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Citations for Millipore Sigma :
3951 - 4000 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Immunology 2021Quote: ... The beads were then washed with IP buffer three times and incubated in FLAG-elution buffer (100 μg/mL FLAG peptides (Sigma), 50 mM Tris-HCl pH7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were eluted in Laemmli buffer by boiling at 95°C for 5min for Western blot or with 3xFlag peptide (150 µg/ml, Sigma) for 1 hr at 4°C for HAT assay ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 4°(C) Immunocomplexes were washed 5× with S300 buffer and eluted by competition with 1x Flag peptide (500 ng/ul; SIGMA) 2 times for 30 min at 16 °C and then resuspended in Laemmli sample buffer ...
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bioRxiv - Immunology 2019Quote: ... Commercial sources were used to obtain biotinylated CD16 (Acro Biosciences) and the biotinylated 19-mer adjacent loop peptides that overlap by 16 amino acids (Sigma). Loop peptide 1 is KDQQLLGIWGCSGKLICTT and loop peptide 2 is QLLGIWGCSGKLICTTAVP.
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bioRxiv - Immunology 2020Quote: ... Beads were eluted three times at room temperature for 15 min each using Influenza Hemagglutinin (HA) peptide (Sigma Aldrich I2149).
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bioRxiv - Cell Biology 2019Quote: ... Beads were washed extensively in lysis buffer and bound proteins eluted in 500 µL 100 µg/ml 3X FLAG peptide (Sigma) in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... were plated in 96-well plates (flat bottom, Costar) and pulsed with the indicated amounts of Melan-A peptides (ELAGIGILTV, Sigma) for 2 hours at 37C ...
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bioRxiv - Immunology 2021Quote: ... plated at 2 × 106 cells/ml in cDMEM and rested overnight at 37°C in 7.5% CO2 following which BMDCs were stimulated overnight with Ag85B (20 µg/ml) (New England Peptide) and Zymosan (25 µg/ml) (Sigma) to induce maturation and activation ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were extruded from the gel pieces by 15 minutes sonication and desalted using C18 ZipTips (Merch Millipore, Darmstadt, Germany), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... were resolved on a 12.5% SDS gel and puromycin-labelled peptides were detected with anti-puromycin (1:5000 mouse anti-puromycin, clone 12D10; Sigma). Prior to antibody detection ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Neuroscience 2020Quote: ... These were formed by 24h incubation at 37°C of a 1mg/ml solution of Aβ 1-42 peptide (Sigma) in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... elution was performed three times each using 20 μL of a 150 μg/μL stock of 3xFLAG peptides (Sigma Aldrich). Western blot analysis was done as previously described [7] ...
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bioRxiv - Plant Biology 2020Quote: ... The coding sequence of PhS3-RNase (without signal peptide) as well as its six mutant forms described above were separately cloned into engineered pET-30a (Novagen) containing N-terminal SUMO tag to produce SUMO-His-tagged proteins ...
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bioRxiv - Plant Biology 2020Quote: The coding sequences of PhS3-RNase (without signal peptide) and its six mutant forms were separately cloned into pMAl-c2x (Novagen) to generate MBP-tagged fusion proteins ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged protein bound to RNA was eluted by incubation with 100 μL of FLAG elution solution (150 ng of 3X FLAG peptide per μL) (Sigma) for 30 min at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
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bioRxiv - Microbiology 2021Quote: ... Aminopeptidase activity was assessed by fluorescence assays using the fluorogenic peptide L-leucine-7-amido-4 methylcoumarin hydrochloride (Sigma L2145) as a substrate as previously described (19) ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were immediately used for measurement of proteasome peptidase activity by cleavage of the fluorogenic peptide N-Suc-LLVY-MCA (Sigma), enzymatic activity were acquired for 2h ...
