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Citations for Millipore Sigma :
4301 - 4350 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... were dissected from E13.5 wild-type mice and plated in a 8-wells chamber coated with 10µg/ml poly-d-lysin (Sigma-Aldrich, St Louis, MO) and 10µg/ml mouse Laminin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Immunology 2020Quote: ... the synthesized mRNA was isolated from the reaction mix by precipitation with half volume of 8 M LiCl solution (Sigma-Aldrich, Hamburg, Germany), and finally the pellet was dissolved in nuclease-free water ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Balb/C mice at 8 weeks of age received a single iv injection of ADR (10.5 mg/kg, Sigma Aldrich, St. Louis, MO) via tail vein injection and were followed for a total of 6 weeks (48) ...
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bioRxiv - Immunology 2021Quote: Samples obtained from in vitro proteolysis assay were analyzed by electrophoresis on an 8% SDS-polyacrylamide gel followed by transfer to Immobilon polyvinylidene difluoride membranes (Millipore Co., Bedford, MA). The membranes were blocked using 4% non-fat milk and immunostained with specific mAb ...
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bioRxiv - Cell Biology 2022Quote: ... Viruses were added to cells in a 1:1 ratio with DMEM supplemented with 10% FBS with 8 μg/mL polybrene (H9268-5G; Millipore Sigma, Darmstadt, Germany). For MRC-5 ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with the wash buffer (20mM Tris pH 8, 300 mM NaCl, 20mM imidazole [Sigma-Aldrich; I5513], 1 mM DTT) three times and eluted with the elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Worms were cultured in 6 well dishes (4-5 mature male worms or 8-10 immature worms in 3mL media per well) at varying concentrations of praziquantel (PZQ, Sigma-Aldrich cat. # P4668) or DMSO vehicle control and imaged to record phenotypes.
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2020Quote: ... sections were treated with Weigert’s Iron Hematoxylin for 8 min to stain nuclei and Direct Red 80 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 1 hour at room temperature to visualize collagen before washing with 0.5% glacial acid ...
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bioRxiv - Neuroscience 2019Quote: ... Pregnant Wistar rats (293.0–428.7 g) therefore received a single intraperitoneal (i.p.) injection of either poly (I:C) (n=8; P9582, potassium salt, Sigma-Aldrich; Gillingham, Dorset, United Kingdom) at a dose of 10 mg/kg or 0.9% non-pyrogenic sterile saline as a control (n=8 ...
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bioRxiv - Physiology 2020Quote: ... 8-12 week old mice (males and females) were injected IP with D-gal (Sigma Aldrich, Cat# G0500, 350 mg/kg body weight) and LPS (ENZO life sciences ...
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bioRxiv - Cell Biology 2019Quote: ... The filter was then washed with 8 ml of 1% BSA PBS (diluted from SRE0036 SIGMA in CaCl/MgCl free HPBS + Rnase Inh) and nuclei suspension spun in a precooled (4°C ...
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bioRxiv - Cell Biology 2020Quote: Confluent primary mouse keratinocytes in 10-cm petri dishes were washed twice with ice-cold PBS followed by incubation with 8 ml of Biotin-NHS (Sigma, cat. no. 203112) in PBS (0.25 mg/ml ...
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bioRxiv - Physiology 2020Quote: Bone marrow from Winnie and control mice tibiae (n=4-8 mice/group) was collected by flushing with α-modified essential medium ((α-V1EV1, Sigma, Australia). Bone marrow-derived mesenchymal stromal cells (BM-MSCs ...
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bioRxiv - Cancer Biology 2021Quote: The migration of two TNBC cells (MB231, MB468) were performed using a 24-well transwell chambers (8 µm pore size; Millipore, Billerica, MA, USA). The cells were plated in triplicates into the upper chamber (1 × 105 cells/well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Cell Biology 2020Quote: ... 3T3-L1s were changed to serum-free DMEM supplemented with 0.2% BSA FFA-free (Sigma-Aldrich, St. Louis, USA, Catalog 9048-46-8). The media was supplemented with 1% DMSO for negative control or 1uM isoproterenol to induce lipolysis +/- 100 μM Atglistatin (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... were performed prior to incubation for 8 h with biotinylated mouse monoclonal antibody 4G8 (2 μg/ml, Sigma-Aldrich SIG-39240 Beta-Amyloid). Staining was completed with the Ventana XT DABMap kit and a haematoxylin counterstain ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
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bioRxiv - Bioengineering 2020Quote: Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were separated on 8% SDS-PAGE gels and transferred to a PVDF membrane (immobilon FL; Sigma-Aldrich, St Louis, MO, USA). Membranes were blocked with 1% (for TMEM16A protein ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The beads were then removed by magnetic separation and cells were spinoculated with HIV-GFP-Thy1.2 (pNL4-3-−Δ6-dreGFP-CD90) and 8 ug/mL polybrene (Millipore Sigma; TR-1003-G) for 2 hrs at 1100 x g ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated DA neurons were perfused with external solution containing CPA for more than 8 min and then additionally exposed to 20 mM caffeine (Sigma-Aldrich, USA, C0750) to confirm the complete depletion of ER Ca2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Hydrogel was prepared separately as solution I and II: Solution I was prepared by dissolving 8 g (16% (wt/vol)) paraformaldehyde (Sigma-Aldrich, Steinheim, Germany) (PFA ...
