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Citations for Millipore Sigma :
501 - 550 of 1005 citations for PCR strip since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned at the NdeI and XhoI restriction sites of pET30a (Novagen) to add a C-terminal 6x-his tag using the Gibson assembly cloning system ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was subsequently cloned into the BamHI-HindIII restriction sites of pETDuet (Novagen) to generate the recombinant plasmid pETDuet-DHS1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The designed promoters were obtained by either PCR (KOD Hot-Start DNA polymerase, Merck-Millipore). PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were purified using GFX PCR DNA and Gel Band Purification Kit (Sigma-Aldrich), according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were tested for mycoplasma using Lookout Mycoplasma PCR kit (Sigma-Aldrich MP0035).
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Cell Biology 2022Quote: ... Regular mycoplasma testing was performed using the LookOut Mycoplasma PCR detection (Sigma, Cat. No. MP0035).
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bioRxiv - Microbiology 2020Quote: ... The 3’UTR was determined by RT-PCR with an oligo-dT anchor primer (Sigma) as described58 with primers found in Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... RT and PCR primers for the determination of the 3’UTR were obtained from Sigma. The remaining primers were purchased from TAG Copenhagen.
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bioRxiv - Developmental Biology 2020Quote: The four lncRNAs tested were PCR-amplified with KOD Xtreme™ DNA Hotstart Polymerase (Millipore) from their 5’ end up until the last codon of the sORF to be tested ...
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bioRxiv - Immunology 2020Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... Positive plants were further confirmed by PCR and western blot using GFP antibodies (Sigma-Aldrich). The positive T2 generation transgenic plants were evaluated in a growth chamber.
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... 2 μL of sample was added to 1x PCR Master Mix (Sigma-Aldrich, Basel, Switzerland) and the 4 degenerate primers at a final concentration of 1 μM each in a final volume of 25 μL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified fragments were purified using spin columns (GenElute TM PCR Clean-Up Kit, Sigma- Aldrich) and were checked on agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2022Quote: ... Clones were screened for N-Se loss by PCR using REDTaq ReadyMix (Sigma, R2523-100RXN) and custom primers (5’-AGCGAGACTCCGTCTAGAAA-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Genomics 2019Quote: ... We performed qRT-PCR using FastStart Universal SYBR Green Master Mix with ROX (Sigma, 4913914001) on a ViiA 7 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich, see Table 2, (Buenrostro et al., 2013) for 5 initial cycles (1 cycle ...
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bioRxiv - Genomics 2019Quote: ... Tail snips were prepared using the REDExtract-N-Amp Tissue PCR Kit (Sigma-Aldrich XNAT) and were screened for Ube3a deletion using PCR primers spanning the expected deletion site ...
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bioRxiv - Genomics 2021Quote: ... Cells tested negative for mycoplasm contamination with the LookOut Mycoplasm PCR Detection Kit (Sigma; MP0035) and Jumpstart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with SacI und SalI and cloned into pET-24a (Novagen), resulting in plasmid pET24a-OLA1-6xHIS ...
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bioRxiv - Microbiology 2019Quote: ... Digested PCR product was ligated into a modified T7 promotor-based pET33b expression vector (Novagen) that contained the super-folding variant of green fluorescent protein encoding gene with C-terminal 6xHis-tag and a modified multiple cloning site ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were selected and screened by PCR using the Expand Long Range dNTPack (Sigma), PCR buffer supplemented with 2% DMSO and the primer pairs for the 5’ region (LR 5’ fw ...
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bioRxiv - Microbiology 2022Quote: ... and were verified to be mycoplasma free by a LookOut Mycoplasma PCR Detection kit (Sigma). C ...
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bioRxiv - Bioengineering 2022Quote: ... Fragments were amplified by PCR using Q5 polymerase and assembled into a pET28a(+) vector (Novagen) via Gibson Assembly using reagents from New England Biolabs (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Captured products were amplified through PCR using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore), cloned into the pET23-P2-D12A-THR expression backbone ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Immunology 2022Quote: ... cDNAs were mixed with indicated gene-specific primers and SYBR green PCR Master Mix (Sigma), and qRT-PCR was performed on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Immunology 2022Quote: ... PCRs with 50 ng of prepared cDNAs were carried out using Jumpstart Taq polymerase (Sigma) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Mice were genotyped by PCR on tail genomic DNA using Red-N extract kit (Sigma), with primers indicated in Supplemental table S3.
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA extraction was performed using Extract-N-Amp(tm) Tissue PCR Kits (Sigma/Aldrich). For cDNA synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloning the PCR product between the NdeI and BamHI sites of pET9a (EMD Millipore). PCR-amplified DNA sequences were confirmed by Sanger sequencing.
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using the KAPA SYBR Fast qPCR Kit (KK4621, Sigma-Aldrich, USA) and universal reverse primer (Table S2) ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR reactions were conducted using KAPA HiFi Hotstart DNA polymerase (Sigma-Aldrich, catalog #BK1000) unless stated otherwise ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into the expression vector pET-30a (Novagen, Madison, WI, USA). The fusion peptide expression and purification were carried out as described previously (Miao et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). In summary ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were validated as mycoplasma free by a PCR-based mycoplasma detection kit (Sigma Aldrich).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the ChIP-PCR assay was performed using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore, 3753379). The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam ...
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bioRxiv - Physiology 2023Quote: ... and PCR was performed using KOD Xtreme™ HotStart DNA Polymerase Kit (71975-3, Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was cloned in the BglII/BamHI sites of pFLAG-CMV2 (Sigma-Aldrich). The A1625G variant was introduced by site-directed mutagenesis using primers ...
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bioRxiv - Developmental Biology 2024Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). All transgenic and KO hESC lines in this study used H9 as the parental line ...
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bioRxiv - Genomics 2023Quote: ... cells were tested for mycoplasma contamination with LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Genetics 2023Quote: ... mm9) amplified by the PCR DIG Probe Synthesis Kit (Cat #11636090910, Sigma-Aldrich, MO, USA). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910 ...
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bioRxiv - Cell Biology 2020Quote: Glomerular DNA was used for PCR using the RedTaq Ready MasterMix (Sigma-Aldrich, St. Louis, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with RED extract-N-Amp Tissue PCR reagents (Millipore Sigma, Saint-Louis, MO) to purify genomic DNA and perform the genotyping PCR ...
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bioRxiv - Biophysics 2022Quote: ... sub-cloned into the pNKS2 vector) via PCR with custom-designed primers (Eurofins Genomics, Sigma-Aldrich) and PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma). SenV Cantell strain was obtained from Charles River Laboratories and used at 200 hemagglutination units/mL (HAU) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...