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Citations for Millipore Sigma :
3851 - 3900 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected in lysis buffer (150mM NaCl, 50mM Tris-HCl pH 8, 1mM DTT, 0.5% NP-40, with protease and phosphatase inhibitors (Sigma).After 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors, 0.1% Triton X 100 Sigma) via dounce homogenization on ice ...
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bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: ... 8 mL of the modified live cell imaging solution supplemented with iodide and 20 μM forskolin (FSK, EMD Millipore; 20 mM HEPES buffer at pH 7.4 containing 40 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Cancer Biology 2022Quote: ... The ‘Positive Cell Detection’ function was used to identify Ki67+ and Ki67-cells using the following parameters: Nucleus Parameters (Requested pixel size 0.5 µm, Background radius 8 µm, Median filter radius 0 µm, Sigma 1.5 µm ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Molecular Biology 2024Quote: ... Target cells were transduced on two consecutive days by supplementing the lentiviral supernatant with 8 μg/mL polybrene (Sigma). One day after the final transduction ...
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bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 p53-/- Cas9 expressing DD-HA-ER-I-PpoI were transduced with the lentiviral TKOv3 library at a low MOI (∼0.30) in the presence of 8 μg/mL polybrene (Sigma)43,44 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were crosslinked for 8 min at room temperature in 10ml PBS supplemented with 1% formaldehyde (Sigma, F8775). Cross-linking was quenched by adding glycine to a final concentration of 0.125 mM and incubating at room temperature for 1min ...
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bioRxiv - Genomics 2024Quote: ... the embryos (8-16 cell stage) were washed through M2 and then placed in drops of Tyrode’s solution (Sigma). After about 30 sec ...
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bioRxiv - Molecular Biology 2024Quote: ... the mice were subcutaneously injected with vitamin D3 (6 or 8 x 105 IU/kg; C1357, Sigma-Aldrich, USA) daily for three days (n=5-8 per group) ...
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bioRxiv - Cell Biology 2023Quote: ... which was diluted in culture medium supplemented with 8 µg/ml of protamine sulfate (Sigma-Aldrich, cat.no. P4505-1G). To generate stable cell lines ...
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bioRxiv - Plant Biology 2024Quote: ... Experiments assessing apoplastic pH were carried out as previously described (Barbez et al., 2017) using 8-hydroyypyrene-1,3,6-trisulfonuc acid trisodium salt (HPTS; Sigma-Aldrich). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... and the pellet was resuspended in 2 mL of ChIP buffer (33 mM Tris-HCl pH 8, 100 mM NaCl, 5 mM EDTA, 0.33% SDS, 1.67% Triton X-100, 1X protease inhibitor cocktail [Sigma; P8340]). Chromatin was sheared to approximately 200 bp to 500 bp fragments by sonication using a Bioruptor Plus (Diagenode ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Bioengineering 2024Quote: The fibrin substrate was made using a concentration of 8 mg of fibrinogen from bovine plasma (Sigma Aldrich, F8630) per mL of GM+ to 0.4 U of thrombin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Pancreas islets were isolated from mouse (8 weeks male C57BL/6J) pancreas by collagenase (1 mg/ml, Sigma-Aldrich) injection in the bile duct and handpicked under a binocular microscope (Leica) ...
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The collected clear fluid was transferred into a 1.5 ml reaction tube filled with 8 M urea (Sigma Aldrich). All samples were stored at -20°C until mass spectrometry analysis.
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bioRxiv - Molecular Biology 2023Quote: ... we extracted RNA from 30 individuals fed for 8 days with 5% Sucrose and 0.1% Nipagin in the control group and additionally 2.5nM Paraquat (Sigma 856177) in the treated group ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was mixed with an equal volume of 0.2 M sodium citrate (pH 5.5) and incubated at 37°C for 8 h with or without (as a negative control) trehalase (Sigma). The amount of glucose generated from trehalose was assayed using a Glucose Assay Kit (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The peak fractions were pooled and concentrated to 8-9 mg ml-1 using a 100-kDa concentrator (Millipore) for cryo-EM studies.