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bioRxiv - Plant Biology 2022Quote: ... Each RNA sample was extracted from 800 μl (8 IP-Star tubes) of RNA IP product using 1 ml of TriReagent (Sigma-Aldrich T9424, USA) as indicated by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... were dosed 2 mL/Kg intraperitoneally (IP) twice weekly with either olive oil (N=8) or CCl4 (CCl4:olive oil, 1:3, [v:v], Sigma-Aldrich, St Louis, MO) for 4 weeks ...
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bioRxiv - Plant Biology 2023Quote: ... ABA treatments were initiated by transferring 8-day-old seedlings in mass to plates containing 50 µM ABA (Sigma cis/trans-abscisic acid) or to plates containing vehicle control (Ethanol) ...
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bioRxiv - Neuroscience 2024Quote: ... pups PND 8-9 were given an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 40 μg/kg; E. coli O111:B4, Sigma; dissolved in sterile saline) or vehicle (saline) ...
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bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Biophysics 2024Quote: ... Embryos together with 4 μL of 0.75× Egg Salt buffer were layered on top of 8 μL of 0.75× Egg Salt buffer with 75% Percoll (vol/vol; Sigma, St. Louis, MO, USA), and spun in the CPM ...
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bioRxiv - Cell Biology 2023Quote: U2Os-AP2-GFP cells were transduced with lentiviral media expressing respective shRNAs in the presence of Polybren 8 μg/ml (Sigma-Aldrich, TR-1003) for 5 hours ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by transduction with LV at MOI 3 in the presence of 8 µg/ml Hexadimethrine bromide (Sigma-Aldrich, St. Louis, MO, USA) the following day ...
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bioRxiv - Cell Biology 2023Quote: ... and after removing these contaminating cells the final cell suspension was plated into wells of an 8-well cell culture slide (Millicell® EZ Slides, Merck Millipore, Darmstadt, Germany) coated with human fibronectin (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed in PBS and placed in a humid chamber to be stained with the primary antibody anti-HA tag at a concentration of 8 μg/mL (anti-rabbit, #H6908, Sigma-Aldrich, Deisenhofen, Germany) in PBS ...
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bioRxiv - Immunology 2023Quote: ... Samples were lysed with modified RIPA buffer (50 mM Tris, 150 mM NaCl, 0.1% SDS, 2% Octyl glucoside (Sigma-Aldrich, 29836-26-8), 0.5% sodium deoxycholate and 1% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Systems Biology 2024Quote: hCMEC/D3 cells were cultured on Lab-Tek™ Permanox™ 8-well chamber slides coated with calf skin collagen (Sigma-Aldrich, UK), prior to immunostaining according to standard protocols74 and using a primary antibody directed against zona occludens-1 (ZO-1 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dissociated using StemPro Accutase and reseeded in 8 well µ-Slides (IBDI #80826) pre-coated with 15 µg/mL poly-L-ornithine (Sigma #P-3655), 1 µg/mL laminin (Sigma #L4544) ...
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bioRxiv - Cancer Biology 2024Quote: ... For infection cells were plated in 60- or 100-mm tissue culture dishes and allowed to achieve 60% confluence before adding viral supernatant in the presence of 8 μg/ml polybrene for 24 hours (Sigma, St. Louis, MO). Cells were selected by treatment with puromycin (1ug/ml for FaDu cells and 2ug/ml for HaCaT ...
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bioRxiv - Neuroscience 2024Quote: Archived formalin-fixed coronal brain slabs (stored for up to 8 years) were rinsed for 24 hours in PBS and then incubated overnight in fresh neutral buffered 10% formalin (Sigma Aldrich, catalog # HT501128). Two slabs from each donor were selected for imaging corresponding to slices nearest the frontal and occipital horn of the lateral ventricles ...
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bioRxiv - Molecular Biology 2024Quote: ... 30-60 µg protein extracts were fractionated using 8-10% SDS-PAGE under reducing conditions and transferred onto Immobilon-FL PVDF (Millipore, cat no# IPFL00010) membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...