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Citations for Millipore Sigma :
351 - 400 of 1005 citations for PCR strip since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Amplified S-gene and polymerase chain reaction (PCR) engineered pET31b(+) (Novagen, Germany) bacterial expression vector were amplified using 0570F and 0571R primers ...
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bioRxiv - Biochemistry 2022Quote: ... brucei genomic DNA using PCR and ligated into the pET15b vector (Novagen), which provides an N-terminal His6 tag followed by a thrombin cleavage site prior to the target protein ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR was performed using KOD Hot start DNA polymerase (Millipore Sigma) since it has a low error rate unlike Taq polymerase (Engler and Marillonnet 2013) ...
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bioRxiv - Physiology 2022Quote: ... and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020). 300 ng of the eluted DNA was digested overnight at 37°C with the restriction enzymes including USP8 – BstBI and USP48 – SpeI ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was performed using JumpStart REDTaq Ready Mix (Sigma, Cat#P0982-800RXN) and the primers listed below ...
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bioRxiv - Molecular Biology 2022Quote: ... and splice isoform specific PCRs were performed (Sigma Aldrich High fidelity Taq) using appropriate primers (Supplemental table 8 ...
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bioRxiv - Systems Biology 2022Quote: ... PCR was performed with a proof-reading KOD polymerase (Sigma-Aldrich, 71086) using vector-specific primers.
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bioRxiv - Neuroscience 2023Quote: ... and PCR amplification was performed using KAPA2G ReadyMix Kit (Sigma Aldrich, KK5103) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCRs were performed using KOD Hot Start DNA polymerase (EMD Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: PCRs were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) using the following conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification was performed using KOD Hot Start Master Mix (Millipore Sigma) with primers listed in Supplementary Table 6 ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using the KOD Hotstart DNA polymerase (Merck Millipore). Plasmid isolation ...
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bioRxiv - Neuroscience 2023Quote: ... Following the RED Extract-N-Amp Tissue PCR XNAT manufacturer’s protocol (Sigma), 2-3mm of each mouse’s tail was removed and DNA was extracted ...
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bioRxiv - Microbiology 2023Quote: ... The PCR mixture consisted of MTP Taq DNA Polymerase (Sigma-Aldrich, Germany) (0.05 u/μL ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed using KOD Hot Start DNA Polymerase (EMD Millipore). In the via-1 strain ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction contained 1.25 U JumpStartTM Taq DNA Polymerase (Sigma-Aldrich), 1× PCR Buffer (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sanger sequencing of the PCR products was performed using commercial service (Sigma). The sequencing traces were examined and the fish carrying A to G mutation were selected ...
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bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted cDNA was amplified by PCR using the Sybr Green Master Mix (Sigma), and raw threshold-cycle time (Ct ...
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bioRxiv - Molecular Biology 2021Quote: ... the TADA2b cDNA fragment was PCR amplified and cloned in pET15b vector (Novagen) using Nde I and Bam HI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma) and primers P3 and P4 ...
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bioRxiv - Molecular Biology 2019Quote: ... but tested negative for mycoplasma by PCR-based detection kit (Sigma-Aldrich D9307). Medium containing 0.5mM palmitate was prepared by first dissolving FFA-free BSA (Fisher Scientific BP9704100 ...
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bioRxiv - Biophysics 2019Quote: ... We visualized PCR products and DNA plasmids by 1 % agarose (SIGMA Cat.#A9539) gel dyed with GelRed (BIOTIUM Cat.#41003) ...
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bioRxiv - Pathology 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035). iPSC lines for the iMg were cultured by the Human Pluripotent Stem Cell Core (CHOP).
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bioRxiv - Immunology 2019Quote: ... Respective cDNA regions were PCR amplified and cloned into the pET24b vector (Novagen) for bacterial expression ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the gene encoding CAT was PCR-amplified from pACYC-Duet™-1 (Novagen) to include a 3’ flexible linker (GTSAAAG ...
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bioRxiv - Immunology 2019Quote: ... Cells were verified as mycoplasma-free using the LookOut PCR detection Kit (Sigma).
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bioRxiv - Bioengineering 2019Quote: PCR master mix: 1x KOD Xtreme Hot Start DNA Polymerase buffer (EMD Millipore), 1.2 µM (each ...
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bioRxiv - Genomics 2020Quote: ... a specific FAM digital PCR assay was designed (Sigma-Aldrich, Gillingham, Dorset, UK) targeting the promotor region of RASSF1A and five 5’-CGCG-3’ BstUI recognition sequences ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product designed for homologous recombination was transformed using lithium acetate (Sigma) as previously described (Tripp et al. ...
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bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
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bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Inverse PCR using this construct was carried out with KOD polymerase (EMD Millipore) according to manufacturers instructions ...
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bioRxiv - Microbiology 2021Quote: PCR reactions were carried out with either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNA was purified with a GenElute PCR cleanup kit (Sigma-Aldrich), and concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Conventional PCR was performed using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was subsequently checked for DNA by PCR using RedTaq readymix (Sigma). RNA integrity was assessed using 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... mm9) ampli?ed by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, 11636090910). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Nectin-3 alpha was PCR amplified (KOD hot start DNA polymerase, Sigma-Aldrich) from a mouse brain cDNA library using the forward primer ...
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bioRxiv - Genetics 2019Quote: ... PCR plates were filled with a master mix for KOD Hotstart (Novagen #71086) PCR reactions containing Lister427 gDNA according to manufacturer’s specifications and primer pairs specific to each ORF were transferred into the associated well number for each plate by TECAN Freedom EVO 150 pipetting instrument ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR product was digested with NcoI and XhoI and cloned into pET28a (Novagen) in frame with a C-terminal his-tag coding sequence E ...
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bioRxiv - Cell Biology 2019Quote: ... PCR was performed using the KOD Hot Start DNA polymerase kit (Novagen, TOYOBO). The list of primers used for RT PCR is indicated on table 3.
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... Mycoplasma was not detected with the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
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Orange is the new white: taxonomic revision of Antarctic Tritonia species (Gastropoda: Nudibranchia)bioRxiv - Zoology 2020Quote: ... 3.3 μL REDExtract-N-Amp PCR ReadyMix (Sigma Aldrich, St. Louis, MO, USA), 0.3 μL of each primer ...
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bioRxiv - Molecular Biology 2021Quote: ... bL33 were PCR-amplified and cloned individually into the pET24(a) plasmid (Novagen). Similarly ...