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Citations for Millipore Sigma :
551 - 600 of 1005 citations for PCR strip since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA was cleaned using a GenElute 96 Well PCR Clean-up kit (Sigma-Aldrich) and sent for sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma) on a CFX Connect Real-Time PCR machine (Biorad) ...
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bioRxiv - Molecular Biology 2019Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich). Infectious stocks of a cell culture-adapted strain of genotype 2A JFH1 HCV were generated and titered in Huh-7.5 cells by focus-forming assay (FFA) ...
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bioRxiv - Molecular Biology 2019Quote: ... H69 cells were Mycoplasma free as established using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and authenticated using STR profiling by ATCC before the start of this study (not shown).
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA the coding sequence for Reb1 was amplified by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were tested every fortnight for Mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich). Cell line identity was confirmed using STR profiling.
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bioRxiv - Physiology 2020Quote: ... Genotypes were confirmed from ear notches by using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) and primers as previously described12 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Systems Biology 2021Quote: ... 15 µL from each PCR product were mixed with 5 µL 6x loading dye solution (Sigma) before being loaded onto a 2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... All cultures were routinely tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection kit (Sigma). To induce differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR analysis was performed using the KAPA SYBR Fast PCR master mix (Sigma Aldrich, #KK4605) or SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA extracted from the whole embryos (using Extract-N-Amp™ Tissue PCR Kit, Sigma) was used for the genotyping after vasculature imaging.
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bioRxiv - Physiology 2022Quote: ... Total genomic DNA from blood was isolated using REDExtract-N-Amp Blood PCR Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Paleontology 2019Quote: 3µl of bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR on both gDNA and cDNA was performed using the SYBR® Green JumpStart (Sigma) in a 7500 Fast Real Time PCR cycler from Applied Biosystems.
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were routinely tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich). Cell lines were authenticated by ATCC with no further authentication carried out after purchase.
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bioRxiv - Neuroscience 2021Quote: ... DNA-free isolation was verified by PCR amplification (RedTaq® Polymerase, catalog no. D4309, Sigma-Aldrich). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... The cell line was tested free of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (SIGMA). Cultured cell monolayers were maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were tested for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma, # MP0035), following the manufacturer’s instructions and using the JumpStart™ Taq DNA Polymerase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... After amplification PCR products were checked on 1% (w/v) agarose gel (Sigma-Aldrich, Darmstadt, Germany) (5 µL of each PCR product mixed with 1 µL 6x loading dye for 50 min at 120 V ...
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bioRxiv - Microbiology 2022Quote: ... Cell lines were tested for mycoplasma using a PCR assay (Venor GeM Mycoplasma Detection Kit, Sigma).
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bioRxiv - Genomics 2023Quote: ... The amplified cDNA was purified using the HighPrep PCR Clean-up System (Sigma Aldrich AC-60050) adding a volume of magnetic beads equal to 0.8x the sample volume ...
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bioRxiv - Synthetic Biology 2023Quote: ... Splicing by overlap extension PCR was performed with the KOD Xtreme Hot Start DNA Polymerase (Novagen). Generally ...
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bioRxiv - Cell Biology 2023Quote: TRPM8 sequences were amplified by PCR using the following primers (ordered from Millipore Sigma, Burlington, MA):
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed in 25 µl with JumpStart RED Accutaq LA polymerase (Sigma-Aldrich, cat# D8045) in the supplied buffer ...
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bioRxiv - Immunology 2023Quote: ... Cells were continuously verified as mycoplasma free using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... All cell lines were confirmed mycoplasma free using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Plant Biology 2024Quote: ... PCR-grade H2O and RedTaq Ready Mix according to the manufacturer’s instructions (Sigma-Aldrich, Rehovot, Israel). The amplified products were subjected to electrophoresis on 1.2% agarose gel containing agarose in 0.5 X TBE buffer and were stained with ethidium bromide ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture consisted of Kappa Probe Fast qPCR Master Mix (2X) Kit (Sigma-Aldrich), 0.75 μL of primers and 2.5 μL of RNA in a final volume of 10 μL reaction ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR products were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). Cleaned PCR2 products were quantified using Qubit® dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) and DBNL(1-374) were amplified by PCR and cloned into pET28a vector (Novagen) between BamHI and NotI sites to obtain constructs for expression of his-tagged proteins in E ...
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bioRxiv - Microbiology 2020Quote: We performed reverse transcription using the First strand cDNA synthesis kit for RT-PCR (AMV) (Sigma-Aldrich) and the protocol described by the supplier ...
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bioRxiv - Genetics 2021Quote: ... cells tested for several strains of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed using gene-specific primers (Table S3) with Kapa SyBr Fast (Sigma-Aldrich, #KK4618) in an Applied Biosystem ViiA 7 real-time PCR system.
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bioRxiv - Microbiology 2019Quote: ... the plasmid containing the insert was linearized and purified with Gene-Elute PCR Clean-Up Kit (Sigma). DNA concentration was measured with Qubit (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative PCR mixtures (10 μL) contained 5 μL Kapa SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich), 300 nM of the primer pair ...