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bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
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bioRxiv - Biochemistry 2023Quote: ... Fragmented gDNA or RNase H-digested DNA (4 μg) were immunoprecipitated with 8 µg of S9.6 antibody (Millipore #MABE1095) overnight at 4°C in DRIP binding buffer (10 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then resuspended in transduction medium consisting of ENR medium supplemented with 8 µg/ml polybrene (Sigma-Aldrich), 10 mM of nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers of zebrafish larvae with a SL smaller than 8 mm were directly mounted in Omnipaque350 (Sigma-Aldrich, Histodenz) for imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were infected via centrifugation at 150 xg for 1 hr in 8 µg/mL polybrene (EMD Millipore). After 3 days ...
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bioRxiv - Physiology 2023Quote: ... Male mice (8-9 wks) were treated with a single dose of tamoxifen (40 mg/kg i.p.; Sigma-Aldrich) to induce recombination or corn-oil vehicle as a control at -4 days relative to mini-pump implantation (see below) ...
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bioRxiv - Neuroscience 2023Quote: ... The input sample and washed beads sample were subjected to 8% SDS-PAGE gels and transferred to 0.45 μm PVDF membranes (Millipore), the membrane was blocked by 5% Nonfat dry milk (BIO-RAD ...
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bioRxiv - Developmental Biology 2023Quote: ... filtered through 0.45 μm sterilization filters and added to HC11 cells together with 8 μg/mL polybrene (Sigma-Aldrich). Transduction efficiency was monitored by checking the expression of green fluorescent protein (GFP) ...
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl [pH 8] and 0.1% (w/v) SDS) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and frozen at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... 7 mL of collagen solution was mixed with 800 μL Phenol Red (Sigma-Aldrich, ACS Cas.# 143-74-8) to act as pH indicator ...
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bioRxiv - Cell Biology 2023Quote: ... and the medium was replaced with viral supernatant diluted 1.5-fold with 8 μg/mL polybrene (H9268; Sigma-Aldrich). Two days later ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Cell Biology 2023Quote: ... and the resulting pellets were resuspended in a buffer consisting of 100 µl 8 M Urea (Sigma-Aldrich, #U1250), 100 mM TEAB (triethylammonium bicarbonate ...
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bioRxiv - Biochemistry 2023Quote: ... EA-nap suspension (1 mL) was placed into an ultrafiltration centrifuge tube (interception molecular weight: 8 kDa; Millipore, Ireland), and centrifuged at −4℃ for 20 min at 12,000 r/min ...
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bioRxiv - Microbiology 2023Quote: ... approximately 8 L of seawater was filtered onto 0.22-μm Sterivex-GP pressure filter units (Millipore, Billerica, MA, USA). The filters were immediately soaked in 2 mL of RNAlater Stabilization Solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Viral-containing supernatants were cleared of cellular debris by 0.45 μM filtration and mixed with 8 μg/ml polybrene (Sigma). Target cells were exposed to viral supernatants for two 24 h periods before being washed ...
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bioRxiv - Cell Biology 2024Quote: ... and plated in Induction Medium (Essential 8 Medium,10 μM Chroman1, MedChemExpress, and 2 μg/mL Doxycycline hydrochloride, Sigma) at 400,000 cells per well on double-coated [hESC-qualified Matrigel Matrix and Poly-L-Ornithine (Sigma)] 10 cm tissue-culture treated plates ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from the purified PB or pre-sorting fractions were dissolved in a 8 M urea solution (Sigma-Aldrich) before trypsin digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries and testes were ground using RNase-free 1.5 mL pestles (SLS) in 500 mL lysis buffer B per gradient [50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Genetics 2024Quote: ... The powder obtained was added to 100 μL chondroitinase ABC solution (50 mM Tris HCl pH 8, 50 mM sodium acetate, 0.1 mU/μL chondroitinase ABC (Millipore)) and incubated in a thermomixer set to 37 °C and 1,500 rpm for 6 h ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphorylated-tau 181 (p-Tau) were measured according to manufacturer specifications (Luminex xMAP technology; EMD Millipore, Chicago, IL, USA: HNABTMAG-68K), modified by a 1:10 dilution of CSF ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2019Quote: Synaptoneurosome samples and a protein ladder were separated on 4-20% SDS-PAGE gels (Pierce, Rockford, IL) and transferred to polyvinyldenine fluoride (PVDF) membranes (Millipore, Billerica, MA). The blots were blocked in PBS containing 0.05% Triton-x (Sigma ...