Labshake search
Citations for Millipore Sigma :
4101 - 4150 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... cells that were split a day before were infected with the viral supernatants obtained from the above transfected cells and supplemented with 8 µg of polybrene/ml (Sigma) for at least 6 hours ...
-
bioRxiv - Biochemistry 2019Quote: ... The virus-containing media was passed through a 0.45 μm filter and added to U937 cells in media containing 8 μg/mL polybrene (Sigma Aldrich). The cells were spun at 1000 x g for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: ... Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore) for transduction ...
-
bioRxiv - Immunology 2019Quote: ... Infection of J774A.1 cells with lentiviruses was performed at 50% confluency in a 10 cm-dish with 8 μg polybrene (Sigma) in the absence of FBS ...
-
bioRxiv - Microbiology 2019Quote: ... equal amounts of newly harvested microconidia grown on V8 agar plates for 8 days were cultivated in 0.2x potato dextrose broth (PDB) (Sigma-Aldrich) for 5.5 h and 6.5 h with gentle shaking ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by loading whole cell lysates onto an 8%-10% SDS-PAGE gel for electrophoresis and then transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were incubated with primary antibodies in TBST (TBS ...
-
bioRxiv - Neuroscience 2019Quote: ... in 0.1M PBS and then developed with a 8 min incubation in DAB-Nickel solution: 10mg 3,3′-diaminobenzidine (DAB) tablet (Sigma, DF905), 0.5 ml of a 2% aqueous nickel sulfate solution ...
-
bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
-
bioRxiv - Cancer Biology 2019Quote: ... the viral supernatant was collected and added to cells in six-well plates with medium containing 8 μg/ml polybrene (Sigma). After 2 days’ infection ...
-
bioRxiv - Immunology 2019Quote: ... They were further diluted 1 in 8 in HBSPE running buffer containing 1% (w/v) carboxymethyl-dextran sodium salt (Sigma) and analysed in the Biacore 3000 instrument at a temperature of 25°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Supernatant containing lentiviral particles was used to infect cancer cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich) overnight ...
-
bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
-
bioRxiv - Immunology 2021Quote: ... the semi-dry protein pellet was dissolved in 25µL of 8 M urea in 20mM EPPS (pH 8.5) (Sigma; Cat#E9502) and was then diluted with EPPS buffer to reduce urea concentration to 4 M ...
-
bioRxiv - Neuroscience 2020Quote: ... we prepared solutions of 8 mg/mL antibody in borate buffer (100 mM borate, 2 mM EDTA, pH 8.0; all Sigma-Aldrich). The antibody concentrations were determined from the absorbance at 280 nm (NanoDrop 2000c spectrophotometer ...
-
bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
-
bioRxiv - Molecular Biology 2021Quote: Type 1 diabetes was induced in male Sprague Dawley rats at 8 weeks of age via a single 55mg/kg tail vein injection of streptozotocin (STZ; Sigma), and monitored for 8 weeks ...
-
bioRxiv - Biochemistry 2021Quote: A431 cell pellets were dispersed in lysis buffer (Tris 50 mM pH = 8, 150 mM NaCl, 1% IGEPAL + protease inhibitor cocktail P8340 from Sigma) and incubated on ice with occasional vortexing for 15 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Cells were transduced with the appropriate lentivirus stock for 48h in the presence of 8 μg/mL of polybrene (Millipore) prior to selection with puromycin.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were seeded into 6-well plates to reach 50-60% confluency on the day of infection and were transduced 2-3 consecutive days with the viral supernatant in the presence of 8 μg/mL polybrene (Sigma). The viral supernatant was replaced with fresh culture medium ...
