...
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bioRxiv - Microbiology 2021Quote: ... DNA from tail tips was isolated using Extract-N-Amp Tissue PCR kit (Sigma) and PCR genotyping undertaken as described (The Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with a commercial kit (Sigma JumpStart™ Taq ReadyMix™, P2893) and 1-μL of purified DNA ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed using the SYBR Green JumpStart Taq Ready Mix (Sigma Aldrich) on an ABI Prism 7900 system (Applied Biosystems) ...