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Citations for Millipore Sigma :
4051 - 4100 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed 48 hours later by intraperitoneal injection of 5 IU human chorionic gonadotropin (SIGMA). Mouse zygotes were obtained by mating C57BL/6J males with superovulated C57BL/6J females at a 1:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma, USA). Cultures were maintained at 37°C in a gas mixture composed of 5% O2 ...
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bioRxiv - Immunology 2023Quote: ... encoding a gRNA for human AAVS1 targeting (GGAAGAGAGTAGGTCGAAG) using GeneJuice Transfection Reagent (Sigma-Aldrich). Transfected cells were selected with either 0.5μg/mL puromycin or 100μg/mL neomycin ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: Purified human NUDT15 (20 mg/mL) was preincubated with 10 mM GPP (Sigma-Aldrich) for 2 hours at 20 °C ...
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bioRxiv - Microbiology 2023Quote: ... Purified human IgG and IgA were used as standards (Sigma, St. Louis, MO, USA). Tear samples were tested by 4-fold serial dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific L3000015 ...
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bioRxiv - Bioengineering 2023Quote: ... human skeletal muscle biopsies were minced and incubated with collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with purified collagen I from human placenta (Sigma), gelatin from bovine skin (Sigma ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... Coverslips were blocked and permeabilized in staining buffer [1% human serum (Sigma Aldrich, 4552) heat-inactivated at 56 °C for 1 hr ...
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bioRxiv - Immunology 2024Quote: The MILLIPLEX MAP Human CD8⁺ T-Cell Magnetic Bead Panel (Merck Millipore Darmstadt, Germany) was used to quantify GM-CSF ...
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bioRxiv - Developmental Biology 2024Quote: ... followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG; Sigma-Aldrich). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... NSCs were validated for expression of mouse anti-human Nestin (1:2,000; Millipore; MAB5326), rabbit-anti Sox2 (1:400 ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... females were injected with 5 I.U of human chorionic gonadotropin (hCG) (Sigma-Aldrich #CG5) to induce ovulation ...
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bioRxiv - Genomics 2024Quote: All human mammary epithelial cell lines MCF-10A TP53+/+ and TP53-/- (Sigma-Millipore clls1049) were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Genetics 2024Quote: ... Spermatozoa were capacitated in Human Tubal Fluid medium (HTF) (Merck Millipore; MR-070-D) supplemented with 10 mg/ml Albumin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Human microvascular endothelial cells (HMEC-1) were cultured in MCDB-131 medium (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Bioengineering 2024Quote: ... Human hepatic stellate cell line LX-2 were acquired from Sigma (cat. no. SCC064), and were cultured in DMEM-2 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... The 22Rv1 human prostate cancer cell line was grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then quickly coated with 100 ug/mL human fibronectin (Sigma #SLCL0793) diluted in PBS and injected using a pipette tip ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Patu8988S and MiaPaCa-2 human pancreatic cancer cells were cultured in RPMI (Sigma, #R1383) or DMEM (Gibco ...
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bioRxiv - Immunology 2024Quote: Human cell lines: T47D (HTB-133, ATCC) were cultured in RPMI-1640 (R8758, Sigma) and supplemented with 0.2 unit/ml bovine insulin (I0516-5ML ...
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bioRxiv - Immunology 2024Quote: ... Human CD34+ cells were isolated from FL by density gradient centrifugation (Histopaque-1077, Sigma) followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were collected at appropriate time points from 5-8 week old hormone-primed (5 IU each, pregnant mare serum gonadotropin (Sigma) and human chorionic gonadotropin (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... cells following subjected treatments were collected and lysed in NP40 buffer (50mM Tris-HCl pH 8, 150mM NaCl, 5mM EDTA, 0.5% NP-40, 1x EDTA-free protease inhibitor (Sigma-Aldrich), 1x phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at a density of 1 x106 cells/mL in RPMI containing 8 μg/mL polybrene (EMD Millipore), and then transduced with lentivirus by centrifugation at 2,200 RPM for 45 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected on ice in 0.5% NP40 lysis buffer (50 mM tris-Hcl pH=8. 100 mM NaCl, 10% glycerol, EDTA-free protease inhibitor cocktail (Sigma), 10 μM of MG-132 (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug RNA was mixed with 0.025 pmole of tRNA standards (e.coli-tyr-tRNA and s.cerevisia-phe-tRNA at ration of 1:8 (Sigma-Aldrich; XX)) ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 55 million Jurkat cells seeded at 8 million cells ml-1 was treated with 50 µM of 4-thiouridine (Sigma) for 7 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Human fibroblasts were doubly transduced with either shRNA or CRISPR vectors in consecutive days in the presence of 8 µg/ml protamine sulfate (Sigma). Transduced cells were selected by puromycin at 1 μg/ml.
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bioRxiv - Molecular Biology 2022Quote: ... 0.5×106 cells were mixed in a well of a 12-well plate with varying concentrations of supernatant containing viral particles and 8 μg/ml of polybrene (Sigma) which was then centrifuged at 2,000 rpm for 30 minutes at 30°C.
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were transduced with equal volumes of freshly harvested LifeAct-mCherry and SIV3 lentiviral particles in the presence of 8 μg/mL of protamine (Sigma). At 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with ice-cold PBS and then lysed with freshly prepared lysis buffer composed of 8 M urea (Sigma), 0.1 M HEPES (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The harvested lentiviral supernatants were filtered using 0.45 μm syringe filter (Fisher) and used to infect target A549 cells (polybrene was added at a final concentration of 8 μg/ml, Sigma). To infect A549 cells for CRISPRi ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at a density of 1,000 cells per mm2 into 8-μwell plates (BioValley) coated with 50 μg/ml poly-D-lysine (Sigma Aldrich). Cultures were kept at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cell Biology 2022Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10 -8 to 3· 10 -5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Biophysics 2022Quote: ... of polyethylene glycol (PEG) of a range (0.4, 2, 4, 8, 20 or 35 kDa) of molecular weights (Sigma-Aldrich). GC376 (GC376 Sodium ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... mTEC were cultured from ALI day -3 to ALI day 8 with an added gamma-secretase inhibitor (DAPT, 50 μM, Sigma) or vehicle control (DMSO ...
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bioRxiv - Immunology 2022Quote: Monocytic THP-1 cells were infected at a density of 2×105 cells/ml in 48 well plates in the presence of polybrene (8 μg/ml, Sigma). Infection levels were assessed at 48 h post-infection through enumeration of GFP positive cells by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... cells were cross-linked with 1% fresh formaldehyde for 15 minutes and treated with lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% IGEPAL CA-630 (Sigma-Aldrich), 1X complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2019Quote: ... pH 8) containing 3 mg/ml lysozyme (Chicken egg white; Fluka) and 1 mg/ml lipase (from Candida cylindracea; Sigma) in an Eppendorf tube ...
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bioRxiv - Biophysics 2019Quote: ... A pre-reaction mixture was prepared in MTEN buffer (5 mM MES (Sigma Aldrich, cat# 69889, CAS: 145224-94-8), 25 mM ethanolamine (Sigma Aldrich ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...