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Citations for Millipore Sigma :
3851 - 3900 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Free-floating tissue sections were washed three times for 10 minutes with PBS then incubated for 2 h in a PBS-blocking solution containing 0.5 % Saponin (Sigma Aldrich 84510) and 10 % pre-immune goat serum (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma, I4506). Free active groups were blocked overnight with 0.5M Tris (pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blocking was followed by an overnight incubation at 4°C with antibodies against α-smooth muscle actin (αSMA) (clone 1A4, Sigma), Bip (ab21685 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight in blocking solution containing one of the following antibodies: Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152), Anti-Iba-1 (1/1000 ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated ON at 4°C with the following primary antibodies diluted in blocking buffer: anti-FLAG (1:200, M2 clone, catalog no. F1804, Sigma-Aldrich); anti-FLAG (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1h with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blots were blocked with LI-COR Blocking Buffer for one hour at room temperature and then incubated for two nights with primary antibody (GFAP GA5 Sigma 63893) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed tissues were rinsed three times with PBST before incubation in blocking buffer (PBS with 5% goat serum; Sigma Aldrich, #G9023) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Licor Blocking buffer (Odyssey, Lincoln, NE) for 1h at room temperature and primary antibody (Kal-spec, Millipore Sigma, Burlington ...
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bioRxiv - Immunology 2021Quote: ... antibody diluted 1:1000 in blocking solution or rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody (Sigma-Aldrich Cat# A2054, RRID:AB_257967) were added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated in blocking buffer containing 2.5% bovine serum albumin and 0.1% Triton X-100 (Sigma Aldrich, St Louis, MO) for 30 minutes at room temperature then incubated in a primary antibody dilution in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with primary antibodies in blocking buffer for 1h at 21°C: 1:1000 α-tubulin (118M4779V; Sigma Aldrich), 1:250 CREST (15-234-0001 ...
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bioRxiv - Immunology 2022Quote: ... Fixed cells were washed twice with PBS and the remaining PFA was inactivated with blocking buffer (PBS-2% BSA (Sigma: #A9418), 1.5M Glycine (Sigma ...
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bioRxiv - Pathology 2020Quote: ... The blocking solution used for the anti-phospho-Smad2 and anti-phospho-Smad3 antibodies contained 5% bovine serum albumin (Sigma-Aldrich). The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2020Quote: ... after several washes in PBS explants were incubated at room temperature for 1 hour in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and 1:400 for TRITC-conjugated phalloidin (Sigma-Aldrich)) and then mounted using Fluoromount-G (Southern Biotech).
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Genomics 2019Quote: ... Cells were incubated with primary antibody diluted in the blocking buffer for 2 hours [alpha-tubulin mouse mAb (1:5000, Sigma T6199), Rad21 rabbit pAb (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
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bioRxiv - Cell Biology 2020Quote: ... the channel was washed three times with 10 μl nuclease-free water and the channel walls were passivated with 10 mg/ml Western Blocking Reagent (Sigma Aldrich) in PBS for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently incubated for 1 hr at room temperature in a blocking solution consisting of PBS with 5% Goat Serum (Sigma-Aldrich). Following blocking ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic protein G beads were washed with blocking solution (0.5% BSA in PBS) and then incubated with rabbit antiserum against acetylated histone H4 (Millipore 06-866) or normal rabbit anti-serum in blocking solution for several hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were blocked in blocking buffer (5% skim milk, 0.1% Tween in PBS) and incubated with β-Actin (#A5316, Sigma-Aldrich), MYC (#9402 ...
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bioRxiv - Cell Biology 2021Quote: ... the proportions of respiration driving ATP synthesis and proton leak were determined by blocking ATP synthase with 2 μM oligomycin (Sigma-Aldrich). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with the following cocktail of primary antibodies to presynaptic components diluted in blocking solution with rocking: rabbit anti-NF 200 (1:200; Sigma, N4142), mouse anti-synaptophysin (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with blocking and permeabilization solution (PBS containing 0.25% Triton-X100 (Cat#93443, Sigma-Aldrich, St Louis, MO, USA) and 3% bovin serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: Fixation was carried on in 4% PFA for 15 minutes and then the coverslips where incubated with a blocking/permeabilization solution (PBS-BSA 1%, 2mM Vanadyl ribonucleoside complexes (VRCs) solution (Sigma #94742), TRITON-X 0.3% ...
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bioRxiv - Immunology 2022Quote: ... Sections were then cleared using 10% w/v CHAPS and 25% v/v N-Methyldiethanoamine in PBS for 48hrs before washing with PBS followed by 72hrs of blocking 5% donkey serum (Sigma, #D9663) in PBS ...
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bioRxiv - Systems Biology 2022Quote: ... 50 mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex antibody cocktails were made in a whole-mount blocking solution consisting of 5% heat activated normal donkey serum (Sigma Millipore), 20% Dimethyl sulfoxide (Sigma Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked with 1 drop of blocking solution provided with the Duolink® PLA Probes (DUO92002 and DUO92004, Sigma-Aldrich) for 1h at 37°C in a humid chamber ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Trap®_MA beads were washed with low-salt buffer and blocked for 60 mins at room temperature in Casein Blocking Buffer (Sigma-Aldrich) supplemented with 50 μg/mL Heparin ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... We blocked the membrane for 1 hour with blocking buffer: 5% bovine serum albumin (BSA, BioShop) in tris-buffered saline (BioShop) with Tween 20 (Millipore Sigma) (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... preparations were washed for 1 hour in blocking buffer containing 5% (vol/vol) normal goat serum (NGS) (Cat# G9023, Sigma-Aldrich) in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking in LiCOR blocking buffer the following primary antibodies were used: DDX5 (Pab204, gift from Prof. Frances Fuller-Pace; clone204, Sigma-Aldrich), DHX9 (ab26271 ...
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Molecular identification of ALDH1A1 and SIRT2 in the astrocytic putrescine-to-GABA metabolic pathwaybioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibodies in blocking solution in the following composition: guinea-pig anti-GABA antibody (1:1000, AB175, Millipore, USA), chicken anti-GFAP antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were washed multiple times with PBST (0.8% Triton-X 100 in PBS) and incubated in blocking buffer (10% NGS (Sigma-Aldrich, #G6767) and 4% BSA (Sigma Aldrich ...