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Citations for Millipore Sigma :
4201 - 4250 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... mixtures consisted in 2 μl of diluted template and 8 μl of FastStart Universal SYBR Green Master (Rox) (Cat. # 04913914001, Sigma-Aldrich Argentina). Reactions were set in technical duplicates or triplicates in MicroAmp® Fast Optical 48-well 0.1 mL reaction plates (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... animals at 8 weeks of age were gavaged with 100 µL of a 30 mg/mL solution of tamoxifen/corn oil (Sigma Aldrich T5648). This was done 3 consecutive days then animals were allowed to rest for 4 days before another three consecutive daily doses (6 doses over 10 days) ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Biophysics 2020Quote: ... 304 μL BPTI (4 different concentrations ranging from 5.2 to 52.6 μM) was mixed with 8 μL of the substrate Z-GPR-pNA (Sigma-Aldrich, St. Louis, MO) (5 different concentrations ranging from 0.4 to 10 mM) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant containing lentiviral particles was filtered through 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
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bioRxiv - Neuroscience 2021Quote: Microtissues were fixed within the agarose-molded wells of the tissue culture plate with cold PFA solution (4%(w/v) paraformaldehyde, 8% (w/v) sucrose) (F79-500, Sigma-Aldrich) (S7903, Sigma-Aldrich). Microtissues were fixed for 2 hours followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in a lysis buffer (20 mMTris-HCl pH=8; 137 mMNaCl; glicerol 10%) supplemented with protease inhibitor (Sigma-Aldrich, USA), and then centrifuged at 10.000g for 15min to the subsequent supernatant storage in −80°C ...
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bioRxiv - Neuroscience 2020Quote: ... Hair cells were inactivated by the flushing of 8-10 drops of 100 mg/mL neomycin sulfate (Sigma-Aldrich, St Louis, MO) through the punctured round window membrane whilst aspirating from the apex via a small hand-drilled hole ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were obtained from B6D2F1 female mice (8–10 weeks old) 48 hours after PMSG injection and were then transferred to M2 medium (Sigma-Aldrich, M7167) supplemented with 0.2 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Immunology 2019Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8-12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Immunology 2020Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8–12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Pathology 2020Quote: ... deletion of CCN2 or STOP in reporter td Tomato using Acan CreERT2 was induced at 8 weeks of age using tamoxifen (Sigma Aldrich, UK). All mice ...
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bioRxiv - Biophysics 2020Quote: ... The otolithic membrane was gently removed after 8-min enzymatic dissociation with 50 μg/mL Collagenase IV (Sigma Aldrich, St. Louis, MO). For experiments in which calcium concentration of endolymph was varied ...
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bioRxiv - Cancer Biology 2021Quote: ... we used a Boyden chamber which consists of cell culture insert containing 8-μm pore polyethylene terephthalate membrane (EMD Millipore, Burlington, MA). The inserts were seated in 24-well companion plates ...
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bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
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bioRxiv - Developmental Biology 2020Quote: Fetal hearts were collected at E11 from uninfected and CVB3 infected (E9) dams and cultured on Millicell EZ 8-well slides (Millipore Sigma PEZGS0816) in high-glucose DMEM (ATCC 30- 2002 ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...
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bioRxiv - Physiology 2021Quote: ... and used for infection into three breast cell lines in the presence of 8 μg/ml Polybrene (Sigma-Aldrich, St. Louis, MO). Infected cells were selected with 1.5 μg/ml puromycin (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... mixed in 1x PBS (8 ml per 10 mg) prepared from PBS tablets (Dot Scientific, Michigan) with deionized distilled water and filter sterilized (Millipore, Burlington, MA). Control animals were injected with an equivalent volume of saline made of 1x PBS ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Pathology 2022Quote: ... Then the leaves were soaked up using absorbent paper and transferred into another square Petri dish containing 8 g L-1 agar with 50 mg L-1 benzimidazole (Sigma-Aldrich, USA) for 24 hours and finally transferred to new Petri dishes and infected with Bgt #70.
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 mL of filtered virus particles were added to the cell suspension of 50,000 cells in the appropriate media supplemented with 10% FBS and 8-10 μg/mL polybrene (Sigma # TR-1003-G) (without any antibiotics ...
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bioRxiv - Neuroscience 2022Quote: ... The recombination was induced in 8-weeks old animals by intraperitoneal (i.p.) injection of 1 mg tamoxifen (100 μl; Sigma-Aldrich, Buchs, Switzerland), dissolved in sunflower seed oil/ethanol (10:1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
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bioRxiv - Neuroscience 2022Quote: ... −1.8 mm from cortical surface) using glass microcapillary (tip diameter ≃35 μm; 1–5 μL Hirschmann® microcapillary pipette, Z611239, Sigma-Aldrich) connected to a custom Openspritzer pressure system (Forman et al. ...
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bioRxiv - Microbiology 2024Quote: ... the dried protein pellet was dissolved in 50 µL of 8 M urea (Cytiva, 17131901) in 100 mM NH4HCO3 (ABC; Sigma-Aldrich, 11213) and 5 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was either digested for 4 min for nChIP or 8 min for ChIP-seq with 1.0 U MNase (Sigma Cat #N3755-500UN) in nuclei solubilized with 1 mL of 0.1 M TE (10 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells at 70-80% confluence were infected with the viral supernatant supplemented with 8 μg/ml polybrene (Sigma: TR-1003-G). Infected cells were selected using puromycin (Meilunbio ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... 20-30 μg of protein for each sample was separated by 8-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore, cat# IPVH00010) using Trans-Blot Systems (Bio-rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing lentiviral particles was filtered through a 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Developmental Biology 2022Quote: ... WT/IWR consists of 9 wild type embryos and 8 embryos treated with the small molecule inhibitor 10µM IWR1 (Sigma Aldrich, Cat I0161). DMSO/DMH4 includes 12 embryos at 3 dpf ...
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bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
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bioRxiv - Pathology 2023Quote: ... Cells were infected with lentivirus carrying shHDAC6/shATG5 or shControl at MOI 5 for 24 hours in the presence of Hexamethidine Bromide (8 µg/mL; Sigma-Aldrich - H9268). Transduced cells were selected using puromycin (Sigma Aldrich #P9620 ...
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bioRxiv - Microbiology 2023Quote: Cellvis 8-well (Mountain View, CA, USA) coverslips were pre-coated with laminin (20 µg/mL, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 33 °C and washed twice with ddH2O ...
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bioRxiv - Molecular Biology 2023Quote: ... sonicated for 2 minutes of 2 sec on/8 seconds off cycles on ice using probe sonicator (Ultrasonic processor 500W, Sigma Aldrich, USA). 3-5 mL of 5 % NaCl was added to the samples and after thorough vortexing the phases were separated by centrifugation at 3,000 × g for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated for two weeks (treatments replenished at days 4, 8 and 11) prior to addition of 0.5% crystal violet (Sigma-Aldrich, Cat#C0775) in 50% methanol for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene_17446)] at a multiplicity of infection of 10 with 8 μg/mL polybrene (hexadimethrine bromide [Cat # 107689, Sigma Aldrich, St. Louis, MO, USA) for 24 hours before washing with PBS and replacing with fresh medium (49) ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Genomics 2023Quote: ... large tissue pieces were spun down in a microcentrifuge and the supernatant containing single cells was passed through a 70-um cell strainer fitted onto a 50-mL conical tube containing 8 mL DPBS + 1% bovine serum albumin (BSA; Sigma-Aldrich, A7906) in DPBS on ice ...