Labshake search
Citations for Millipore Sigma :
4251 - 4300 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... 1.8 µg/cm2) coated hanging culture inserts (Millicell Hanging Cell Culture Insert, polyethylene terephthalate, 1.0 µm pore size, EMD Millipore, MA, USA) and maintained at 37°C in 5 % CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
-
bioRxiv - Genomics 2023Quote: ... then transferred to a microcentrifuge tube on ice which had previously been coated with bovine serum albumin (BSA) by washing with 0.5 mL of 10% (v./v.) BSA (Sigma 9048-46-8) in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... the desired number of embryos were aggregated at the uncompacted 8-cell stage and cultured together in a single microdroplet of global® medium under mineral oil (Sigma, M8410). Embryo aggregation was encouraged by placing them in contact with each other in micro-indented wells in 3.5mm petri dishes (Falcon ...
-
bioRxiv - Biophysics 2023Quote: ... the master SU-8 2050 photoresist was treated with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich, Saint Louis, MO) in a desiccator for overnight ...
-
bioRxiv - Microbiology 2023Quote: ... Transductions were performed via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL protamine sulfate (Millipore Sigma #P3369). Cells were nucleofected with SLC35A2 RNPs two days later ...
-
bioRxiv - Plant Biology 2023Quote: ... cerevisiae was isolated from liquid YPD cultures 8 hours after inoculation from single colonies using the formamide-EDTA method (59) followed by RNAzol® RT (Sigma-Aldrich) extraction ...
-
bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
-
bioRxiv - Immunology 2023Quote: ... viral transduction was performed by spin-infection with 2,000 g at 33 °C for 2 hours in the presence of 8 μg/ml polybrene (Sigma-Aldrich, cat# H9268), followed by incubation for another 4 hours ...
-
bioRxiv - Developmental Biology 2023Quote: ... 210,000 cells each of asynchronous and mitotic cells were seeded (same density as 60,000 cells per well of the 8 chambered cover glass) onto a 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1mg/mL) coated in PBS (1 hour at 37℃ ...
-
bioRxiv - Biochemistry 2023Quote: ... the glycopeptides were heat treated to inactivate the trypsin and the sample was subject to treatment with PNGase F (250U, Sigma; pH 8) followed by PNGase A (NEB ...
-
bioRxiv - Cancer Biology 2023Quote: Whole cell protein lysates were obtained by lysing cell pellets on ice in NP-40 buffer (150mM NaCl, 1% NP-40, 50mM tris pH 8) with 10x Protease Inhibitor Cocktail (Sigma-Aldrich, P8340). Protein quantification was done using the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
-
ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
-
bioRxiv - Systems Biology 2024Quote: ... transferred onto nitrocellulose membrane (Amersham #10600041) in transfer buffer (8 mM Tris, 30 mM Glycine, 20 % Methanol) and stained with Ponceau S (Sigma-Aldrich, #P7170). Membranes were blocked in TBS-T + 5% BSA for 1 hr at RT and incubated overnight at 4°C in primary antibodies prepared in TBS-T + 5% BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... drugs were diluted in AHL saline to the following final concentrations: 100 µM Picrotoxin (P1675-1G, Sigma-Aldrich, CAS 124-87-8), 50 µM CGP54626 hydrochloride (1088/10 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell infection was performed by adding lentiviral particles to cell lines in the presence of 8 μg/ml polybrene (Sigma, TR-1003) for 12 h followed by fresh media addition ...
-
bioRxiv - Immunology 2024Quote: ... Concentrated virus particles solubilized in IMDM were applied to HL60 cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich #H9268). Clones expressing sgRNAs were selected with 2 μg/ml puromycin ...
-
bioRxiv - Microbiology 2024Quote: ... Then 8 µL samples were spotted on a silicon disk coated with 0.01% (w/v) poly-L-lysine (Merck Millipore, Billerica, MA, USA). Cells were fixed with 2.5% (v/v ...
-
bioRxiv - Molecular Biology 2024Quote: ... then stored in sterilized polypropylene bottles (Nalgon, Cat. No. 2330) at 8° C until filtered using 0.22 µm Sterivex filters (Merck-Millipore, Cat. no. SVGP01050) in 60 mL increments using disposable syringes ...
-
bioRxiv - Microbiology 2024Quote: ... lungs were carefully removed from the hydrogel and rinsed in 0.1M PBS and transferred to clearing solution (8% sodium dodecyl sulfate, SDS, Sigma-Aldrich Inc, USA) in 0.1M PBS containing 50mM sodium sulfite (Sigma-Aldrich Inc ...
-
bioRxiv - Cell Biology 2024Quote: ... E25PV4550S) and added to HeLa-TetON-WTOTC cells (65) cultured in a media supplemented with 8 μg/mL polybrene (Sigma Aldrich, H9268). 48 hours later ...
-
bioRxiv - Immunology 2024Quote: ... Sub-confluent A549 cells were subsequently transduced with 2 mL of the filtered lentivirus-containing supernatant supplemented with 8 µg/mL polybrene (Sigma-Aldrich; 107689) for 24h ...
-
bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G). Transduced cells were selected by either puromycin (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
-
bioRxiv - Genomics 2020Quote: ... were stimulated for 48 hours with PMA+ionomycin (phorbolmyristate acetate 25 ng/ml, Fisher Scientific, Hanover, IL; ionomycin calcium salt, 1 μg/ml, Sigma-Aldrich, St. Louis, MO) and treated with hydrocortisone (28 nmol/l ...
