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Citations for Millipore Sigma :
551 - 600 of 1048 citations for PCR strip since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was cloned in the BglII/BamHI sites of pFLAG-CMV2 (Sigma-Aldrich). The A1625G variant was introduced by site-directed mutagenesis using primers ...
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bioRxiv - Genetics 2023Quote: ... mm9) amplified by the PCR DIG Probe Synthesis Kit (Cat #11636090910, Sigma-Aldrich, MO, USA). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910 ...
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bioRxiv - Developmental Biology 2024Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). All transgenic and KO hESC lines in this study used H9 as the parental line ...
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bioRxiv - Genomics 2023Quote: ... cells were tested for mycoplasma contamination with LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Developmental Biology 2022Quote: ... Mice were genotyped by PCR on tail genomic DNA using Red-N extract kit (Sigma), with primers indicated in Supplemental table S3.
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA extraction was performed using Extract-N-Amp(tm) Tissue PCR Kits (Sigma/Aldrich). For cDNA synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloning the PCR product between the NdeI and BamHI sites of pET9a (EMD Millipore). PCR-amplified DNA sequences were confirmed by Sanger sequencing.
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using the KAPA SYBR Fast qPCR Kit (KK4621, Sigma-Aldrich, USA) and universal reverse primer (Table S2) ...
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bioRxiv - Developmental Biology 2023Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). In summary ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were validated as mycoplasma free by a PCR-based mycoplasma detection kit (Sigma Aldrich).
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bioRxiv - Genetics 2024Quote: ... The PCR product was cloned into the expression vector pET-30a (Novagen, Madison, WI, USA). The fusion peptide expression and purification were carried out as described previously (Miao et al. ...
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bioRxiv - Microbiology 2024Quote: ... All strains were verified to be Mycoplasma-negative using LookOut mycoplasma PCR detection kit (Sigma). For chlamydial transformations ...
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bioRxiv - Cell Biology 2024Quote: ... The ChIP-PCR assay was performed using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore, 3753379) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... All PCR reactions were conducted using KAPA HiFi Hotstart DNA polymerase (Sigma-Aldrich, catalog #BK1000) unless stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: Glomerular DNA was used for PCR using the RedTaq Ready MasterMix (Sigma-Aldrich, St. Louis, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were treated with RED extract-N-Amp Tissue PCR reagents (Millipore Sigma, Saint-Louis, MO) to purify genomic DNA and perform the genotyping PCR ...
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bioRxiv - Biophysics 2022Quote: ... sub-cloned into the pNKS2 vector) via PCR with custom-designed primers (Eurofins Genomics, Sigma-Aldrich) and PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma). SenV Cantell strain was obtained from Charles River Laboratories and used at 200 hemagglutination units/mL (HAU) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA was cleaned using a GenElute 96 Well PCR Clean-up kit (Sigma-Aldrich) and sent for sequencing.
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bioRxiv - Developmental Biology 2019Quote: ... and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma) on a CFX Connect Real-Time PCR machine (Biorad) ...
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bioRxiv - Molecular Biology 2019Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich). Infectious stocks of a cell culture-adapted strain of genotype 2A JFH1 HCV were generated and titered in Huh-7.5 cells by focus-forming assay (FFA) ...
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bioRxiv - Molecular Biology 2019Quote: ... H69 cells were Mycoplasma free as established using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and authenticated using STR profiling by ATCC before the start of this study (not shown).
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA the coding sequence for Reb1 was amplified by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were tested every fortnight for Mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich). Cell line identity was confirmed using STR profiling.
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bioRxiv - Physiology 2020Quote: ... Genotypes were confirmed from ear notches by using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) and primers as previously described12 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Systems Biology 2021Quote: ... 15 µL from each PCR product were mixed with 5 µL 6x loading dye solution (Sigma) before being loaded onto a 2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... All cultures were routinely tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection kit (Sigma). To induce differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR analysis was performed using the KAPA SYBR Fast PCR master mix (Sigma Aldrich, #KK4605) or SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA extracted from the whole embryos (using Extract-N-Amp™ Tissue PCR Kit, Sigma) was used for the genotyping after vasculature imaging.
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bioRxiv - Physiology 2022Quote: ... Total genomic DNA from blood was isolated using REDExtract-N-Amp Blood PCR Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Paleontology 2019Quote: 3µl of bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR on both gDNA and cDNA was performed using the SYBR® Green JumpStart (Sigma) in a 7500 Fast Real Time PCR cycler from Applied Biosystems.
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were routinely tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich). Cell lines were authenticated by ATCC with no further authentication carried out after purchase.
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bioRxiv - Neuroscience 2021Quote: ... DNA-free isolation was verified by PCR amplification (RedTaq® Polymerase, catalog no. D4309, Sigma-Aldrich). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... The cell line was tested free of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (SIGMA). Cultured cell monolayers were maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were tested for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma, # MP0035), following the manufacturer’s instructions and using the JumpStart™ Taq DNA Polymerase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... After amplification PCR products were checked on 1% (w/v) agarose gel (Sigma-Aldrich, Darmstadt, Germany) (5 µL of each PCR product mixed with 1 µL 6x loading dye for 50 min at 120 V ...