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Citations for Millipore Sigma :
4201 - 4250 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Heart lysates were first pre-cleared with 20 μl of packed protein A beads (Sigma) per lysate on a rocker or tumbler at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Physiology 2022Quote: ... Oocytes expressing desired proteins were harvested in ice-cold CelLytic M lysis buffer (Sigma-Aldrich) supplemented with proteinase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were eluted in HBS containing 1 mM DTT and 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Neuroscience 2021Quote: ... 100 μg of total protein for each group were reduced with 5 mM dithiothreitol (Sigma) for 30 min at 56 °C and then alkylated with 11 mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... protein-A sepharose and ANTI-FLAG® M2 Affinity Gel beads (Sigma Aldrich, Cat# A2220) were washed three times with 1x PBS and two times with IP100 buffer (25 mM Tris-HCl 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to a 0.45 µm PVDF membrane (EMD Millipore, Billerica, MA, USA), and the blot was probed with anti-AGO2 mouse monoclonal antibody (Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins by SDS-PAGE were transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore), washed with TBST for 5 minutes and then were blocked with 5% BSA in TBST[0.1%(v/v ...
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bioRxiv - Molecular Biology 2021Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 4°C for 1 hour with rocking ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto a polyvinylidene fluoride membrane (Immobilon R, Merck Millipore Ltd) for 1 hour at 20 V ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was transferred to a new tube and protein concentration determined by Direct Detect (Millipore). 1mg of lysate per immunoprecipitation was used for the following steps ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Protein degradation was triggered by addition of 0.5 mM indole-acetic acid (IAA; Sigma-Aldrich) to the growth medium.
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bioRxiv - Biophysics 2019Quote: ... the protein sample was filtered using a 30 kDa centricon filter (Merck Millipore, Darmstadt, Germany). The crude protein was then injected into a DEAE anion exchange column equilibrated with 10 mM Tris (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...
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bioRxiv - Microbiology 2019Quote: ... was achieved by incubating protein samples with 1 unit/ml of restriction grade thrombin (Novagen). This was carried out overnight at 4°C in 25 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then incubated with protein A magnetic beads (Merc Millipore Cat. No. LSKMAGA10) for 1h at +4 °C (with rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoblots of targeted proteins were visualized using HRP-conjugated secondary antibodies (Catalog No. AP187P, Millipore) with SuperSignal™ West Femto Maximum Sensitivity Substrate (Catalog No ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane and probed with anti-HA-HRP (3F10) (Sigma), mouse anti-GFP (ab6556 ...
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bioRxiv - Cell Biology 2019Quote: ... Pure protein was concentrated using a 30 KDa molecular-weight cutoff ultracentrifugal concentration device (Millipore).
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bioRxiv - Plant Biology 2020Quote: ... Proteins samples were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore) for 16 h at 100 mA ...
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bioRxiv - Physiology 2020Quote: ... The protein precipitate was collected on a GF/C glass microfiber filter (Whatman, Sigma Aldrich) and then mixed with EcoScint (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: Frozen cortexes were homogenized in radioimmunoprecipitation assay (RIPA) buffer containing a protein inhibitor cocktail (Sigma). Samples were centrifuged at 7000 g for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The proteins were detected with the EMD Millipore Luminata Western HRP chemiluminescence substrate (WBLUF0500; Millipore) and the signals were visualized with the Western blotting system (C600 ...
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bioRxiv - Biophysics 2019Quote: ... The dissolved protein was concentrated in 3 kDa centrifuge units (Millipore Sigma, St. Louis, MO) to 1 mg/mL (αS ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were resolved by 10% SDS-PAGE and electrotransferred onto polyvinylidene fluoride membrane (PVDF) (Millipore) in transfer buffer (10 mM CAPS ...
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bioRxiv - Microbiology 2019Quote: ... Protein expression was induced by addition of 0.5 mM of isopropyl β-d-thiogalactopyranoside (Sigma) at 25 °C for 24 h before being harvested ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Genomics 2020Quote: ... Protein was precipitated from each fraction with 1/10th volume trichloroacetic acid (TCA, Sigma-Aldrich), incubated overnight at −20°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were concentrated in an Amicon Ultra concentrator tube (100 kDa molecular weight cutoff, Millipore). Final protein concentration was quantified based on tryptophan fluorescence measured at 340 nm on a fluorometer (Hitachi).
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Molecular Biology 2019Quote: ... Thrombin was removed from protein-containing fractions with 40 μL p-aminobenzamidine beads (Sigma #A7155) washed twice with 400 uL 137 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... protein production was induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma #I6758), and the culture was incubated at 37°C and 250 rpm for 4 hr ...
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bioRxiv - Microbiology 2019Quote: ... Protein A/G Plus agarose beads were blocked with bovine t-RNA (1mg/mL; Sigma) for 2h in modified-RIPA buffer ...
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bioRxiv - Genetics 2020Quote: ... Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663). After reverse crosslinking ...
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bioRxiv - Microbiology 2019Quote: WT ppr-SiiA protein and mutant variants were produced using freshly transformed BL21(DE3) (Novagen) cells ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific protein binding sites were blocked with 4% normal donkey serum (NDS; Sigma-Aldrich, D9663) with 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: ... The protein pellet was resuspended in 100µl of 50mM Triethylammonium bicarbonate (TEAB, Sigma-Aldrich, USA). Trypsin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The protein pellet was resuspended in 100µl of 50mM Triethylammonium bicarbonate (TEAB, Sigma-Aldrich, USA). Trypsin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Bioengineering 2019Quote: Proteins were isolated from cells cultured on films or scaffolds in RIPA buffer (Sigma-Aldrich), supplemented with cOmplete™ ...
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein was then transferred from the gel to a PVDF membrane (Immobilon-P, Millipore) in transfer buffer (20% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... and then the proteins were immunoprecipitated with anti-FLAG M2 affinity gel (Sigma-Aldrich, M8823), anti-GFP mAb-magnetic agarose (MBL ...
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bioRxiv - Microbiology 2021Quote: Vero cells were transfected with spike protein expression constructs using the GeneJuice transfection reagent (Novagen). The indicated S-protein expression constructs (1 µg ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular and histone proteins were precipitated by incubating samples with 6M ammonium acetate (Sigma; A1542) on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Viral supernatants were filtered through a 0.45 μm (low protein binding) filter unit (EMD Millipore). The filtered retroviral supernatant was then concentrated 20-fold using an Amicon Ultra-15 concentrator (EMD Millipore ...