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Citations for Millipore Sigma :
4151 - 4200 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Physiology 2023Quote: ... Either pure Schneider’s (“mock”) or Schneider’s containing human insulin (Sigma-Aldrich #I9278, final concentration, 0.5 μM) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by single dose 120 mg/kg Abraxane (Royal Marsden Hospital) or human albumin control (Sigma) the next day ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Microbiology 2023Quote: ... plates were incubated with a secondary antibody (anti-Human IgG-Phosphatase alkaline antibody (reference: A9544, Sigma)) ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Bioengineering 2023Quote: ... For encapsulation and cell experiments a human hemoglobin in ferrous form was used (H0267, Sigma Aldrich), referred to as HbA0 throughout manuscript.
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bioRxiv - Immunology 2023Quote: ... resuspended in serum-purified human polyclonal IgG or IgA (I2511-10MG and I4036-1MG, Sigma Aldrich), and incubated at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Inserts were previously coated with 50 µl of human collagen IV at 30 µg/ml (Millipore) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and the oocytes were matured in vivo with 5 U of human chorionic gonadotropin (hCG; Sigma) before mating with B6D2F1/J males (F1 hybrid of a cross between C57BL6/J and DBA2/J ...
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bioRxiv - Cell Biology 2022Quote: ... per 1 ml fibrinogen at 1.5 mg/mL (plasminogen-Depleted from human plasma, Sigma-Aldrich #341578) in 1x Hanks’ buffered salt solution (HBSS ...
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bioRxiv - Bioengineering 2023Quote: ... laevis females were injected with 400 µL of human chorionic gonadotropin (2000 IU/mL, Sigma-Aldrich) and incubated for more than 16 h to induce ovulation in an incubator at 16°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were re-plated onto collagen (2.4ug/ml) (Type IV from human placenta Sigma Cat# C5533) coated plates in E8 with 10uM ROCK inhibitor (ROCKi ...
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bioRxiv - Cell Biology 2023Quote: Human VSMCs were isolated as previously described90 and were cultured in Dulbecco’s modified Eagle medium (Sigma) supplemented with 20% fetal calf serum ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Anti-human-DLL4 (Rabbit) and anti-LAM (rabbit) were from Sigma Aldrich (St. Louis, MO, USA). Anti β-ACTIN (rabbit) ...
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bioRxiv - Cell Biology 2023Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum (Sigma) and MCSF (10 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... flasks and plates were pre-coated with 10 µg/mL of human plasma fibronectin (Sigma-Aldrich) for 1h at room temperature before washing three times with water ...
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bioRxiv - Molecular Biology 2023Quote: ... Human primary fibroblasts were cultured in DMEM high-glucose with 1mM sodium-pyruvate (Sigma-Aldrich, # P5280) supplemented with 20% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the sequence of the shRNA targeting 3UTR of human NRF2 gene was obtained from Sigma Aldrich (TRCN0000007555 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used for im-munofluorescence in human cells: Mouse anti-G-Tubulin (Sigma T6557 ...
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bioRxiv - Immunology 2024Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Cell Biology 2024Quote: PC3 human prostate cancer cells (ATCC, CRL-1435) were cultured in HAM F12 medium (Sigma‒Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were grown on vitronectin coated plates containing PluriSTEM® Human ES/iPS Cell Medium (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Pathology 2024Quote: ... the cells were cultured on culture dishes coated with 0.05mg/ml human placenta derived COLIV (Sigma) in Bronchial epithelial cell growth medium with added supplements according to the manufacturer’s recommendation (BEBM-Lonza ...
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bioRxiv - Cancer Biology 2024Quote: Tumors were harvested as mice reached human endpoint and fixed in neutral buffered formalin (Sigma-Aldrich) for 24-48h ...
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bioRxiv - Cell Biology 2024Quote: MISSION shRNAs against human KIF1A (SHCLNG-NM_004321) and KIF5A (SHCLNG-NM_004984) were obtained from Sigma-Aldrich. Scramble vector was the MISSION pLKO.1-puro non-Mammalian shRNA Control vector ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/mL dsDNA or 10 μg/mL recombinant human insulin (Sigma Aldrich, St. Louis, MO). After five washes in PBS with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: Glycated Aβ (gAβ) was prepared by incubating 10 mg/ml of human Aβ1-42 (Sigma-Aldrich) with or without 0.5 mM methylglyoxal (MGO ...
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bioRxiv - Microbiology 2024Quote: Primary human foreskin fibroblasts (HFFs) (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) with 4.5 g/L glucose ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed and incubated with HRP-conjugated goat anti-human IgG antibody (Sigma-Aldrich) (diluted 1:2500 in PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: 50,000 human iPS-CM were seeded/well in Lab-Tek 4-well chamber slides (Sigma-Aldrich) and cultured for five days according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Human embryonic kidney 293 (HEK293) cells were cultured in RPMI-1640 medium (Sigma, St. Louis, MO) with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... HGF concentration in the CM was determined using the human HGF ELISA Kit (Sigma-Aldrich #RAB0212) following manufacturer protocol.
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bioRxiv - Cell Biology 2020Quote: ... At the end of the 3-hour incubation period cells were washed 3x PBS and lysed for western blot in lysis buffer (20 mM Tris pH 8, 10% glycerol, 135 mM NaCl, 0.5% NP40, 2.5 mM MgCl2, DNAse (Sigma-Aldrich, cat# DN25-1G), 1x protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... and glucose uptake was initiated by adding 2 ml serum-free and glucose-free DMEM containing 2-[1,2-3H(N)]-2-deoxy-D-glucose (DG) (NET328, specificity 8 Ci/mmol) at 1µCi/ml and 10 mM unlabeled-2-DG (Sigma, Catalog #: D-3179) to each sample in the absence and presence of 20 µM cytochalasin B (Sigma Catalog # ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Neuroscience 2020Quote: R6/1 mice were treated (from 8 to 20 weeks of age) with vehicle (90% water, 10% Polysorbate 80) or betulinic acid (50 mg/kg; Sigma-Aldrich #855057) administered by oral gavage ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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bioRxiv - Genetics 2019Quote: ... Western blots were probed using anti-GFP (Ozyme; #JL-8; 1/1,000) and anti-tubulin (to confirm equal loading) (Sigma, #DM1A, 1/5,000) antibodies ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Neuroscience 2019Quote: ... treatment consisted of an intraperitoneal injection of either the TrkB agonist 7,8-Dihydroxyflavone (7,8-DHF) (98% Sigma Aldrich CAS#: 38183-03-8) (10mg/kg ...
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bioRxiv - Neuroscience 2019Quote: ... well-fed L3 worms were mounted on 1% agarose pads with 5 mM sodium azide (Sigma-Aldrich, CAS No. 26628-22-8), whereas dauers were mounted on pads with 9 mM sodium azide (Simpkin and Coles ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Cell Biology 2020Quote: ... The following day cells were treated for 8 h with either ES DMEM-2i + DMSO or ES DMEM-2i + CHX (100 ug/ml (Sigma-Aldrich, 01810)) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1-hTERT cells were transduced with the viral supernatant from the transfections in the presence of 8 ug/mL polybrene (Millipore, Burlington, MA) for 24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the same amount of suspended cells were added into upper chambers of Corning Costar Transwell cell culture inserts (pore size 8 µm; Sigma-Aldrich, #CLS3464) with the coating of 30 μg MatrigelTM Basement Membrane Matrix (BD Biosciences) ...