-
bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
-
bioRxiv - Cell Biology 2021Quote: ... the HEK293T cell lysates transiently expressing mScarlet-I-tagged spartin truncation mutants were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
-
bioRxiv - Cell Biology 2021Quote: ... 8-chamber coverglass slides (155411, ThermoScientific) were coated with 1 mg/ml of lectin from glycine max soybean (L1395, Sigma) and left for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... The immunoprecipitates were applied to 8 % SDS-PAGE and the gel was stained by ProteoSilver™ stain kit (Sigma-Aldrich) according to manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
-
bioRxiv - Microbiology 2020Quote: Cell lysate was prepared using lysis buffer (50mM Tris, 150mM NaCl, 1% Triton, pH=8) supplemented with Phosphatase Inhibitor Cocktail (Sigma) at a concentration of 4×106 cells in 100 μL of buffer ...
-
bioRxiv - Systems Biology 2020Quote: ... were used for binding assays with a constant amount of fluorescein isothiocyanate (FITC)-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 10 nM) ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
-
bioRxiv - Microbiology 2021Quote: GFP-LC3 HeLa cells or HeLa-Difluo hLC3 cells were seeded onto Nunc Lab-Tek Chamber Slide system 8 wells (Sigma). The day of the experiment ...
-
bioRxiv - Neuroscience 2020Quote: ... Synaptic varicosities were labeled with primary antibody for vesicular glutamate transporter 1 (vGluT1; 1:2000, 8 days incubation, Guinea Pig, MilliPore Biosciece Research Reagent ...
-
bioRxiv - Microbiology 2021Quote: ... were transduced with the sgRNA library lentivirus at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described [36] ...
-
bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
-
bioRxiv - Cancer Biology 2021Quote: ... were seeded in 6-well plates before addition of fresh medium containing viral supernatant (~5×105 transducing units) and Polybrene (8 μg/mL; Sigma) and ‘spin-oculated’ for 90 min at 1,500 rpm ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cancer cells were grown in 6-well plates for 24 hours and transduced with 500 μL of lentivirus aliquot diluted in cell-specific medium in the presence of 8 μg/mL of Sequebrene (Sigma) and incubated at 37°C and 5% CO2 for 72 hrs ...
-
bioRxiv - Cell Biology 2021Quote: ... Click-it chemistry-based azide coupling was performed by incubation for 1 hour with 60 µM Atto594 Azide (Attotec, Germany) in 50 mM Tris buffer (pH 8) with 4 mM CuSO4 (Sigma), and 10 mM freshly prepared ascorbic acid (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... The 8 well dishes were plasma activated for 30 seconds and coated with either 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) in PBS or 20 μg/ml laminin (Merck Millipore ...
-
bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μl of each fraction was spotted on to a 10 x 8 cm piece of TLC PEI Cellulose F paper (Millipore) 1 cm above the bottom of the paper and developed for 10 mins using 400 mM NaH2PO4 pH 3.6 as the solvent ...
-
bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The cell pellet was resuspended in 1 mL TM2+PI (10mM Tris pH 8, 2mM MgCl2 + Protease Inhibitor, Sigma 11836170001) and chilled in ice water for 1 min ...
-
bioRxiv - Cell Biology 2022Quote: ... 70,000-100,000 LECs/well were plated on Matrigel-coated 48-well-plates or Millicell EZ 8-well glass slides (Millipore, LabTek). LECs were allowed to adhere and form tubes for 4-6 hours before medium was aspirated and small intestinal crypts or single cells from organoids were plated atop in 20µl Matrigel domes mixed with organoid media.
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
-
bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
-
bioRxiv - Cell Biology 2022Quote: ... Viruses were harvested 48 hrs and 72 hrs after transfection and transduced in HeLa or HEK293T cells with 8 μg/mL polybrene (Sigma). Cells were then directly used in experiments or optimized for expression by FACS.
-
bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
-
bioRxiv - Neuroscience 2022Quote: Tissues from Tmem150c-/- mutants and wild type mice were lysed with 8 M urea buffer and protein concentrations were determined using the Bradford reagent (Sigma). Proteins were separated by SDS-PAGE ...
-
bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...