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Citations for Millipore Sigma :
3901 - 3950 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent to detect Pax7 (Developmental Studies Hybridoma Bank, 1:10) and laminin (Sigma, 1:50). After 16h incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking, cells were incubated with anti-GLUT4 (LM048) (2 µg/mL, Integral Molecular, PA, USA) and Lectin-FITC conjugate (Sigma #L4895) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were subsequently blocked for 1 hour at room temperature in blocking buffer (PBS with 5% skimmed milk powder (Sigma-Aldrich) and 0.1% Tween) ...
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bioRxiv - Cell Biology 2023Quote: ... Specimens were washed with phosphate-buffered saline and blocking solution (1% BSA, 0.1% gelatin from cold-water fish skin (Sigma-Aldrich G7041), 0.5% Triton X-100 and 0.05% Tween-20 (Sigma-Aldrich P1379 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in the blocking solution (5 % Normal Goat Serum, 0.1 % Triton-X, 0,1 % DMSO in PBS) (Sigma Life Science, 1002135493) for at least 1 hour before incubating in the primary AB containing-blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos after in situ HCR were washed with PBST and soaked in blocking solution [5% heat inactivated (56°C, 30 min) sheep serum (S2263, Sigma-Aldrich) in PBST] at room temperature for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were incubated ON at 4 °C with primary antibodies diluted in blocking solution supplemented in 0.05 % Tween-20 (Sigma-Aldrich Corp.); anti-HSP70 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Once dried, coverslips were placed upside down on a drop of hybridizing solution (70% formamide, 0.5% blocking reagent (Sigma, Cat:11096176001), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... collagen and mineralized collagen in the presence and absence of a function-blocking β1 integrin antibody (2 µg/mL, Millipore, US) or pharmacological inhibitors of focal adhesion kinase (FAK ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing 3x in ddH20 slides were blocked for 1 hour with blocking solution: 5% normal donkey serum (Sigma, Cat#D9663), 0.1% Tween-20 in PBS ...
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bioRxiv - Systems Biology 2023Quote: ... 50 mL of blocking buffer was prepared per 2 x 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization and blocking for 1 hour at room temperature in PBS with 0.5% Triton-X-100 (Sigma #X100-500ML)/0.2% Tween-20 (Sigma #P1379)/2% BSA (Sigma #A7906) ...
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bioRxiv - Immunology 2023Quote: ... Blocking was performed in 5% non-fat dry milk in PBS containing 0.2% Tween 20 (Sigma-Aldrich, St. Louis, Missouri, USA). Membranes were incubated in primary antibodies for 1-3 h at room temperature (20-25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells where thrice washed in PBS then incubated in blocking buffer (5% Donkey Serum (Jackson Immuno Research Labs 017-000-121, 1% BSA (Sigma A9647), 0.1% Triton X-100 (Sigma T8787 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated overnight at 4°C with the primary antibody diluted in blocking solution (5% goat and/or donkey serum in PBS, #G9023 and #D9663 Sigma-Aldrich). Cells were washed with complete PBS 3 times for 5min at RT and then incubated with the following secondary antibodies for 1h at RT ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed twice more with 0.01M PBS before blocking in 5% (v/v) normal donkey serum (NDS; catalogue # S30-100ml, Sigma Aldrich, UK) in 0.01M PBS (3% Tween-20 ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated for 1-2 h at room temperature in PBST with blocking agent (5 mg/mL Bovine Serum Albumin (Sigma Aldrich) for anti-Strep blots ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma], and 0.1% Tween-20 [P9416; Sigma] in PBS) for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then incubated for 30 min at 37°C in blocking buffer [0.5% (w/v) bovine serum albumin (BSA, Sigma Cat. #A9647), 10% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Immunology 2021Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Sigma), developed with pNPP SigmaFAST substrate and absorbance was read with a 405nm – filter (Figure 3D-F).
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bioRxiv - Molecular Biology 2021Quote: ... The beads were then washed three times for 5 min with mild EJC-Buffer before eluting twice with 22 μl of a 200 μg/ml dilution of FLAG-peptides (Sigma) in 1x TBS for 10 min at RT and 200 rpm each elution step ...
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bioRxiv - Neuroscience 2020Quote: ... mice were administered murine MOG 35-55 (Johns Hopkins Peptide Synthesis Core Facility, Baltimore) in incomplete Freund’s adjuvant (Sigma-Aldrich) supplemented with heat-killed mycobacterium tuberculosis (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...