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bioRxiv - Microbiology 2020Quote: ... the bound proteins were eluted with 400 μL of wash buffer supplemented with 250 μg/mL HA peptide (Sigma-Aldrich) by incubation at room temperature for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: Translated peptides were purified from in vitro translation reactions by enrichment using Anti-FLAG® M2 Magnetic Beads (Millipore Sigma) according to the manufacturer’s protocol with slight modifications ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabeads following the first pulldown were resuspended in Re-ChIP elution buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 100 µg of 3X FLAG peptide [Sigma, #F4799]), and incubated in 4 °C for 1 hour with rotation to selectively elute FLAG-fusion protein ...
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bioRxiv - Molecular Biology 2022Quote: ... FLAG-tagged proteins were eluted by 15 minutes shaking (500 RPM) in 25 μl of B100 + HALT containing 1 mg/ml 3xFLAG peptide (Sigma) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... glutathione or flag peptide were removed by buffer exchange and the purified proteins were concentrated on Amicon Ultra 50K filters (Millipore). Purified proteins were recovered in 20 mM Hepes ...
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bioRxiv - Cell Biology 2022Quote: ... a2 and a3 spliced-variants of the Pde5 gene fused at their 5’ end to the short DNA sequence coding for the 3XFLAG peptide (Sigma) [20] ...
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bioRxiv - Neuroscience 2020Quote: Amyloid-beta protein fragment 1-42 (Aβ42 peptide) was purchased as a lyophilized solid and more than 95% purity was guaranteed by HPLC from Sigma-Aldrich (High Performance Liquid Chromatography) ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Genetics 2021Quote: ... NMDA receptor subunit 2 C-terminal peptide “SIESDV” was synthesised (Pepceuticals) and fully dissolved in 90% v/v methanol + 1M HEPES pH7 (both Sigma). Dissolved peptide was coupled to Affi-Gel 10 resin (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then eluted off the anti-Flag beads using competitive 500 µg/mL 3xFlag peptide (Sigma-Aldrich, F4799-4mg) elution in 150 µL lysis buffer by rotation for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins in the insoluble cell wall fraction were digested by trypsin and peptides and glycopeptides were desalted using C18 ZipTips (Millipore).
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were recovered by centrifugation followed by two additional washes with 50mM ammonium bicarbonate and 0.5M NaCl (Sigma-Aldrich, Switzerland). The two filtrates were combined ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then the elution buffer with the targeted modified peptide was replaced with PB buffer using an Amicon Ultra centrifugal filter (Millipore). Trypsin (2.5% ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Systems Biology 2019Quote: ... These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled selected reaction monitoring ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing target proteins were combined and concentrated using an Amicon Ultra-15 Centrifugal Filter Unit (10 kDa for precursor peptide, 30kDa for modification enzymes, MWCO, Millipore). The resulting protein sample was stored at −70 °C.
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bioRxiv - Biophysics 2021Quote: ... Between 0.25 to 0.5 mL of a 100-200 ng/mL solution of FLAG peptide (Sigma-Aldrich, St. Louis, MO) in KBT was added to the rinsed beads ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Biophysics 2021Quote: The peptide GAL was purchased from USV Limited (Mumbai, India) by custom synthesis and other chemicals were obtained from Sigma Chemicals or other sources with the highest purity available.
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bioRxiv - Immunology 2020Quote: ... overlapping 15mer peptides offset by 11 residues were designed and synthesised (Mimotopes) and reconstituted in sterile 100% DMSO (Sigma-Aldrich) at a concentration of 100 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were manually dissociated through a 70µM cell strainer and OTI T-cells were activated and expanded with 20ng/mL of SIINFEKL peptide (Sigma-Aldrich) and 100 IU/mL recombinant human IL-2 (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... STM14_0336 was amplified by PCR and cloned into pBRA vector under the control of PBAD promoter (M. Marroquin, unpublished) with or without PelB signal peptide sequence from pET22b (Novagen) (Bayer-Santos et al. ...