-
bioRxiv - Microbiology 2021Quote: ... the protein was quantified by PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Rockford, IL, USA) and the carbohydrate was quantified by Total Carbohydrate Assay Kit (Sigma-Aldrich, St. Louis, MO, USA).
-
bioRxiv - Neuroscience 2023Quote: ... embedded sections were immunohistochemically stained with a biotinylated pan-Aβ antibody Ab5 120 (1:500) or anti-FLAG antibody (Sigma-Aldrich Inc, St. Louis, IL) and developed using Vectastain Elite ABC Kit (Vector Laboratories ...
-
bioRxiv - Developmental Biology 2020Quote: ... The minced kidneys were digested for 8 minutes at 37C in 2mL of 0.25% w/v Collagenase A/1% w/v Pancreatin (Sigma-Aldrich; Cat. 101378001, P1750), with manual dissociation via pipetting through a P1000 tip every 2 minutes ...
-
bioRxiv - Systems Biology 2022Quote: ... medium was exchanged for 450 μl per well of fresh culture medium containing 8 μg/ml polybrene (Millipore cat. no. TR-1003-G), which was also used to dilute the viral stock ...
-
bioRxiv - Immunology 2022Quote: Bone marrow cells isolated from femur and tibia of C57BL/6J mice were differentiated into BMDMs for 7 – 8 days by a standard procedure (65) in DMEM (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Head extractions were incubated at increasing concentrations of the water-soluble standard cardenolide ouabain (10−8 M to 2 × 10−3 M, Sigma, Germany, O3125-1G) at 37°C under the following conditions ...
-
bioRxiv - Cancer Biology 2019Quote: ... For tumor induction 8-week old mice and Cre negative Ptchf/f animals were orally administered three times with 1 mg TAM (Sigma, St Louis, MO) dissolved in 10% ethanol/corn oil ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were placed between two coverslips and STORM buffer (50 mM Tris pH 8, 10 mM NaCl, 10% glucose, 50 mM cysteamine (Sigma, 60-23-1), 0.5 mg/ml glucose oxydase (Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: WT and DCLK1+ cells were plated at 3 x 10^5 cells/well into 8-well Millicell EZ chamber slides (Millipore, cat. no. PEZGS0816) overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
-
bioRxiv - Biochemistry 2021Quote: ... rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM; Sigma-Aldrich, St. Louis, MO), Atto 465-p (8 μM) ...
-
bioRxiv - Biophysics 2021Quote: ... Cat #TIB-180) were subjected to UV-crosslinking and U2AF2–RNA complexes were immunoprecipitated with 8 μg anti-U2AF2 antibody (Sigma-Aldrich, Cat #U4758) and Dynabeads Protein G (ThermoFisher Sci. ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.8 M guanidinium chloride, 8 U/ml proteinase K, 0.5% Triton X-100, pH 8.0; all chemicals are from Sigma-Aldrich, St. Louis, MO, USA), and incubated ON at 50°C in a humified chamber.
-
bioRxiv - Neuroscience 2020Quote: ... and the retina peeled off the pigment epithelium and sclera and placed receptor-side-up onto a piece of filter paper (13 mm diameter, 8 μm pore size, Millipore, Amsterdam, The Netherlands). Vacuum was applied to the other side of the filter in order to suck away the vitreous and attach the isolated retina firmly to the paper ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were infected with APOBEC3A wild-type or catalytically inactive mutant APOBEC3AE72A viral particles for 24 hours at 37°C with 8 μg/mL of polybrene (Millipore #TR-1003-G). PC9 cells were selected in 600 μg /mL of G418 (Gibco #10131035 ...
-
bioRxiv - Physiology 2021Quote: Group of female B6 mice (8 wk old) was injected in oestrus stage with eCG (G4877, 5IU, Sigma Aldrich, Saint Louis, Missouri, USA) followed after 48 h by hCG (Chorulon ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were collected in serial wells of a 24 well plate in 1x PBS with 0.02% sodium azide (NaN3; Sigma-Aldrich – 26628-22-8) and stored at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: ... Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Brain homogenate was digested on a rotating platform at 37° C for 30 minutes in HBSS with 28 U/ml DNase1 and 8 U/ml papain (Sigma, St. Louis, MO). The samples were triturated after 15 and 30 minutes digestion ...
-
bioRxiv - Molecular Biology 2021Quote: ... Melan-ink4a cells were plated at 8.5 × 104 cells/well in 12-well plates and transduced on the next day with approximately 3 × 105 plaque-forming unit (PFU) of lentiviral particles in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich, Steinheim, Germany). After 24 hours ...
-
bioRxiv - Cancer Biology 2020Quote: ... 48 hour supernatants were centrifuged and viral pellets were resuspended in 0.45 µm filtered 72 hour media and supplemented with 8 µg/mL (EMD Millipore, Burlington, MA, USA) added to either SW872 or NIH 3T3 cells overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2.105 Met-5A cells were plated in Ibidi µ-Slide 8 Well (Clinisciences #80826) coated with collagen type I (Sigma-Aldrich #C3867-1VL) and incubated for 48h at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Three days after the SVF media was replaced with media from the transfected 293T cells that was filtered and mixed at a 1:1 ratio with standard SVF media and viral media with added polybrene (8 µg/mL, Millipore Sigma TR-10030). The infected cells were selected for using Geneticin (G418 Sulfate ...