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Citations for Millipore Sigma :
4051 - 4100 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
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bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Biophysics 2023Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) dry powders were purchased from Sigma Aldrich (St. Louis, MO, USA). Different amounts of DOPC and DOTAP were used to tune the surface charge of the employed liposomes ...
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bioRxiv - Biophysics 2023Quote: ... the quench buffer also included 3 μL of 0.8% GDN and 3 μL of 300 mg/ml aqueous suspension of ZrO2-coated silica (Sigma-Aldrich, reference no. 55261-U). The resulting mixture was incubated on ice for 1 min to remove lipids and solubilize prestin in GDN before being filtered through a cellulose acetate spin cup (Thermo Pierce ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were blocked in a blocking solution including 1% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated in blocking buffer with primary antibody (Rabbit α-β-III Tubulin, Millipore Sigma T2200-200UL, 1:1000, RRID: AB_262133) overnight at 4°C without agitation ...
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bioRxiv - Neuroscience 2021Quote: The other two remaining free-floating brain sections were washed in 0.1% PBS with 0.1 % Triton A-10 (PBS-T) and incubated in blocking solution (1 h at room temperature; PBS-T with 10% donkey serum, Sigma Life Science). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was probed overnight at 4°C with blocking buffer containing rabbit anti-Extramacrochaetae antibody and an antibody to β-actin (Sigma-Aldrich). The membrane was then washed four times with 1X PBST for five minutes each ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were incubated overnight with the following antibodies diluted into blocking solution: chicken anti-GFP (1:1,000; AB3080; Millipore Bioscience Research Reagents), rabbit anti-RFP (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 1 hour at RT with the following antibodies diluted in blocking buffer: fibronectin (Sigma-Aldrich, St. Louis, MO) and α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... These sections were placed in a blocking buffer (2% normal donkey serum and 1% bovine serum albumin in PBST; Sigma A7906-100G) for 30min ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibodies in blocking buffer in a humidified chamber for 1 h: anti-FLAG M2 (1:2000 dilution, Sigma Aldrich, F3165) and an endogenous compartment marker antibody from rabbit (Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and then blocked for 1 h at 37 °C with blocking buffer containing 1% BSA (Sigma-Aldrich, Taufkirchen, Germany) and 0.15M sucrose (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue samples were blocked for 1 hour at room temperature in blocking solution (8% BSA solution) and then transferred to a carrier solution (8% BSA solution) containing diluted antibodies: GOT2 (Sigma-Aldrich HPA018139), GOT2 (Thermo Fisher PA5-77990) ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 hour of blocking with 5% horse serum in PBS was followed by incubation with anti-FLAG primary antibody (1:500, Sigma Aldrich F3165) in 3% horse serum in PBS for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... free floating sections were incubated with mouse anti-FLAG clone M2 antibodies (1:2000 in the donkey serum blocking solution, Sigma-Aldrich F1804), overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1 hr with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidity chamber at RT for 1 hour with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2021Quote: ... and iii) blocking with 5% Bovine Serum Albumin (BSA) and 0.5% Triton-X in Phosphate Buffer Saline (PBS, Sigma-Aldrich ref. P4417) for 30 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by incubation in blocking solution and primary antibody against ChAT (Millipore, AB144P, goat anti-ChAT, 1:500 v/v) for five nights at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... After incubating overnight in blocking solution containing a primary antibody (polyclonal rabbit anti-tyrosine hydroxylase; 1:1000 dilution; Millipore; catalog no. 657012), sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG ...
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bioRxiv - Pathology 2020Quote: ... the coverslips were incubated in blocking buffer for 45 min at room temperature with antibodies against LC3-II (Sigma Aldrich, 1:200), LAMP2 (DSHB ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibody was diluted 1:100 in blocking buffer (rabbit-α-LAMP1: 1:100; rabbit-α-mTOR (HPA071227, Sigma-Aldrich Co. LLC): 1:12.5 ...
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bioRxiv - Genetics 2020Quote: ... rinsed and then pre-incubated with blocking solution (10% Normal Goat Serum (Brunschwig, #G-S-1000) and 2% Bovine Serum Albumin (Sigma-Aldrich, #A7906) in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Pathology 2021Quote: ... Antibody solution consisted of blocking solution with rabbit anti-Iba1-635 (Wako, cat. # 013-26471) at 1:400 and DAPI (Sigma, cat. # D9542) at 10 µg/mL.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM reporter probe DNA and 30 μl of 100 μM blocking probe sequences (PolyT10) thiolated DNA were activated by using 10 mM Tris (2-carboxyethtl) phosphine (TCEP, Sigma-Aldric, USA) freshly prepared ...
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bioRxiv - Immunology 2020Quote: ... Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4], 0.1% bovine serum albumin [BSA; Sigma], and 0.05% Tween 20) at 37°C to block the unbound sites and the antigenic integrity of Env proteins were assessed by examining their ability to bind to different mAbs by flow cytometry (FACSCanto ...
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bioRxiv - Biophysics 2022Quote: ... Solutions of primary antibodies were prepared in blocking solution using the following dilutions: mouse antibody against SRRM2 (1:2000, Sigma Aldrich, #S4045), mouse antibody against SRSF1 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: Embryos were incubated in 600 μl of blocking buffer (PBS, 0.01% Triton X-100 and 10% non-immune donkey serum (Sigma cat # D9663-10ml) overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Permeabilization of the membrane and blocking of unspecific binding sites were performed in one step by adding 0.5% Triton X-100 (Sigma-Aldrich, Buchs, Switzerland) to the blocking solution (PBS - Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cells were quenched in 0.1 M glycine in PBS and then permeabilized for 30 min in a blocking solution of 2 % normal goat serum (Sigma-Aldrich, # G9023) and 0.1 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... an overnight incubation was performed to label the peroxisomes with anti-PMP70 (Cat#P0497, Sigma-Aldrich; 1:1000 dilution in blocking solution). The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034 ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich] ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated for 2-hour incubation with a blocking solution at room temperature and subsequently incubated with anti-digoxigenin-Rhodamine (Anti-digoxigenin-Rhodamine, Fab fragments, Sigma, Cat# 11207750910) (0.5μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... and the plate was blocked with 200 µL blocking solution (1% BSA [Sigma, #A9418-5G], 0.05% Tween [Sigma, #P9416-100ML], in PBS). The solution was removed and sample or standard curve (made with mouse IFN-β [PBL Biomedical Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...